Measurement of hepatic blood flow by colloidal gold clearance

Razzak, Mohamed A.; Wagner, Henry N.

doi:10.1152/jappl.1961.16.6.1133

Cited by 9 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since our results followed the mathematical model suggested first by Michaelis and Menten in their studies of enzyme-substrate systems (2), it was possible to postulate that the rate of removal of small amounts of particulate matter from the blood is determined chiefly by the frequency of collision of the particles with RE cells. This concept is also consistent with the known dependence of the clearance rate of trace quantities of particles on the blood flow to the principal RES-containing organs, chiefly the liver and spleen (3). As the number of particles increases, however, the collision frequency is no longer a factor in the reaction velocity, the latter being governed only by the rate of the reaction of the particles with the RE cells.…”

supporting

confidence: 81%

Studies of the Reticuloendothelial System (Res). Ii. Changes in the Phagocytic Capacity of the Res in Patients With Certain Infections*

Wagner¹,

Iio²,

Hornick³

1963

J. Clin. Invest.

Self Cite

View full text Add to dashboard Cite

In our previous communication (1), we have presented data indicating that the phagocytic capacity of the reticuloendothelial system (RES) can be determined by measuring the rate of disappearance of various doses of radioisotopically labeled aggregated albumin particles from the circulation. Since our results followed the mathematical model suggested first by Michaelis and Menten in their studies of enzyme-substrate systems (2), it was possible to postulate that the rate of removal of small amounts of particulate matter from the blood is determined chiefly by the frequency of collision of the particles with RE cells. This concept is also consistent with the known dependence of the clearance rate of trace quantities of particles on the blood flow to the principal RES-containing organs, chiefly the liver and spleen (3). As the number of particles increases, however, the collision frequency is no longer a factor in the reaction velocity, the latter being governed only by the rate of the reaction of the particles with the RE cells. This is evidenced by the fact that progressively larger doses do not increase the rate of removal of the particles to a significant degree.The details of the method and the analytical procedure are discussed in the previous paper (1). The purpose of this communication is to describe the effects of certain infections, namely, pneumococcal pneumonia, typhoid fever, sandfly fever, and tularemia, on the phagocytic capacity of the reticuloendothelial system in man.

show abstract

supporting

confidence: 81%

Studies of the Reticuloendothelial System (Res). Ii. Changes in the Phagocytic Capacity of the Res in Patients With Certain Infections*

Wagner¹,

Iio²,

Hornick³

1963

J. Clin. Invest.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The technique of using the clearance of a colloid to determine hepatic blood flow has been employed frequently (Dobson & Jones, 1952;Benacerraff et al, 1957;Biozzi et al, 1958;Razzak & Wagner, 1961).…”

Section: Determination Ofhepatic Bloodflowmentioning

confidence: 99%

Mechanism of increased hepatic uptake of unesterified fatty acid from serum of ethanol-treated rats

Abrams

Cooper

1976

Biochemical Journal

View full text Add to dashboard Cite

Studies were made on the mechanism by which livers of ethanol-treated rats take up an increased fraction of the total flux of unesterified fatty acid in serum. It was found that ethanol (0.7g/kg) causes a twofold rise in the serum content of liver, and that this serum is in rapid equilibrium with the general circulation. The fractional hepatic uptake from serum of group of compounds with varying uptake mechanisms and metabolic fates was studied in control and ethanol-treated animals. All the compounds tested, including unesterified fatty acid, showed an enhanced uptake when ethanol was given. For one of the compounds, carbon tetrachloride, a dose/response relationship was established between the amount administered, the amount taken up by liver, and the amount metabolized. These findings were interpreted to mean that this dose of ethanol causes the liver to receive an increased flow of blood, and as a result all compounds present and capable of being taken by liver are taken up at an increased rate. Hepatic blood flow was measured by a technique that monitors the rate of clearance of a colloidal lipid emulsion. It was found that ethanol increased hepatic blood flow by about 60%. This effect of ethanol on hepatic blood flow provides an explanation for the fatty liver and the synergistic effect between an acute dose of ethanol and carbon tetrachloride. A hypothesis to explain why a moderate dose of ethanol causes triglyceride to accumulate in liver is presented.

show abstract