Effect of Glutathione, Catechin, and Epicatechin on the Survival of<i>Drosophila melanogaster</i>under Paraquat Treatment

Kim, Seok Joong; Han, Dongil; Ahn, Byung-Hak; Rhee, Jae-Sung

doi:10.1271/bbb.61.225

Cited by 21 publications

(13 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As expected, co-supplementation of (1 mM) paraquat and (15 mM) iron was extremely toxic to D. melanogaster [40]. However, the phenolic acid PG and flavanols EGCG efficiently protected Drosophila exposed either to iron alone or in combination with iron and paraquat.…”

Section: Discussionsupporting

confidence: 72%

See 1 more Smart Citation

The Effects of Polyphenols on Survival and Locomotor Activity in Drosophila melanogaster Exposed to Iron and Paraquat

2009

View full text Add to dashboard Cite

Parkinson's disease (PD) is a common progressive neurodegenerative disorder, for which at present no causal treatment is available. On the understanding that the causes of PD are mainly oxidative stress and mitochondrial dysfunction, antioxidants and other drugs are expected to be used. In the present study, we demonstrated for the first time that pure polyphenols such as gallic acid, ferulic acid, caffeic acid, coumaric acid, propyl gallate, epicatechin, epigallocatechin, and epigallocatechin gallate protect, rescue and, most importantly, restore the impaired movement activity (i.e., climbing capability) induced by paraquat in Drosophila melanogaster, a valid model of PD. We also showed for the first time that high concentrations of iron (e.g. 15 mM FeSO(4)) are able to diminish fly survival and movement to a similar extent as (20 mM) paraquat treatment. Moreover, paraquat and iron synergistically affect both survival and locomotor function. Remarkably, propyl gallate and epigallocatechin gallate protected and maintained movement abilities in flies co-treated with paraquat and iron. Our findings indicate that pure polyphenols might be potent neuroprotective agents for the treatment of PD against stressful stimuli.

show abstract

Section: Discussionsupporting

confidence: 72%

“…Specifically, we found that 0.1-1 mM polyphenols protect and rescue Drosophila against 20 mM PQ. A study similar to ours has been performed by Kim and colleagues [40], but with important differences in study design and findings. In that study, wild type isogenic Drosophila Canton S flies (100-150/bottle) were pre-fed with (?…”

Section: Discussionmentioning

confidence: 66%

The Effects of Polyphenols on Survival and Locomotor Activity in Drosophila melanogaster Exposed to Iron and Paraquat

2009

View full text Add to dashboard Cite

show abstract

“…In our experiments, (-)-epicatechin and ibuprofen increased the resistance to oxidative stress of Drosophila melanogaster flies. In other study, the addition of (-)-epicatechin didn't increase the Drosophila resistance to paraquat (Kim et al, 1997). The possible explanation is differences in time of flies' exposure to flavonoid and differences in concentrations of paraquat and (-)-epicatechin.…”

Section: Discussionmentioning

confidence: 86%

Geroprotective and Radioprotective Activity of Quercetin, (-)-Epicatechin, and Ibuprofen in Drosophila melanogaster

et al. 2016

View full text Add to dashboard Cite

The modulation of longevity genes and aging-associated signaling pathways using pharmacological agents is one of the potential ways to prolong the lifespan and increase the vitality of an organism. Phytochemicals flavonoids and non-steroidal anti-inflammatory drugs have a large potential as geroprotectors. The goal of the present study was to investigate the effects of long-term and short-term consumption of quercetin, (-)-epicatechin, and ibuprofen on the lifespan, resistance to stress factors (paraquat, hyperthermia, γ-radiation, and starvation), as well as age-dependent physiological parameters (locomotor activity and fecundity) of Drosophila melanogaster. The long-term treatment with quercetin and (-)-epicatechin didn't change or decreased the lifespan of males and females. In contrast, the short-term treatment with flavonoids had a beneficial effect and stimulated the resistance to paraquat and acute γ-irradiation. The short-term ibuprofen consumption had a positive effect on the lifespan of females when it was carried out at the middle age (30–40 days), and to the survival of flies under conditions of oxidative and genotoxic stresses. However, it didn't change the lifespan of males and females after the treatment during first 10 days of an imago life. Additionally, quercetin, (-)-epicatechin, and ibuprofen decreased the spontaneous locomotor activity of males, but had no effect of stimulated the physical activity and fecundity of females. Revealed quercetin, (-)-epicatechin, and ibuprofen activity can be associated with the stimulation of stress response mechanisms through the activation of pro-longevity pathways, or the induction of hormesis.

show abstract

“…In agreement with others (Long et al , 2009; Peng et al , 2011) and our previous investigations (Jimenez-Del-Rio et al , 2010; Ortega-Arellano et al , 2011), we found that GA prolongs life span and climbing activity in wild type or knocked down D. melanogaster. These data suggest that GA (and probably other polyphenols) may protect DAergic neurons independently of the genetic background of the fly, either via direct interaction with ROS, enzymes, receptors and/or transcription factors (Fraga et al , 2010), or through antioxidant gene regulatory mechanisms (Kim et al , 1997; Peng et al , 2011). Our findings suggest that GA can protect DAergic neurons against PQ.…”

Section: Discussionmentioning

confidence: 97%

Dmp53, basket and drICE gene knockdown and polyphenol gallic acid increase life span and locomotor activity in a Drosophila Parkinson's disease model

2013

View full text Add to dashboard Cite

Understanding the mechanism(s) by which dopaminergic (DAergic) neurons are eroded in Parkinson’s disease (PD) is critical for effective therapeutic strategies. By using the binary tyrosine hydroxylase (TH)-Gal4/UAS-X RNAi Drosophila melanogaster system, we report that Dmp53, basket and drICE gene knockdown in dopaminergic neurons prolong life span (p < 0.05; log-rank test) and locomotor activity (p < 0.05; χ2 test) in D. melanogaster lines chronically exposed to (1 mM) paraquat (PQ, oxidative stress (OS) generator) compared to untreated transgenic fly lines. Likewise, knockdown flies displayed higher climbing performance than control flies. Amazingly, gallic acid (GA) significantly protected DAergic neurons, ameliorated life span, and climbing abilities in knockdown fly lines treated with PQ compared to flies treated with PQ only. Therefore, silencing specific gene(s) involved in neuronal death might constitute an excellent tool to study the response of DAergic neurons to OS stimuli. We propose that a therapy with antioxidants and selectively “switching off” death genes in DAergic neurons could provide a means for pre-clinical PD individuals to significantly ameliorate their disease condition.

show abstract

Effect of Glutathione, Catechin, and Epicatechin on the Survival ofDrosophila melanogasterunder Paraquat Treatment

Cited by 21 publications

References 6 publications

The Effects of Polyphenols on Survival and Locomotor Activity in Drosophila melanogaster Exposed to Iron and Paraquat

The Effects of Polyphenols on Survival and Locomotor Activity in Drosophila melanogaster Exposed to Iron and Paraquat

Geroprotective and Radioprotective Activity of Quercetin, (-)-Epicatechin, and Ibuprofen in Drosophila melanogaster

Dmp53, basket and drICE gene knockdown and polyphenol gallic acid increase life span and locomotor activity in a Drosophila Parkinson's disease model

Contact Info

Product

Resources

About