Mechanical Trap Surface‐Enhanced Raman Spectroscopy for Three‐Dimensional Surface Molecular Imaging of Single Live Cells

Abstract

Reported is a new shell-based spectroscopic platform, named mechanical trap surface-enhanced Raman spectroscopy (MTSERS), for simultaneous capture, profiling, and 3D microscopic mapping of the intrinsic molecular signatures on the membrane of single live cells. By leveraging the functionalization of the inner surfaces of the MTs with plasmonic gold nanostars, and conformal contact of the cell membrane, MTSERS permits excellent signal enhancement, reliably detects molecular signatures, and allows non-perturbative, multiplex 3D surface imaging of analytes, such as lipids and proteins on the surface of single cells. The demonstrated ability underscores the potential of MTSERS to perform 3D spectroscopic microimaging and to furnish biologically interpretable, quantitative, and dynamic molecular maps in live cell populations.

Engineering State‐of‐the‐Art Plasmonic Nanomaterials for SERS‐Based Clinical Liquid Biopsy Applications

“…This gold nanoparticle-functionalized Si nanorod array produced a long decay length of >130 nm (Figure 8c,d). [69] The functionalization of inner surfaces of glasslike mechanical traps with gold nanostars and the conformal contact of cell membranes enable sensitive, nonperturbative, and multiplex 3D surface molecular analysis of single cells (not achievable by use of a 2D SERS substrate). [69] The functionalization of inner surfaces of glasslike mechanical traps with gold nanostars and the conformal contact of cell membranes enable sensitive, nonperturbative, and multiplex 3D surface molecular analysis of single cells (not achievable by use of a 2D SERS substrate).…”

“…Reproduced with permission. [69] Copyright 2017, Wiley-VCH. b) Gold nanoparticle-decorated ZnO nanorods grown on cellulose paper for SERS analyses of amniotic fluids to detect prenatal diseases.…”

Optical Imaging Approaches to Monitor Static and Dynamic Cell‐on‐Chip Platforms: A Tutorial Review

“…Raman scattering is usually collected in a 180‐degree backscattered configuration and thus, a wide range of specimens could be mounted on the imaging platform. Jin et al used Raman microscopy for 3D surface molecular imaging of breast cancer cells captured in a microfluidic cell trap device . The detection of the low vibrational shifts in light signals is a technical challenge for arranging these imaging approaches.…”

“…j) Raman microscopy image of a single breast cancer cell captured in a mechanical trap on a chip. Reproduced with permission . Copyright 2017, WILEY‐VCH.…”

Elektrochemilumineszenz‐Bioassays können Fluoreszenzassays mithilfe eines wasserlöslichen Luminolderivats übertreffen

“…[3] Luminol-ECL wurde zum ersten Mal 1928 beschrieben. [5] Hier kann die Kombination der praktisch hintergrundfreien ECL zusammen mit einer Signalverstärkung durch Nanovesikel wie Liposome [6] unerreichte Mçglichkeiten bieten. Des Weiteren wird der Einsatz von transparenten Elektrodenmaterialien, z.…”