Mechanical Fragility Calibration of Red Blood Cells

Gu, Lei; Smith, William A.; Chatzimavroudis, George P.

doi:10.1097/01.mat.0000161940.30190.6d

Cited by 28 publications

(28 citation statements)

References 14 publications

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“…The deleterious effects of blood pumps, cardiac valves, and even needles on erythrocytes are well characterized. The cell damage is known to be a function of several parameters, among them shear stress, exposure time, blood contact material, and blood composition (14 ). For a cannula it has been shown that most of the hemolysis is produced on the external edge of the tip of the cannula, as the blood makes a 180-degree turn (15 ).…”

Section: Resultsmentioning

confidence: 99%

Determination of Hemolysis Thresholds by the Use of Data Loggers in Pneumatic Tube Systems

et al. 2011

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BACKGROUND Pneumatic tube systems (PTSs) for the transport of blood samples are regaining popularity in medical centers after earlier reports that their use could introduce preanalytical distortions such as hemolysis and changes in blood gases. METHODS We drew duplicate blood samples from 30 volunteers. One sample was hand transported, and the other sample was transported through a PTS together with a mini–data logger that provided continuous measurements of temperature, humidity, pressure, and acceleration. After transport the samples were analyzed at the same time. We looked for possible relationships of the transport method and the parameters measured by the data loggers with differences in hematological parameters, standard clinical chemistry analyses, blood coagulation, erythrocyte sedimentation rate, and blood gas analysis. RESULTS There were no significant differences in temperature, humidity, and pressure between the methods of transport, but we observed significant differences in 3-axis accelerations. The combined effect of these forces could be described by the right-tailed area under the vector sum acceleration distribution. Our data show that this area correlated with PTS speed and that PTS speed and the area under the curve exhibited a direct relation to the degree of hemolysis. CONCLUSIONS Assessment of 3-axis acceleration by use of data loggers can be used to identify preanalytical deviations that result from the transportation of blood samples in PTSs. Our approach could be used for the evaluation and regular control of PTSs without the need for repeated blood drawing and laboratory analyses.

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Section: Resultsmentioning

confidence: 99%

Determination of Hemolysis Thresholds by the Use of Data Loggers in Pneumatic Tube Systems

et al. 2011

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“…The flow velocity used in this study was 0.02 m s −1 , which was limited by the shutter time of the camera. When the channel width was smaller than 10 μm, the highest shear stress increased significantly to 300 Pa, sufficiently high for causing RBC lysis 21,22 . When the channel width was larger than 14 μm, the shear stress was not sufficient to bend the RBCs.…”

Section: Methodsmentioning

confidence: 99%

“…However, RBC deflection increased linearly only when the flow velocity was lower than 0.06 m s −1 , after which nonlinearity occurred. This is because the stress induced by a flow velocity higher than 0.06 m s −1 exceeds RBC's yield stress (250 Pa to 300 Pa) 21,22,28 . Thus, a flow velocity o0.03 m s −1 was used in the experiments and was not considered to be sufficiently high enough to induce nonlinearity in the RBC effective stiffness.…”

Section: Methodsmentioning

confidence: 99%

Stiffness increase of red blood cells during storage

Zheng

Wang

et al. 2018

Microsyst Nanoeng

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In transfusion medicine, the deformability of stored red blood cells (RBCs) changes during storage in blood banks. Compromised RBC deformability can reduce the transfusion efficiency or intensify transfusion complications, such as sepsis. This paper reports the microfluidic mechanical measurement of stored RBCs under the physiological deformation mode (that is, folding). Instead of using phenomenological metrics of deformation or elongation indices (DI or EI), the effective stiffness of RBCs, a flow velocityindependent parameter, is defined and used for the first time to evaluate the mechanical degradation of RBCs during storage. Fresh RBCs and RBCs stored up to 6 weeks (42 days) in the blood bank were measured, revealing that the effective stiffness of RBCs increases over the storage process. RBCs stored for 1 week started to show significantly higher stiffness than fresh RBCs, and stored RBC stiffness degraded faster during the last 3 weeks than during the first 3 weeks. Furthermore, the results indicate that the time points of the effective stiffness increase coincide well with the degradation patterns of S-nitrosothiols (SNO) and adenosine triphosphate (ATP) in RBC storage lesions.

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“…K 50 is the slope at this point. For all 6 units tested, quadraticallyinterpolated S 50 and corresponding K 50 values are listed in Table 1; the data also suggest some potential independence between these two values. While such characteristics permit straightforward comparisons, more complex profiles may reveal more meaningful patterns in stress susceptibilities.…”

Section: Figurementioning

confidence: 99%

“…Regarding mechanical fragility, one limitation on its adoptability for standardized clinical applications appears to have been a lack of a testing means that is both highly sensitive and clinically practical (50). However, we believe this erythrocyte property to be a particularly likely measure of membrane state to correlate with transfusion efficacy: Mechanical fragility represents a sample's propensity for hemolysis under sustained and varied physical stressesoffering greater potential, with proper configuration, to resemble the physiological stresses cells experience in vivo.…”

Section: Researching Candidate Metric(s)mentioning

confidence: 99%

Investigating Direct Non-Age Metrics of Stored Blood Quality Loss

Alfano

Tarasev²

2011

IJMT

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DOI: 10.5580/2789Abstract Long storage times for blood products are often unavoidable. Product age is essentially the only indicator used today for Red Blood Cell (RBC) quality loss during storage. Much controversy persists over the impact of RBC age on transfusion outcomes, as studies on this remain inconclusive. Such inconsistency may arise from unit-to-unit variability, which likely introduces some age-independence to RBC state. Thus, quality metrics other than storage time could aid with inventory management and/or treatment decisions. RBC membrane mechanical fragility is proposed here as one such candidate in vitro metric: it aggregately reflects a range of biochemical and biomechanical changes associated with storage lesion, and can provide a more comprehensive characterization of particular units than other properties. Preliminary data suggest this property can vary substantially among units of equal age, and further work now in progress is investigating its correlation to post-transfusion red cell survival in vivo.

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Mechanical Fragility Calibration of Red Blood Cells

Cited by 28 publications

References 14 publications

Determination of Hemolysis Thresholds by the Use of Data Loggers in Pneumatic Tube Systems

Determination of Hemolysis Thresholds by the Use of Data Loggers in Pneumatic Tube Systems

Stiffness increase of red blood cells during storage

Investigating Direct Non-Age Metrics of Stored Blood Quality Loss

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