Measurement of<i>β</i>‐casein in human milk by microparticle‐enhanced nephelometric immunoassay

Cuilliere, M. L.; Molé, Claire; Montagne, P.; Béné, Marie C.; Fauré, G

doi:10.1080/09540109709354949

Cited by 6 publications

(13 citation statements)

References 25 publications

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“…The anti-CN antiserum used was an anti-bovine CN. Its obtention and reactivity against human CN have been previously described and debated (Cuillie`re et al, 1997b). Polyfunctional hydrophilic microparticles of 300$12 nm, synthesized as previously reported (Marchand et al, 1992), were supplied by Sano"-Diagnostics-Pasteur (Marnes, France).…”

Section: Reagentsmentioning

confidence: 99%

Dynamics of the Main Immunologically and Nutritionally Available Proteins of Human Milk during Lactation

Montagne¹,

Cuillière²,

Molé³

et al. 2000

Journal of Food Composition and Analysis

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Section: Reagentsmentioning

confidence: 99%

Dynamics of the Main Immunologically and Nutritionally Available Proteins of Human Milk during Lactation

Montagne¹,

Cuillière²,

Molé³

et al. 2000

Journal of Food Composition and Analysis

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“…Beta-casein (β-CN) is the major casein of human milk (1). The main characteristics and functions of β-CN have been previously described (2). With κ-casein (κ-CN), present in milk at a lower concentration, β-CN forms micelles that provide amino acids, calcium, and phosphorus to the developing infant (3).…”

Section: Introductionmentioning

confidence: 99%

“…Changes in both β-CN and κ-CN levels in human milk during lactation have not been much described, even if several methods (FPLC, polyacrylamide gel electrophoresis, isoelectric precipitation, or electroimmunodiffusion) have been proposed for the quantitation of these caseins, especially for β-CN (2,14,15). Microparticle-enhanced nephelometric immunoassays have been previously described as sensitive and accurate techniques for the determination of various human serum proteins (16) and of the main components of bovine milk, especially κ-CN (17) and β-CN (18).…”

Section: Introductionmentioning

confidence: 99%

“…These immunoassays are based on the nephelometric quantitation of the inhibition of microparticle-antigen conjugate immunoagglutination by the antigen to be assayed (19). In addition to the assay previously reported for β-CN in human milk (2), the present work describes the development of a microparticle-enhanced nephelometric immunoassay of κ-CN and their application to the exhaustive investigation of quantitative changes in hu-man milk κ-CN and β-CN during the first twelve weeks of lactation.…”

Section: Introductionmentioning

confidence: 99%

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Changes in the ?-casein and ?-casein concentrations in human milk during lactation

Cuilli�re¹,

Tr�goat²,

B�n�³

et al. 1999

J. Clin. Lab. Anal.

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A microparticle-enhanced nephelometric immunoassay was developed for κ-casein quantification in human milk. Together with a previously reported β-casein comparable immunoassay, it was applied to 862 samples milk, collected from 82 mothers, to investigate the changes in casein concentrations in human milk during the first twelve weeks of lactation. κ-casein immunoassay is sensitive (detection limit in the reaction mixture, 0.02 mg/L) and can be performed in diluted milk, excluding any interference or sample pretreatment. It allowed the quantitation of κ-casein over a large range of concentrations (0.14-4.56 g/L) with accuracy and precision (coefficients of variation from 3 to 10%).βand κ-casein concentrations and percentages among milk total proteins increase between colostrum (2.6 g/L, 14.3% and 1.2 g/L, 6.5%, respectively) and transitional milk (4.4 g/L, 33.2% and 1.3 g/L, 9.5%), decrease at different rates from the third to the eighth week, then remain stable at least up to the end of the third month of lactation (2.7 g/L, 25.3% and 0.9 g/L, 8.5%). The β-casein/κ-casein ratio is higher in colostrum (0.61) than in transitional and mature milk (0.30) and could be related to a better digestibility of colostrum casein micelles by the neonate during the first days of life.

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“…Anti-␤-CN and anti--CN antisera were antibovine CN. Their obtention, specificity, and reactivity against human CN have been previously described and debated (16,17).…”

Section: Reagentsmentioning

confidence: 99%

Measurement of nine human milk proteins by nephelometric immunoassays: application to the determination of mature milk protein profile

Montagne¹,

Trégoat²,

Cuillière³

et al. 2000

Clinical Biochemistry

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Objectives: Microparticle-enhanced nephelometric immunoassays for six human milk proteins (␤-casein, -casein, ␣-lactalbumin, serum albumin, lactoferrin, and lysozyme) and conventional immunonephelometry assays for immunoglobulin A, C3, and C4 complement proteins were developed and characterized. Design and methods: Microparticle-enhanced nephelometric immunoassays are competitive assays based on the nephelometric quantification of the inhibition of microparticle-protein conjugates immunoagglutination by the proteins to be assayed. Results: High precision (CVs ranged from 1% to 14% in within-and between-assays) and recovery (linear recovery in dilution-overloading assay) ensure a reliable determination of the main human milk proteins by single-step homogeneous nephelometric immunoassays, accurate over wide ranges of concentration. These immunoassays were easily applied to a large number of mature human milk samples (between 373 and 503 according to the proteins tested). Conclusions:The immunoassays developed could be applied to the fast determination of human milk protein profile usable for nursery milk bank and fortification.

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Measurement ofβ‐casein in human milk by microparticle‐enhanced nephelometric immunoassay

Cited by 6 publications

References 25 publications

Dynamics of the Main Immunologically and Nutritionally Available Proteins of Human Milk during Lactation

Dynamics of the Main Immunologically and Nutritionally Available Proteins of Human Milk during Lactation

Changes in the ?-casein and ?-casein concentrations in human milk during lactation

Measurement of nine human milk proteins by nephelometric immunoassays: application to the determination of mature milk protein profile

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