Measuring Ca<sup>2+</sup>influxes of TRPC1-dependent Ca<sup>2+</sup>channels in HL-7702 cells with Non-invasive Micro-test Technique

Zhang, Zhenya; Wang, Wenjun; Pan, Longfa; Xu, Yue; Zhang, Zongming

doi:10.3748/wjg.15.4150

Cited by 14 publications

(6 citation statements)

References 31 publications

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“…Root H 2 O 2 effluxes are one of the important ROS-scavenging mechanisms in plants subjected to DS, which were found in citrus plants under DS by using the Non-invasive Micro-test Technique (NMT) 11 . NMT can non-invasively preserve the integrity of the sample and provide dynamic information of specific ions/molecules (including K + , Na + , Ca 2+ , H + , Cl − , Mg 2+ , H 2 O 2 , IAA, and glucose) near material surfaces 21 22 . However, the response of H 2 O 2 effluxes in different order roots and their contribution to root ROS removal are not fully known under DS conditions, as well as under mycorrhization.…”

mentioning

confidence: 99%

Alleviation of drought stress by mycorrhizas is related to increased root H2O2 efflux in trifoliate orange

Huang

Zou

2017

Sci Rep

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The Non-invasive Micro-test Technique (NMT) is used to measure dynamic changes of specific ions/molecules non-invasively, but information about hydrogen peroxide (H2O2) fluxes in different classes of roots by mycorrhiza is scarce in terms of NMT. Effects of Funneliformis mosseae on plant growth, H2O2, superoxide radical (O2·−), malondialdehyde (MDA) concentrations, and H2O2 fluxes in the taproot (TR) and lateral roots (LRs) of trifoliate orange seedlings under well-watered (WW) and drought stress (DS) conditions were studied. DS strongly inhibited mycorrhizal colonization in the TR and LRs, whereas mycorrhizal inoculation significantly promoted plant growth and biomass production. H2O2, O2·−, and MDA concentrations in leaves and roots were dramatically lower in mycorrhizal seedlings than in non-mycorrhizal seedlings under DS. Compared with non-mycorrhizal seedlings, mycorrhizal seedlings had relatively higher net root H2O2 effluxes in the TR and LRs especially under WW, as well as significantly higher total root H2O2 effluxes in the TR and LRs under WW and DS. Total root H2O2 effluxes were significantly positively correlated with root colonization but negatively with root H2O2 and MDA concentrations. It suggested that mycorrhizas induces more H2O2 effluxes of the TR and LRs, thus, alleviating oxidative damage of DS in the host plant.

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confidence: 99%

Alleviation of drought stress by mycorrhizas is related to increased root H2O2 efflux in trifoliate orange

Huang

Zou

2017

Sci Rep

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“…Ca 2+ fluxes were calculated by Fick’s law of diffusion: J = −D (dC/dX), where J represents the Ca2 + flux (μmol/cm 2 per second), D is the ion diffusion constant in a particular solution and temperature (cm 2 /s), dC is the Ca 2+ concentration difference (1 × 10 –3 mol/L), and dX is the 10-μm excursion over which the electrode moved in the detection. Data and image acquisition, preliminary processing, control of the three-dimensional electrode positioner, and stepper motor-controlled fine focus of the microscope stage were performed using ASET software ( Zhang Z. Y. et al, 2009 ; Li et al, 2012 ).…”

Section: Methodsmentioning

confidence: 99%

miR-212/132-Enriched Extracellular Vesicles Promote Differentiation of Induced Pluripotent Stem Cells Into Pancreatic Beta Cells

Bai

Qiwei

Liu

et al. 2021

Front. Cell Dev. Biol.

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Pancreatic beta cell transplantation is the ideal method for treatment of type 1 diabetes mellitus (T1DM), and the generation of beta cells from induced pluripotent stem cells (iPSCs) of patients is a promising strategy. In this study, we improved a previous strategy to produce beta cells using extracellular vesicles (EVs) derived from mature beta cells and differentiated beta cells from iPSCs (i-Beta cells), which secreted insulin under glucose stimulation in vitro and ameliorated hyperglycemia in vivo. Mechanistic analyses revealed that EV-carried microRNA (miR)-212/132 (EV-miR-212/132) directly bound to the 3′ UTR of FBW7 to prevent its translation and FBW7 combined with NGN3 to accelerate its proteasomal degradation. EV-miR-212/132 stabilized NGN3 expression to promote differentiation of endocrine cells from induced iPSCs. Moreover, NGN3 bound to PDX1 to enhance transcription of endogenous miR-212/132 and formed a positive regulatory circuit that maintained the functions of mature pancreatic beta cells.ConclusionThis study describes a novel approach for beta cell production and supports the use of iPSCs for cell replacement therapy of T1DM.

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“…Non-invasive micro-test (NMT) technology was applied to measure net Ca 2+ flux near the plasma membrane of HUVECs using the NMT100 Series (Younger USA LLC, Amherst, MA, USA) equipped with Ca 2+ -sensitive microelectrodes. This technique allows to non-invasively obtain the dynamic information of ionic or molecular activities on material surfaces [46]. Briefly, HUVECs were treated with the same way as that for intracellular calcium imaging.…”

Section: Non-invasive Micro-test To Measure Transmembrane Ca 2+ Fluxmentioning

confidence: 99%

Silica nanomaterials induce organ injuries by Ca2+-ROS-initiated disruption of the endothelial barrier and triggering intravascular coagulation

Wang

Zhao

et al. 2020

Part Fibre Toxicol

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Background: The growing use of silica nanoparticles (SiNPs) in many fields raises human toxicity concerns. We studied the toxicity of SiNP-20 (particle diameter 20 nm) and SiNP-100 (100 nm) and the underlying mechanisms with a focus on the endothelium both in vitro and in vivo. Methods:The study was conducted in cultured human umbilical vein endothelial cells (HUVECs) and adult female Balb/c mice using several techniques.Results: In vitro, both SiNP-20 and SiNP-100 decreased the viability and damaged the plasma membrane of cultured HUVECs. The nanoparticles also inhibited HUVECs migration and tube formation in a concentrationdependent manner. Both SiNPs induced significant calcium mobilization and generation of reactive oxygen species (ROS), increased the phosphorylation of vascular endothelial (VE)-cadherin at the site of tyrosine 731 residue (pY731-VEC), decreased the expression of VE-cadherin expression, disrupted the junctional VE-cadherin continuity and induced F-actin re-assembly in HUVECs. The injuries were reversed by blocking Ca 2+ release activated Ca 2+ (CRAC) channels with YM58483 or by eliminating ROS with N-acetyl cysteine (NAC). In vivo, both SiNP-20 and SiNP-100 (i.v.) induced multiple organ injuries of Balb/c mice in a dose (range 7-35 mg/kg), particle size, and exposure time (4-72 h)-dependent manner. Heart injuries included coronary endothelial damage, erythrocyte adhesion to coronary intima and coronary coagulation. Abdominal aorta injury exhibited intimal neoplasm formation. Lung injuries were smaller pulmonary vein coagulation, bronchiolar epithelial edema and lumen oozing and narrowing. Liver injuries included multifocal necrosis and smaller hepatic vein congestion and coagulation. Kidney injuries involved glomerular congestion and swelling. Macrophage infiltration occurred in all of the observed organ tissues after SiNPs exposure. SiNPs also decreased VE-cadherin expression and altered VE-cadherin spatial distribution in multiple organ tissues in vivo. The largest SiNP (SiNP-100) and longest exposure time exerted the greatest toxicity both in vitro and in vivo.

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Measuring Ca²⁺influxes of TRPC1-dependent Ca²⁺channels in HL-7702 cells with Non-invasive Micro-test Technique

Cited by 14 publications

References 31 publications

Alleviation of drought stress by mycorrhizas is related to increased root H2O2 efflux in trifoliate orange

Alleviation of drought stress by mycorrhizas is related to increased root H2O2 efflux in trifoliate orange

miR-212/132-Enriched Extracellular Vesicles Promote Differentiation of Induced Pluripotent Stem Cells Into Pancreatic Beta Cells

Silica nanomaterials induce organ injuries by Ca2+-ROS-initiated disruption of the endothelial barrier and triggering intravascular coagulation

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Measuring Ca2+influxes of TRPC1-dependent Ca2+channels in HL-7702 cells with Non-invasive Micro-test Technique

Cited by 14 publications

References 31 publications

Alleviation of drought stress by mycorrhizas is related to increased root H2O2 efflux in trifoliate orange

Alleviation of drought stress by mycorrhizas is related to increased root H2O2 efflux in trifoliate orange

miR-212/132-Enriched Extracellular Vesicles Promote Differentiation of Induced Pluripotent Stem Cells Into Pancreatic Beta Cells

Silica nanomaterials induce organ injuries by Ca2+-ROS-initiated disruption of the endothelial barrier and triggering intravascular coagulation

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Measuring Ca²⁺influxes of TRPC1-dependent Ca²⁺channels in HL-7702 cells with Non-invasive Micro-test Technique