Mechanism of action of DNA-hydrolyzing antibodies to DNA from blood of patients with systemic lupus erythematosus

Abstract

Four fractions of IgG antibodies to native DNA (nDNA) were obtained from blood of patients with systemic lupus erythematosus (SLE). These antibodies displayed a thermostable DNA-hydrolyzing activity and were different in affinity for DNA-cellulose and sorption on DEAE-cellulose. DNA-hydrolyzing antibodies to nDNA are metal-dependent endonucleases, cause mainly single-strand breaks in DNA, and are active over a wide range of pH. By atomic-force microscopy, three-dimensional images of DNA complexes with DNA-hydrolyzing antibodies to nDNA were obtained with nanometer resolution, and a nonprocessive action mechanism was shown for the DNase activity of antibodies to nDNA.

Invasive fungal infection in patients with systemic lupus erythematosus: Experience from a single institute of Northern China

“…In the SLE patients, it is reported that the clinical use of glucocorticoid or other immunosuppressants, multiple antibiotics and steroidinduced diabetes always contributed to the dysfunction of immune response. And the dysfunction of immune cells including T cell, B cell and dendritic cell was demonstrated to promote SLE patients more susceptible for the occurrence of IFI (Nambiar et al, 2002;Nevzorova et al, 2006;Ramanujam et al, 2004;Reefman et al, 2007;Seta et al, 2002;Takeuchi, 2002;Traustadottir et al, 2002). Therefore, it is believed that the imbalancement of immune response of host be capable of increasing the risk for the incidence of IFI (Petri and Genovese, 1992).…”