Mechanism of antagonistic effects of Andrographis paniculata methanolic extract against Staphylococcus aureus

Hussain, Roslinah Mohamad; Razak, Zayan Nabilah Rasyidah Abd.; Saad, Wan Mazlina Md; Mustakim, Maimunah

doi:10.1016/j.apjtm.2017.07.009

Cited by 16 publications

(13 citation statements)

References 75 publications

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“…This plant has been used in traditional medicine in Asia to treat inflammatory diseases such as respiratory disorders [ 10 ]. Methanolic extract (ME) from the leaves of Andrographis paniculata has been shown to have antioxidant and anti-inflammatory activities [ 11 , 12 , 13 , 14 ]. Andrographolide (ANDRO) is the main bioactive compound of Andrographis paniculata [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Andrographis paniculata and Its Bioactive Diterpenoids Against Inflammation and Oxidative Stress in Keratinocytes

et al. 2020

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Andrographis paniculata was widely used in traditional herbal medicine to treat various diseases. This study explored the potential anti-aging activity of Andrographis paniculata in cutaneous cells. Human, adult, low calcium, high temperature (HaCaT) cells were treated with methanolic extract (ME), andrographolide (ANDRO), neoandrographolide (NEO), 14-deoxyandrographolide (14DAP) and 14-deoxy-11,12-didehydroandrographolide (14DAP11-12). Oxidative stress and inflammation were induced by hydrogen peroxide and lipopolysaccharide/TNF-α, respectively. Reactive oxygen species (ROS) production was measured by fluorescence using a 2′,7′-dichlorofluorescein diacetate (DCFH-DA) probe and cytokines were quantified by ELISA for interleukin-8 (IL-8) or reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for tumor necrosis factor-α (TNF-α). Hyaluronic acid (HA) secretion was determined by an ELISA. Our results show a decrease in ROS production and TNF-α expression by ME (5 µg/mL) in HaCaT under pro-oxidant and pro-inflammatory conditions, respectively. ME protected HaCaT against oxidative stress and inflammation. Our findings confirm that ME can be used for the development of bioactive compounds against epidermal damage.

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Section: Introductionmentioning

confidence: 99%

Andrographis paniculata and Its Bioactive Diterpenoids Against Inflammation and Oxidative Stress in Keratinocytes

et al. 2020

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“…Methanol extract (ME) from the leaves of Andrographis paniculata has been shown to be anti-inflammatory [32], antioxidant [33,34] and antitumor [35]. The same extract showed a hepatoprotective [36] and antiviral effect [37].…”

Section: Introductionmentioning

confidence: 99%

Andrographis paniculata and Its Bioactive Diterpenoids Protect Dermal Fibroblasts against Inflammation and Oxidative Stress

et al. 2020

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Andrographis paniculata (Burm.f.) has long been used in ayurvedic medicine through its anti-inflammatory properties. However, its protective effect of skin aging has not been studied in vitro. This study aimed to investigate the anti-aging effects of methanolic extract (ME), andrographolide (ANDRO), neoandrographolide (NEO), 14-deoxyandrographolide (14DAP) and 14-deoxy-11,12-didehydroandrographolide (14DAP11-12) on human dermal fibroblasts (HDFa) under pro-oxidant or pro-inflammatory condition. The in vitro anti-aging capacity of ME, ANDRO, NEO, 14DAP, and 14DAP11-12 (1, 2.5 and 5 µg/mL) was performed in HDFa. Oxidative stress and inflammation were induced by hydrogen peroxide and lipopolysaccharide/TNF-α, respectively. Reactive oxygen species (ROS) production was measured by the fluorescence of DCF-DA probe and cytokines were quantified by ELISA (IL6 and IL8) or RTqPCR (TNF-α). Procollagen type I production was determined by an ELISA. Our results showed a decrease in ROS production with ME and 14DAP at 5 µg/mL and 1 µg/mL, respectively. Furthermore, IL-6 production and TNF-α expression decreased under ANDRO and ME at 5 µg/mL. Our data indicated that ME and 14DAP protect from oxidative stress. Additionally, ME and ANDRO decreased an inflammation marker, IL-6. This suggests their potential natural treatment against skin damage. Hence, their applications could be of interest in cosmetics for preventing skin ageing.

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“…Suppression of SOD activity results in decreased conversion of O 2 -to H 2 O 2 and consequently resulting in increased O 2 -levels and leads to oxidative stress mediated toxicity in S. aureus cells. Early reports demonstrated decreases in SOD activity in E. coli and S. aureus upon treatment with methanolic extract of A. paniculata, 2phenylethynylbutyltellurium (PEBT) and catechin [42][43][44] .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and Antioxidant Activities of Extracts and Compounds From Trifolium Baccarinii With Their Mechanisms of Antibacterial Action

Feugap¹,

Kengne²,

Tamokou³

et al. 2020

Preprint

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The treatment of infectious diseases with antimicrobial agents continues to present problems in modern-day medicine with many studies showing significant increase in the incidence of bacterial resistance to several antibiotics. The screening of plant extracts and natural compounds for antimicrobial activity has demonstrated that higher plants represent a potential source of new anti-infective agents. The aim of this study was to evaluate the antimicrobial and antioxidant activities of extracts and compounds from the whole plant Trifolium baccarinii Chiov. with their mechanisms of antibacterial action. Biochanin A (1), formononetin (2), luteolin (3), luteolin-4'-O-β-D-glucopyranoside (4), 4,7,2'-trihydroxy-4'-methoxyisoflavanol (5), sissotrin (6), 1-méthyl-β-D-glucopyranoside (7), ononin (8), D-mannitol (9) and 3-O-β-D-glucuronopyranosylsoyasapogenol B (10) were isolated from T. baccarinii. The MeOH, EtOAc and n-BuOH extracts as well as compounds 1–6 from T. baccarinii displayed the most antimicrobial and antioxidant activities. The MeOH extract and compound 5 exhibited antibacterial activity through bacteriolytic effect and reduction of the antioxidant defenses in the bacterial cells. Interestingly, none of the tested samples showed cytotoxic activity against normal cells; highlighting their good selectivity toward pathogenic bacteria and yeasts. Hence, they are promising lead candidates with antibacterial potential against methicillin resistant S. aureus (MRSA).

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Mechanism of antagonistic effects of Andrographis paniculata methanolic extract against Staphylococcus aureus

Cited by 16 publications

References 75 publications

Andrographis paniculata and Its Bioactive Diterpenoids Against Inflammation and Oxidative Stress in Keratinocytes

Andrographis paniculata and Its Bioactive Diterpenoids Against Inflammation and Oxidative Stress in Keratinocytes

Andrographis paniculata and Its Bioactive Diterpenoids Protect Dermal Fibroblasts against Inflammation and Oxidative Stress

Antimicrobial and Antioxidant Activities of Extracts and Compounds From Trifolium Baccarinii With Their Mechanisms of Antibacterial Action

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