Measurement of nine human milk proteins by nephelometric immunoassays: application to the determination of mature milk protein profile

Montagne, P.; Trégoat, Virginie; Cuillière, Marie Louise; Béné, Marie C.; Fauré, G

doi:10.1016/s0009-9120(00)00059-x

Cited by 20 publications

(6 citation statements)

References 26 publications

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“…Conventional methods including radial immunodiffusion, immunoelectrophoresis, enzyme-linked immunosorbent assay (ELISA), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and microparticle-enhanced nephelometric immunoassay were the commonly used analytical methods to quantitate individual proteins in human milk [6, 28, 29, 67–69]. Previous studies have shown similar concentrations of these proteins using other techniques [65, 67–69].…”

Section: Resultsmentioning

confidence: 99%

Quantitation of human milk proteins and their glycoforms using multiple reaction monitoring (MRM)

et al. 2016

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Human milk plays a substantial role in the child growth, development and determines their nutritional and health status. Despite the importance of the proteins and glycoproteins in human milk, very little quantitative information especially on their site-specific glycosylation is known. As more functions of milk proteins and other components continue to emerge, their fine-detailed quantitative information is becoming a key factor in milk research efforts. The present work utilizes a sensitive label-free MRM method to quantify seven milk proteins (α-lactalbumin, lactoferrin, secretory immunoglobulin A, immunoglobulin G, immunoglobulin M, α1-antitrypsin, and lysozyme) using their unique peptides while at the same time, quantifying their site-specific N-glycosylation relative to the protein abundance. The method is highly reproducible, has low limit of quantitation, and accounts for differences in glycosylation due to variations in protein amounts. The method described here expands our knowledge about human milk proteins and provides vital details that could be used in monitoring the health of the infant and even the mother.

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Section: Resultsmentioning

confidence: 99%

Quantitation of human milk proteins and their glycoforms using multiple reaction monitoring (MRM)

et al. 2016

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“…In the case of LZ, it was observed that values were highly variable between donors (0·1–14·51 mg/l). LZ has been reported to be one of the human milk proteins with the highest variability in concentration (Montagne et al 2000). This could be due to individual differences, but factors like age and infections in the mammary gland could also influence it greatly (Emmett & Rogers, 1997; Ferranti et al 2004).…”

Section: Resultsmentioning

confidence: 99%

Activity againstListeria monocytogenesof human milk during lactation. A preliminary study

López-Expósito

Manso

López‐Fandiño

et al. 2008

Journal of Dairy Research

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Human milk samples from three healthy donors were investigated in order to evaluate the antibacterial activity during lactation against Escherichia coli ATCC 25922 and Listeria monocytogenes. The concentration of the main human-milk antimicrobial proteins (lactoferrin (LF), lysozyme (LZ) and secretory immunoglobulin A (sIgA)) was determined by ELISA. Results showed that human milk exhibited antibacterial activity against List. monocytogenes, although it was weakly active against Esch. coli ATCC 25922. The observed antilisterial activity was positively correlated with LZ concentration. In addition, the effect of gastrointestinal proteases, at different pH conditions, that prevail in the stomach of infants (pH 2.0-6.5), on antilisterial activity and protein degradation was evaluated. Hydrolysis with pepsin at pH 4.0-6.5, followed by treatment with pancreatic enzymes, resulted in a decreased hydrolysis of LZ, LF and sIgA and an enhanced antibacterial activity against List. monocytogenes. It is suggested that partial degradation of certain milk proteins at the gastrointestinal level may produce peptides that could act synergistically with the remnant intact proteins.

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“…The human milk-fed preterm infant may experience improved health, such as, lower rate of infection, necrotizing enterocolitis, better gastrointestinal function, and neurodevelopment [24, 25]. The effects of milk cytokines on the maturation and functions of the epithelium, mucosal, or submucosal leukocytes and other specialized cells and structures in the alimentary and respiratory system should be particularly targeted for investigation [8].…”

Section: Discussionmentioning

confidence: 99%

Levels of Cytokines (IL‐1β, IL‐2, IL‐6, IL‐8, TNF‐α) and Trace Elements (Zn, Cu) in Breast Milk From Mothers of Preterm and Term Infants

Üstündağ

Yı́lmaz

Doğan

et al. 2005

Mediators of Inflammation

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It has been well documented that human milk contains several immunomodulator components which are important during infant period when the newborn's immune system is still under development. In this study, we aim at examining levels of cytokines, zinc (Zn), and copper (Cu) in milk from mothers of premature and mature infants, and comparing changes during lactation periods consequently. Milk was collected from total of 40 mothers (group M: mothers of mature infants, n = 20; group PM: mothers of premature infants, n = 20) from four lactation stages: colostrum (0–7 days), transitional (7–14 days), mature milk (21 days), and mature milk (2nd month). Levels of cytokines (interleukin [IL]-lβ, IL-2, IL-6, IL-8, tumor necrosis factor-alpha [TNF-α]) were determined by chemiluminesence method, whereas atomic absorption spectrophotometer was used for the determination of Zn and Cu levels. Cytokine levels were determined to be high in colostrum and transient milk from mothers of full-term infants, whereas their levels were reduced drastically in the 21st day and the 2nd month milk (P < .01 , P < .001). Similar trends were observed in milk from mothers of premature infants, but cytokine levels were significantly lower in colostrum compared to colostrum from mothers of mature infants (P < .01). The differences in cytokine levels were continuous in transient milk (P < .05) and mature milk (21 days) (P < .05), whereas there was no statistically significant differences between milk from both groups of mothers in the 2nd month (P > .05). Zn levels in milk from mothers of premature infants were significantly lower compared to the ones from mothers of mature infants (P < .01) and these differences continued through the 2nd month. Although Cu levels were lower in milk from mothers of premature infants, there was no statistically significant difference except colostrum (P > .05). Our results clearly demonstrate that the level of immunomodulating agents such as cytokines and trace elements in milk from mothers of premature infants is less than the level of the same agents in milk from mothers of full-term infants. Although there are commercially available products for infant feeding, human milk is still the best natural nutrient for newborns. Therefore, when premature infants are breastfed, necessary precautions such as supplemantary diets must be considered for possible infections and risks related with immune system deficiency.

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Measurement of nine human milk proteins by nephelometric immunoassays: application to the determination of mature milk protein profile

Cited by 20 publications

References 26 publications

Quantitation of human milk proteins and their glycoforms using multiple reaction monitoring (MRM)

Quantitation of human milk proteins and their glycoforms using multiple reaction monitoring (MRM)

Activity againstListeria monocytogenesof human milk during lactation. A preliminary study

Levels of Cytokines (IL‐1β, IL‐2, IL‐6, IL‐8, TNF‐α) and Trace Elements (Zn, Cu) in Breast Milk From Mothers of Preterm and Term Infants

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