Mechanism for the inhibitory effect of dextran on α2-plasmin inhibitor activity

Using casein plates as a sensitive assay for proteolytic activity, it was observed that sodium-dodecyl sulfate (SDS) and other anionic detergents induce caseinolysis when mixed with sera and plasma. Caseinolysis was dependent on the presence of plasminogen in the fluids and could be blocked by inhibitors of serine proteases and antibody to plasminogen. Similarly, organic solvents such as isopropanol induced caseinolysis after mixing with plasma, but not normal serum. Isopropanol dissociated complexes of alpha 1-antitrypsin or alpha 2-macroglobulin with trypsin preformed in vitro. As both SDS and organic solvents are widely used in biochemical investigations of biological fluids, attention should be paid to the possible induction of proteolysis.

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Effets des solutés de remplissage vasculaire sur l’hémostase

Blanlœil¹,

Trossaërt²,

Rigal³

et al. 2002

Annales Françaises d'Anesthésie et de Réanimation

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Mechanism for the inhibitory effect of dextran on α2-plasmin inhibitor activity

Cited by 6 publications

References 17 publications

Panel II: Dosage and scheduling regimens for erythrocyte‐sedimenting macromolecules

Panel II: Dosage and scheduling regimens for erythrocyte‐sedimenting macromolecules

Induction of proteolytic activity in serum by treatment with anionic detergents and organic solvents

Effets des solutés de remplissage vasculaire sur l’hémostase

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