Investigating Direct Non-Age Metrics of Stored Blood Quality Loss

Alfano, K.; Tarasev, Michael

doi:10.5580/2789

Cited by 5 publications

(1 citation statement)

References 53 publications

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“…The RBC storage lesion includes several cellular changes associated with a set of metabolic and morphologic alterations in the blood. In retrospective and prospective studies, these changes have been associated with RBC damage, reduced post-transfusion survival/vitality and reduced ability of cells to deliver oxygen to tissues [3,4]. Parameters such as RBC shape, volume, refractive index, and haemoglobin content are important characteristics that can be used as good indicators of the body's physiological state [5].…”

Section: Introductionmentioning

confidence: 99%

White light diffraction phase microscopy for imaging of red blood cells for different storage times

Kocahan,

Çelebioğlu,

Uyanık

2024

Phys. Scr.

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In this study, the effects of different storage times on the surface morphology of red blood cells (RBCs) were investigated using white light diffraction phase microscopy (WDPM). Blood samples collected from 10 volunteer and stored for 56 days, were imaged on WDPM at every 7 days without any sample preparation. To obtain the phase profiles of RCBs, first the sample and then the reference interferograms were obtained from the experimental setup. Then, surface profiles were calculated from these interferogram images using Fourier transform (FT). With the experiment performed every 7 days, 10 RBC phase information were obtained from each sample and surface profiles were created. From these profiles, 7 parameters related with RBC morphology (average cell thickness – ACT; mean corpuscular volume – MCV; projected surface area – PSA; total surface area – SA; diameter – D; mean corpuscular haemoglobin – MCH; surface area to volume ratio - SAV) have been calculated. Therefore, changes in the morphology of RBCs during storage were evaluated quantitatively. Additionally, phase calibration target was used to confirm the accuracy of our experimental setup system. From the interferogram images, the depth of the phase target (GRP 9 and element 3) was obtained as 309 nm, in accordance with the produced depth. By this way, the reliability of the WDPM setup is demonstrated. This study suggests that the morphology of RBCs can be quantitatively obtained in a reliable manner at higher resolution with WDPM without sample preparation.

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Section: Introductionmentioning

confidence: 99%

White light diffraction phase microscopy for imaging of red blood cells for different storage times

Kocahan,

Çelebioğlu,

Uyanık

2024

Phys. Scr.

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Use of Packed Red Blood Cell Mechanical Fragility to Indicate Transfusion Outcomes

Tarasev

Chakraborty²,

Alfano³

et al. 2022

Preprint

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The hypothesis for this study was that RBC mechanical fragility (MF) could be an aggregate in vitro property predictive of transfused RBC performance in vivo. Various MF values were obtained via MF profiling, based on several variations of testing parameters, using both a "legacy" approach (with a commercial, cam-based vertical bead mill and a spectrophotometer) and a more proprietary approach (with a custom-developed, electromagnetic horizontal bead mill combined with proprietary optics and analysis). A total of 52 transfusion events in 32 different patients recruited from the University of Michigan were included in this study. Results were assessed using mixed effects and linear regression models. RBC MF was shown to predict about 15% of transfusion-associated changes in patient hemoglobin concentration, but not of secondary hemolysis-associated metrics (serum hemoglobin, HAP, and LDH). This result was affected by several factors that were not fully accounted for, including variability in post-transfusion blood collection time and variability in each blood unit volumes. Inclusion of the number of units transfused showed the potential to improve predictive capability, thus highlighting the potential importance of underlying patient condition necessitating the second unit transfusion. Certain ways of applying the bead-induced mechanical stress showed MF results more suitable for predicting transfusion outcomes than others indicating potential significance of flow stress type for assessing storage-induced RBC membrane damage. That highlights an opportunity for improvement of the potential for use of MF metrics, through identification of optimal stress application parameters (possibly by further varying parameters used here, as well as others) for assessing contribution of storage-lesion-associated RBC damage on transfused RBC performance.

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Impact of environment on Red Blood Cell ability to withstand mechanical stress

Tarasev¹,

Chakraborty²,

Light

et al. 2016

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Susceptibility of red blood cells (RBC) to hemolysis under mechanical stress is represented by RBC mechanical fragility (MF), with different types or intensities of stress potentially emphasizing different perturbations of RBC membranes. RBC membrane mechanics were shown to depend on cell environment, with many details not yet understood. Here, stress was applied to RBC using a bead mill with oscillation up to 50 Hz, over durations up to 50 minutes. MF profiles plot percent lysis upon stresses of progressive durations. Supplementing media with polyethylene glycol (PEG) which interacts with the cell membrane, but not Dextran which does not, resulted in higher resistance to hemolysis. Albumin, and to a lesser extent fibrinogen and globulins (at physiological concentrations), significantly increased cell ability to withstand mechanical stress versus with un-supplemented buffer solution and with PEG. This is partly due to changes in rheology, per tests done including (PEG) and Dextran, but is mostly due to cell-protein interaction, noting the effect of pH on RBC MF with albumin but not with buffer. Presence of lipids reduced RBC resistance to potentially hemolytic stress with lypemic plasma effecting lower "protection" from induced hemolysis than essentially fatty-acid free plasma. This effect was less dependent on incubation than on fatty-acid presence during stressing. The reduced propensity for hemolysis afforded by plasma proteins also depended markedly on the speed of the bead, potentially reflecting changes from a predominantly Von Karman trail at lower frequencies to an increasingly disorganized turbulent wake at higher frequencies.

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Investigating Direct Non-Age Metrics of Stored Blood Quality Loss

Cited by 5 publications

References 53 publications

White light diffraction phase microscopy for imaging of red blood cells for different storage times

White light diffraction phase microscopy for imaging of red blood cells for different storage times

Use of Packed Red Blood Cell Mechanical Fragility to Indicate Transfusion Outcomes

Impact of environment on Red Blood Cell ability to withstand mechanical stress

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