Optical Imaging Approaches to Monitor Static and Dynamic Cell‐on‐Chip Platforms: A Tutorial Review

Arandian, Alireza; Bagheri, Zeinab; Ehtesabi, Hamide; Nobar, Shima Najafi; Aminoroaya, Neda; Samimi, Ashkan; Latifi, Hamid

doi:10.1002/smll.201900737

Cited by 35 publications

(24 citation statements)

References 147 publications

(257 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The time of the cell loading and fresh culture medium change is 60 s. The culture medium was exchanged once a day to ensure sufficient nutrient supply to the cells. Spheroid growth tracking overtime was also examined by everyday imaging 50 . Bright-field and epi-fluorescence imaging were conducted on a Zeiss Axiovert 40 CFL inverted routine microscope.…”

Section: Pdms Casting and Device Assemblymentioning

confidence: 99%

A combined 3D printing/CNC micro-milling method to fabricate a large-scale microfluidic device with the small size 3D architectures: an application for tumor spheroid production

Behroodi

Latifi

Bagheri

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

The fabrication of a large-scale microfluidic mold with 3D microstructures for manufacturing of the conical microwell chip using a combined projection micro-stereolithography (PµSL) 3D printing/CNC micro-milling method for tumor spheroid formation is presented. The PµSL technique is known as the most promising method of manufacturing microfluidic chips due to the possibility of creating complex three-dimensional microstructures with high resolution in the range of several micrometers. The purpose of applying the proposed method is to investigate the influence of microwell depths on the formation of tumor spheroids. In the conventional methods, the construction of three-dimensional microstructures and multi-height chips is difficult, time-consuming, and is performed using a multi-step lithography process. Microwell depth is an essential parameter for microwell design since it directly affects the shear stress of the fluid flow and the diffusion of nutrients, respiratory gases, and growth factors. In this study, a chip was made with microwells of different depth varying from 100 to 500 µm. The mold of the microwell section is printed by the lab-made PµSL printer with 6 and 1 µm lateral and vertical resolutions. Other parts of the mold, such as the main chamber and micro-channels, were manufactured using the CNC micro-milling method. Finally, different parts of the master mold were assembled and used for PDMS casting. The proposed technique drastically simplifies the fabrication and rapid prototyping of large-scale microfluidic devices with high-resolution microstructures by combining 3D printing with the CNC micro-milling method.

show abstract

Section: Pdms Casting and Device Assemblymentioning

confidence: 99%

A combined 3D printing/CNC micro-milling method to fabricate a large-scale microfluidic device with the small size 3D architectures: an application for tumor spheroid production

Behroodi

Latifi

Bagheri

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Shadows of small objects are also used for holographic microscopy and on-chip shadow imaging [ 74 ]. In shadow imaging, any optically semi-transparent micro-object such as cells is directly placed on top of an optoelectronic image sensor and illuminated by an incoherently or partially coherent light source.…”

Section: Optical Techniquesmentioning

confidence: 99%

Applications of CMOS Devices for the Diagnosis and Control of Infectious Diseases

Forouhi

Ghafar-Zadeh

2020

Micromachines

View full text Add to dashboard Cite

Emerging infectious diseases such as coronavirus disease of 2019 (COVID-19), Ebola, influenza A, severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) in recent years have threatened the health and security of the global community as one of the greatest factors of mortality in the world. Accurate and immediate diagnosis of infectious agents and symptoms is a key to control the outbreak of these diseases. Rapid advances in complementary metal-oxide-semiconductor (CMOS) technology offers great advantages like high accuracy, high throughput and rapid measurements in biomedical research and disease diagnosis. These features as well as low cost, low power and scalability of CMOS technology can pave the way for the development of powerful devices such as point-of-care (PoC) systems, lab-on-chip (LoC) platforms and symptom screening devices for accurate and timely diagnosis of infectious diseases. This paper is an overview of different CMOS-based devices such as optical, electrochemical, magnetic and mechanical sensors developed by researchers to mitigate the problems associated with these diseases.

show abstract

“…Sensors have been particularly developed focusing on miniaturization by the use of novel materials for fabricating sensors. The interesting characteristics of nanomaterials are illustrated in their combined advantages of a tiny size with an extreme increase in the surface area, rendering them a tremendous potential for versatile applications [ [7] , [8] , [9] , [10] ]. The nano-sized nature of nanomaterials and their exclusive optical, electronic, magnetic, and mechanical attributes can enhance patient care through the use of sensors with minimal invasivity and extreme sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Application of carbon nanomaterials in human virus detection

Ehtesabi

2020

Journal of Science: Advanced Materials and Devices

Self Cite

View full text Add to dashboard Cite

Human-pathogenic viruses are still a chief reason for illness and death on the globe, as epitomized by the COVID-19 pandemic instigated by a coronavirus in 2020. Multiple novel sensors have been invented because diseases must be detected and diagnosed as early as possible, and recognition methods have to be carried out with minimal invasivity. Sensors have been particularly developed focusing on miniaturization by the use of nanomaterials for fabricating nanosensors. The nano-sized nature of nanomaterials and their exclusive optical, electronical, magnetical, and mechanical attributes can enhance patient care through the use of sensors with minimal invasivity and extreme sensitivity. Amongst the nanomaterials utilized for fabricating nano-sensors, carbon-based nanomaterials are promising as these sensors respond better to signals in various sensing settings. This review provides an overview of the recent developments in carbon nanomaterial-based biosensors for viral recognition based on the biomarkers that arise from the infection, the nucleic acids from the viruses, and the entire virus. The role of carbon nanomaterials is highlighted by the improvement of sensor and recognition functionality. The Dengue virus, Ebola virus, Hepatits virus, human immunodeficiency virus (HIV), influenza virus, Zika virus and Adenovirus are the virus types reviewed to illustrate the implementation of the techniques. Finally, the drawbacks and advantages of carbon nanomaterial-based biosensors for viral recognition are identified and discussed.

show abstract

Optical Imaging Approaches to Monitor Static and Dynamic Cell‐on‐Chip Platforms: A Tutorial Review

Cited by 35 publications

References 147 publications

A combined 3D printing/CNC micro-milling method to fabricate a large-scale microfluidic device with the small size 3D architectures: an application for tumor spheroid production

A combined 3D printing/CNC micro-milling method to fabricate a large-scale microfluidic device with the small size 3D architectures: an application for tumor spheroid production

Applications of CMOS Devices for the Diagnosis and Control of Infectious Diseases

Application of carbon nanomaterials in human virus detection

Contact Info

Product

Resources

About