Mechanical properties of adsorption layers in solutions of human blood serum proteins: A comparative assessment

Yakhno, Tatyana; Казаков, В. В.; Sanin, A.; Shaposhnikova, O. B.; Чернов, А. В.

doi:10.1134/s1063784207040184

Cited by 6 publications

(9 citation statements)

References 3 publications

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“…So, salt crystallization becomes more rapid (SI-1 decreases). The lowring in surface tension of HSA solutions after adding immunoglobulines, as well as rise in surface tension after adding Fn, were shown by direct measurements (Yakhno et al, 2007a). In addition, we tested BSA solutions (70 g l -1 ) in either NaCl or KCl water solutions (9 g l -1 ).…”

Section: Resultsmentioning

confidence: 99%

“…These aptitude tests used a drying drop as a "black box" with very complex processes into it during drying. We come to know the particulars of these processes using model protein and salt water solutions, which mimic human serum (Yakhno et al, 2007a;Yakhno et al, 2007b;Yakhno, 2008). As long as some deseases are accompanied by high level of immunoglobulines, and different levels of fibronectin (Fn) in blood serum (Heil, W. et al, 1999), we would like to see what changes in shape of the AMI curves take place when some of these serum components are added to the Human Serum Albumin (HSA) solution.…”

Section: Introductionmentioning

confidence: 99%

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Uncoated Quartz Resonator as a Universal Biosensor

Yakhno¹,

Sanin²,

Казаков³

et al. 2010

Intelligent and Biosensors

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Uncoated Quartz Resonator as a Universal Biosensor

Yakhno¹,

Sanin²,

Казаков³

et al. 2010

Intelligent and Biosensors

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“…The protein ring in dried drops moves to the edge when the salt/protein ratio in the initial solution increases (Figure 2). Earlier we found that IgG, IgM and Fn exert a considerable effect on both, the morphological and dynamic parameters of fluid structuring in drying drops through a change in the surface tension and viscoelastic properties of the adsorption layer at the fluid-air interface [25,26]. The goal of the current work is to elucidate how these macromolecular serum components influence protein structure formation during drop drying.…”

Section: Resultsmentioning

confidence: 93%

Protein phase instability developed in plasma of sick patients: clinical observations and model experiments

Yakhno¹

2010

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This article discusses the causes of formation of micron-size protein structures in liquid plasma or serum of the patients with different diseases, which are accompanied by inflammatory reac-tions. Self-organizing processes in sessile dry-ing drops of natural and model biological liq-uids are used for study of possible mechanisms of development the protein phase instability in serum. There was shown that violation of opti-mal ratio between albumin and osmotic active components could lead to loss of albumin ag-gregative stability and albumin coagulation structures formation. Possible role of these structures in pathogenesis of inflammation is discussed

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“…Additionally, a positive correlation of surface tension was found with the total content of seromucoids, which comprise at least 15 various carbohydrate and protein complexes, including prealbumin, alpha-1-acidic glycoprotein, haptoglobin, etc. The high molecular weight proteins of biological fluids have higher surface activity when compared to albumin, which provides for their advantage in the formation of adsorption layers at the air boundary [54]. In this connection, the existence of a negative correlation of the average molecular weight protein toxins and immunoglobulins with surface tension is logical.…”

Section: Discussionmentioning

confidence: 99%

Morphology of Dried Drop Patterns of Saliva from a Healthy Individual Depending on the Dynamics of Its Surface Tension

2019

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Background: The study of processes in the drying drops of biological fluids (dried drop patterns) and the method of dynamic surface tensiometry can be attributed to integral methods of assessing the state of the organism. Research objective: to establish the relationship between the type of crystallization patterns and the surface tension of human saliva in normal conditions. Methods: 100 volunteers (40 males, 60 females) that were aged 30–59 participated in the study. In all saliva samples, the parameters of dynamic tensiometry, types of crystallization patterns and 11 biochemical parameters were determined. Results: No statistically significant differences in the saliva crystallization patterns were observed, depending on the age and gender characteristics of the volunteers. A negative correlation of the area of the crystallization zone and the surface tension of saliva is shown. When considering the crystallization patterns, their considerable variability was noted; on this basis, the entire studied sample was divided into four clusters by surface tension. Conclusion: In general, the crystallization patterns that are inside the selected groups remain quite heterogeneous. This increases the likelihood of making an incorrect diagnosis when using visual methods to evaluate the crystallization patterns, which significantly limits the use of such diagnostic methods in clinical practice.

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Mechanical properties of adsorption layers in solutions of human blood serum proteins: A comparative assessment

Cited by 6 publications

References 3 publications

Uncoated Quartz Resonator as a Universal Biosensor

Uncoated Quartz Resonator as a Universal Biosensor

Protein phase instability developed in plasma of sick patients: clinical observations and model experiments

Morphology of Dried Drop Patterns of Saliva from a Healthy Individual Depending on the Dynamics of Its Surface Tension

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