Measurement of Superoxide Dismutase-like Activity of Natural Antioxidants

Kim, Seok Joong; Han, Dongil; Moon, Kwang Deog; Rhee, Jae-Sung

doi:10.1271/bbb.59.822

Cited by 103 publications

(46 citation statements)

References 3 publications

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“…Rice bran oil is a rich source of γ-oryzanol (γ-ORZ), which contains a number of phytosteryl ferulates such as 24-methylenecycloartanyl ferulate (24-mCAF), cycloartenyl ferulate (CAF), campesteryl ferulate, β-sitosteryl ferulate (β-SF), and campestanyl ferulate; and the mixture of these is called as γ-ORZ (14). It is of particular importance that γ-ORZ exhibits antioxidant properties in in vitro systems (15). The antioxidant potency of γ-ORZ makes it a good candidate for pharmaceutical drugs, cosmetic formulations, and health food (16).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant, Free Radical–Scavenging, and NF-κB–Inhibitory Activities of Phytosteryl Ferulates: Structure–Activity Studies

et al. 2009

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Abstract. Some of the pharmacological properties of phytosteryl ferulates may be linked to their antioxidant potential. In this study, 2,2-diphenyl-1-picrylhydrazyl (DPPH), electron spin resonance (ESR), and thiobarbituric acid-reactive substances (TBARS) assays demonstrated that phytosteryl ferulates such as cycloartenyl ferulate (CAF), 24-methylenecycloartanyl ferulate (24-mCAF), and β-sitosteryl ferulate (β-SF) and ferulic acid (FA) each exerted strong free radical scavenging and antioxidation of lipid membrane, which were comparable to α-tocopherol. However, the sterol moiety alone, such as cycloartenol (CA), had neither activity. Since, the reactive oxygen species (ROS) production in the cell complex defense mechanism cannot be ruled out with the cell free system, we measured ROS production in NIH 3T3 fibroblast cells induced by H 2 O 2 . CAF and ethyl ferulate (eFA) greatly decreased the ROS level in this system. CA also significantly inhibited the ROS level, suggesting that CA could inhibit ROS production in living cells. Besides these, CAF, 24-mCAF, β-SF, as well as eFA and CA, all these chemicals significantly inhibited the NF-κB activity as analyzed by measuring translocation of NF-κB p65 in LPS-stimulated RAW 264.7 macrophages. These observations revealed that phytosteryl ferulates are responsible for the antioxidant and anti-inflammatory activity via ROS scavenging and inhibition of ROS production.

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Section: Introductionmentioning

confidence: 99%

Antioxidant, Free Radical–Scavenging, and NF-κB–Inhibitory Activities of Phytosteryl Ferulates: Structure–Activity Studies

et al. 2009

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“…Drosophila was used as the model organism, and SOS antioxidants were chosen from the water-soluble types studied in our previous work. 13 ) …”

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confidence: 99%

Effect of Glutathione, Catechin, and Epicatechin on the Survival ofDrosophila melanogasterunder Paraquat Treatment

Kim

Han

Ahn

et al. 1997

Bioscience, Biotechnology, and Biochemistry

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“…The superoxide radical scavenging activity was analyzed according to the procedure of Kim with some modifications (Kim et al, 1995). The reaction system contained 0.4 mL of 25 mM pyrogallol solution, 1 mL of various concentrations of sample (0.25, 0.5, 1.0, 2.5, and 5 mg/mL of COS), and 4.5 mL of 50 mM Tris-HCl buffer (pH 8.2).…”

Section: Methodsmentioning

confidence: 99%

Investigation of the antioxidant activity of chitooligosaccharides on mice with high-fat diet

Han

2016

R. Bras. Zootec.

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-The objective of this study was to analyze the antioxidant activities of chitooligosaccharides (COS) both in vitro and in high-fat diet (HFD)-mouse model. In antioxidant assays in HFD-mouse model, mice were administered with normal diet, HFD, or HFD with 0.5% COS for six weeks. The administration of HFD with 0.5% COS resulted in significant increase in the activity of superoxide dismutase, catalase, and glutathione peroxidase in stomach, liver, and serum of mice as compared with the HFD-fed group, which means that COS may have certain antioxidant activity and can restore the activity of the enzymes affected by the HFD. Through morphological measurements of the small intestinal mucosa, mice fed HFD showed decreased villus height compared with other groups. On the other hand, HFD with 0.5% COS group showed similar ratio of villus height to depth compared with control mice, indicating that intestinal integrity was improved when COS was added. Chitooligosaccharides have potent antioxidant activity that can protect mice from oxidative stress.

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Measurement of Superoxide Dismutase-like Activity of Natural Antioxidants

Cited by 103 publications

References 3 publications

Antioxidant, Free Radical–Scavenging, and NF-κB–Inhibitory Activities of Phytosteryl Ferulates: Structure–Activity Studies

Antioxidant, Free Radical–Scavenging, and NF-κB–Inhibitory Activities of Phytosteryl Ferulates: Structure–Activity Studies

Effect of Glutathione, Catechin, and Epicatechin on the Survival ofDrosophila melanogasterunder Paraquat Treatment

Investigation of the antioxidant activity of chitooligosaccharides on mice with high-fat diet

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