Andrographis paniculata and Its Bioactive Diterpenoids Protect Dermal Fibroblasts against Inflammation and Oxidative Stress

Abstract

Andrographis paniculata (Burm.f.) has long been used in ayurvedic medicine through its anti-inflammatory properties. However, its protective effect of skin aging has not been studied in vitro. This study aimed to investigate the anti-aging effects of methanolic extract (ME), andrographolide (ANDRO), neoandrographolide (NEO), 14-deoxyandrographolide (14DAP) and 14-deoxy-11,12-didehydroandrographolide (14DAP11-12) on human dermal fibroblasts (HDFa) under pro-oxidant or pro-inflammatory condition. The in vitro anti-aging capacity of ME, ANDRO, NEO, 14DAP, and 14DAP11-12 (1, 2.5 and 5 µg/mL) was performed in HDFa. Oxidative stress and inflammation were induced by hydrogen peroxide and lipopolysaccharide/TNF-α, respectively. Reactive oxygen species (ROS) production was measured by the fluorescence of DCF-DA probe and cytokines were quantified by ELISA (IL6 and IL8) or RTqPCR (TNF-α). Procollagen type I production was determined by an ELISA. Our results showed a decrease in ROS production with ME and 14DAP at 5 µg/mL and 1 µg/mL, respectively. Furthermore, IL-6 production and TNF-α expression decreased under ANDRO and ME at 5 µg/mL. Our data indicated that ME and 14DAP protect from oxidative stress. Additionally, ME and ANDRO decreased an inflammation marker, IL-6. This suggests their potential natural treatment against skin damage. Hence, their applications could be of interest in cosmetics for preventing skin ageing.

Andrographis paniculata and Its Bioactive Diterpenoids Against Inflammation and Oxidative Stress in Keratinocytes

“…In methanolic extract, a higher concentration of ANDRO and 14DAP11-12 was found [ 31 ]. In a primary culture of human dermal fibroblasts (HDFa), ME (5 µg/mL) and 14DAP (1 µg/mL) decreased ROS production under oxidative stress conditions [ 14 ]. Andrographolide sodium bisulfate is a soluble product derived from ANDRO.…”

“…This plant has been used in traditional medicine in Asia to treat inflammatory diseases such as respiratory disorders [ 10 ]. Methanolic extract (ME) from the leaves of Andrographis paniculata has been shown to have antioxidant and anti-inflammatory activities [ 11 , 12 , 13 , 14 ]. Andrographolide (ANDRO) is the main bioactive compound of Andrographis paniculata [ 15 ].…”

“…Andrographolide (ANDRO) is the main bioactive compound of Andrographis paniculata [ 15 ]. In the context of skin aging, ANDRO is interesting for its antioxidant and anti-inflammatory activities [ 14 , 16 , 17 , 18 ]. ANDRO is a diterpenoid, like neoandrographolide (NEO), 14-deoxyandrographolide (14DAP), and 14-deoxy-11,12-didehydroandrographolide (14DAP11-12).…”

“…ME and 14DAP have been shown to decrease ROS production under oxidative stress conditions, as well as IL-6 secretion and TNF-α expression under inflammatory conditions, in dermal fibroblasts [ 14 ]. However, there is poor information on epidermal cells and keratinocytes.…”

The Potential of Sulfated Polysaccharides Isolated from the Brown Seaweed Ecklonia maxima in Cosmetics: Antioxidant, Anti-melanogenesis, and Photoprotective Activities

“…Thus, it is continually damaged by environmental factors such as chemicals, fine dust particles, and ultraviolet (UV) irradiation [1]. These environmental factors cause various effects on the skin including oxidative stress, inflammatory responses, melanin accumulation, and collagen degradation, leading to skin aging [2][3][4][5]. The appearance of the skin directly contributes to personal beauty [6].…”