Measuring synaptic vesicles using cellular electrochemistry and nanoscale molecular imaging

Phan, Nhu T. N.; Li, Xianchan; Ewing, Andrew G.

doi:10.1038/s41570-017-0048

Cited by 210 publications

(167 citation statements)

References 96 publications

Supporting

Mentioning

159

Contrasting

Order By: Relevance

“…During exocytosis, calcium triggers release through SNARE-complex-mediated fusion of vesicles with the cells outer membrane. Consequently, a pore is formed through which the vesicular cargo is released into the extracellular space and is free to interact with a receptor target [1••]. The type of signaling molecule depends on the cell type, for example acetylcholine is released by motor neurons to regulate muscle contraction, insulin is secreted by pancreatic β -cells to regulate the blood glucose level, and norepinephrine, epinephrine and peptides (e.g.…”

Section: Introductionmentioning

confidence: 99%

Vesicle impact electrochemical cytometry compared to amperometric exocytosis measurements

Dunevall

Majdi

Larsson

et al. 2017

Current Opinion in Electrochemistry

View full text Add to dashboard Cite

Three new tools are discussed for understanding chemical communication between cells and primarily to delve into the content and structure of nanometer transmitter vesicles. These are amperometric measurements of exocytosis, vesicle impact electrochemical cytometry, and intracellular vesicle impact electrochemical cytometry. These are combining in the end nanoscale mass spectrometry imaging to begin determination of vesicle structure. These methods have provided solid evidence for the concept of open and closed exocytosis leading to partial release of the vesicle content during normal exocytosis. They have also been used to discover cases where the fraction of transmitter released is not changed, and other cases where the vesicle transmitter fraction released is altered, as with zinc, thought to alter cognition. Overall, the combination of these methods is showing us details of vesicular processes that would not be measureable without these micro and nano electrochemical methods.

show abstract

Section: Introductionmentioning

confidence: 99%

Vesicle impact electrochemical cytometry compared to amperometric exocytosis measurements

Dunevall

Majdi

Larsson

et al. 2017

Current Opinion in Electrochemistry

View full text Add to dashboard Cite

show abstract

“…With ac apability for elemental and isotopic imaging at al ateral spatial resolution of around 50 nm, nanoSIMS has been applied for the quantitation of subcellular protein, [60] DNA, [48] lipid, [61] neurotransmitter [62] and drug [63] distributions in single cells.T og ain additional biological information, the molecular images by nanoSIMS are often correlated with the morphological information obtained by microscopy methods, for example,e lectron microscopy and super-resolution microscopy,a nd with electrochemical measurements. [64] These methods have been thoroughly summarized in recent reviews. [65,66] With high-performance focusing optics that are capable of low micrometer or submicrometer spot sizes,M ALDI-MS allows single-cell and subcellular analysis,r espectively.…”

Section: Angewandte Chemiementioning

confidence: 99%

Single‐Cell Mass Spectrometry Approaches to Explore Cellular Heterogeneity

2018

View full text Add to dashboard Cite

Compositional diversity is a fundamental property in cell populations. Single-cell analysis promises new insight into this cellular heterogeneity on the genomic, transcriptomic, proteomic, and metabolomic levels. Mass spectrometry (MS) is a label-free technique that enables the multiplexed analysis of proteins, peptides, lipids, and metabolites in individual cells. The abundances of these molecular classes are correlated with the physiological states and environmental responses of the cells. In this Minireview, we discuss recent advances in single-cell MS techniques with an emphasis on sampling and ionization methods developed for volume-limited samples. Strategies for sample treatment, separation methods, and data analysis require special considerations for single cells. Ongoing analytical challenges include subcellular heterogeneity, non-normal statistical distributions of cellular properties, and the need for high-throughput, high molecular coverage and minimal perturbation.

show abstract

“…For a conventional electrochemical detection, micro/nanoelectrode is placed outside cells to monitor oxidation currents of secreted vesicles containing dopamine from neuron and neuron‐like cells . By contrast, flame‐etched carbon‐fiber nanotip electrodes have been adapted to monitor these vesicles within cells, enabling the detection of the intracellular catecholamine content of individual nanoscale vesicles in PC12 cells (Figure ) .…”

Section: Electrochemical Intracellular Sensing In Situmentioning

confidence: 99%

Intracellular Electrochemical Sensing

et al. 2018

View full text Add to dashboard Cite

Observing biochemical processes within living cell is imperative for biological and medical research. Fluoresce imaging is widely used for intracellular sensing of cell membranes, nuclei, lysosomes, and pH. Electrochemical assays have been proposed as an alternative to fluorescence‐based assays because of excellent analytical features of electrochemical devices. Notably, thanks to the rapid progress of micro/nanotechnologies and electrochemical techniques, intracellular electrochemical sensing is making rapid progress, leading to a successful detection of intracellular components. Such insight can provide a deep understanding of cellular biological processes and, ultimately, define the human healthy and diseased states. In this review, we present an overview of recent research progress in intracellular electrochemical sensing. We focus on two main topics, electrochemical extraction of cytosolic contents from cells and intracellular electrochemical sensing in situ.

show abstract

Measuring synaptic vesicles using cellular electrochemistry and nanoscale molecular imaging

Cited by 210 publications

References 96 publications

Vesicle impact electrochemical cytometry compared to amperometric exocytosis measurements

Vesicle impact electrochemical cytometry compared to amperometric exocytosis measurements

Single‐Cell Mass Spectrometry Approaches to Explore Cellular Heterogeneity

Intracellular Electrochemical Sensing

Contact Info

Product

Resources

About