Mechanism of increased hepatic uptake of unesterified fatty acid from serum of ethanol-treated rats

Abrams, M A; Cooper, Cecil

doi:10.1042/bj1560047

Cited by 38 publications

(9 citation statements)

References 21 publications

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“…The concentration of unesterified fatty acid in liver was also measured and this very small, rapidly turning-over pool (0.036mg/g of liver) remained unchanged. This cannot be accounted for by the presence of blood in the isolated liver because we measured this parameter (Abrams & Cooper, 1976) as well as the concentration ofunesterified fatty acid in serum. By 4h the concentration of hepatic triglyceride began to decline.…”

Section: Resultsmentioning

confidence: 99%

“…The fractions are constant and measure the percentage of total flux of unesterified fatty acid through serum that is attributable to liver. Although ethanol has no effect on total flux of unesterified fatty acid through serum (Table 3) present in liver after its removal from the animal (Abrams & Cooper, 1976).…”

Section: Fraction Offlux Ofunesterifiedfatty Acidin Serum Going To Thmentioning

confidence: 95%

“…Each point is the meanf±s.E.M., obtained with five to ten rats. All values are corrected for residual blood in liver (Abrams & Cooper, 1976). 0, Ethanol-treated; o, control.…”

Section: Effect Ofdose Ofethanol Onphysiologicalparametersmentioning

confidence: 99%

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Quantitative analysis of metabolism of hepatic triglyceride in ethanol-treated rats

Abrams

Cooper

1976

Biochemical Journal

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An acute intraperitoneal injection of ethanol (0.7 or 2.1g/kg body wt.) causes the reversible, dose-dependent accumulation of hepatic triglyceride in rats. By using a pulse of [14C]palmitate injected into a tail vein, it was found that ethanol (2.1g/kg)had no effect on the flux of unesterified fatty acid of serum (4.3mumol/min per 100g body wt.). However, either dose increased the fraction of the total flux going to liver from 0.16 to0.27 as rapidly as could be measured (30s), and it remained elevated until all ethanol had been cleared from the blood. The fraction of the total radioactivity in lipids of liver that was in triglyceride increased linearly for 1 h from 30 to 50% and there was a simultaneous decrease in phospholipid from 60 to 40%. The rate of synthesis of hepatic triglyceride derived directly from unesterified fatty acid of serum was calculated by using the flux rate of unesterified fatty acid in serum, the fractional hepatic uptake of this flux, and the percentage of liver fatty acid esterified to triglyceride. This contribution is related to the total synthetic rate of hepatic triglyceride (rate of accumulation+rate of release) to determine quantitatively how much of the developing fatty liver is attributable to increased uptake of unesterfied fatty acid of serum. At the higher dose of ethanol, about half of the accumulating triglyceride is derived from this source, whereas with the lower dose of ethanol it can account for all of the build-up.

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Section: Resultsmentioning

confidence: 99%

Section: Fraction Offlux Ofunesterifiedfatty Acidin Serum Going To Thmentioning

confidence: 95%

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Quantitative analysis of metabolism of hepatic triglyceride in ethanol-treated rats

Abrams

Cooper

1976

Biochemical Journal

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“…A reserve for extraction therefore exists which could overcome a hepatocellular dysfunction so that two low doses of ICG (0.26 pmol/kg, 0.13 pmol/kg in our study) should give the same t112 (i.e. the plotted curves should be parallel) [19]. When these conditions are obtained, a low HER indicates the presence of non-functional intrahepatic shunts.…”

Section: Fhbf=x Bv;mentioning

confidence: 73%

Hepatic vein oxygenation, liver blood flow, and the rate of ethanol metabolism in recently abstinent alcoholic patients

Iturriaga

Ugarte²,

Israel

1980

Eur J Clin Investigation

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To determine whether hepatic hypoxia is associated with hepatocellular necrosis in alcoholics, oxygen tension in the hepatic vein and hepatic blood flow were determined in thirteen patients without overt clinical liver disease. Ethanol metabolic rate was also assayed as an index of liver metabolism. Hepatic blood flow and ethanol metabolic rate were also determined in six normal volunteers. According to liver histology patients were separated into two groups, with and without hepatocellular necrosis. Alcoholics with necrosis showed a higher (P less than 0.002) ethanol metabolic rate (4.05 +/- 0.23 mmol/kg/h) than those without necrosis (2.46 +/- 0.34). Hepatic blood flow in the total group of alcoholics was not significantly different from controls; in the group with necrosis it was lower (651.7 +/- 44.6 ml/min/m2) than in the group without necrosis (878.3 +/- 81.6; P less than 0.025). Hepatic vein pO2 was lower (P less than 0.01) in patients with hepatocellular necrosis (31.7 +/- 0.68 mmHg) than in patients without necrosis (35.7 +/- 0.99). In the whole group, a significant negative correlation (r = 0.76, P less than 0.003) was observed between hepatic vein pO2 and ethanol metabolic rate. Acute administration of ethanol (21.7 mmol/kg) did not alter hepatic blood flow in six normal individuals nor in five alcoholic patients, although an increase in hepatic vein pO2 was observed in the latter. The changes observed in hepatic vein pO2 functional hepatic blood flow, and ethanol metabolic rate which correlate with hepatocellular necrosis, may be of pathogenic importance in alcoholic liver disease.

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“…Feeding allicin (garlic) to rats (100 mg / (kg day) for 15 days enhanced the activity of liver lipase and lowered glucose-6-phosphatase activity (Augusti and Mathew, 1975). The activity of serum and liver enzymes was inhibited by garlic extracts (Abrams and Cooper, 1976). In this study, rats fed on the olive or flaxseed oil diets+ 2%garlic or onion powders have a marked elevation in the most liver mineral concentrations, while reverse observations were found in the most tibial mineral concentrations, similar to other previous report (Chatty et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Effect of dietary garlic and onion on liver and tibial mineral concentrations in omega-3 fatty acids rich oil fed rats

Mohamed¹,

Kader²

2011

ABJNA

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This study was designed to compare the effect of garlic or onion (2%) on mineral concentrations in liver and tibia of rats fed with diet rich in omega-3 fatty acids. Experiments were carried out on Male Sprague-Dawley rats fed AIN93 semi-purified diets containing either 8 % flaxseed or olive oils replacing corn oil with or without garlic or onion (2%) for 28 days. The analysis of minerals calcium (Ca), phosphorous (P), potassium (K), magnesium (Mg), sodium (Na), iron (Fe) and zinc (Zn) of liver and tibia were conducted by the Atomic Absorption Spectrophotometer. The results showed significantly increased Zn, Mg and P concentrations in liver and K, Na and P in tibia of rats fed on flaxseed oil diet, compared to rats fed on olive oil diet. While the liver Fe and K as well as tibial Ca and Mg were significantly decreased. The addition of garlic powder (2%) to the olive oil diet produced a marked increase in liver Ca, Zn, K, Mg and P levels .However, there was noticeable reduction in liver Fe and tibial Ca and Na levels. No change in tibial Zn level was found by the addition of either garlic or onion powders (2%) to the olive oil diet. Liver of rats fed on olive oil diet with onion powder (2%) have noticeable elevation in Zn, Mg and P and reduction in Fe and K levels. There was a significant decrease in tibial Ca and Na levels, compared to rats fed on olive oil control diet. Flaxseed oil diet with garlic powders (2%) led to a significant increase in liver P and tibial Mg concentrations. Although a marked reduce was observed in liver Na and tibial Ca, Zn, Na and P levels. The same addition to the same diet caused a non significant decrease in liver Zn and Na as well as tibial Ca, Fe and Zn concentrations. The addition of onion powder (2%) to flaxseed oil diet produced a noticeable increase in liver Fe, k and P levels as well as tibial Mg level. Meanwhile, no marked increase was found in liver Ca, Zn and Na levels and decrease in liver Mg and tibial Fe. A significant decrease was found in tibial Ca, Zn, Na and P concentrations. In conclusion: the present study concluded that adding garlic or onion to diet rich in mono or poly unsaturated fatty acids elevate the levels of some minerals in liver and tibia of rats, Which contributes to the maintenance of the health status of the liver and bone.

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Mechanism of increased hepatic uptake of unesterified fatty acid from serum of ethanol-treated rats

Cited by 38 publications

References 21 publications

Quantitative analysis of metabolism of hepatic triglyceride in ethanol-treated rats

Quantitative analysis of metabolism of hepatic triglyceride in ethanol-treated rats

Hepatic vein oxygenation, liver blood flow, and the rate of ethanol metabolism in recently abstinent alcoholic patients

Effect of dietary garlic and onion on liver and tibial mineral concentrations in omega-3 fatty acids rich oil fed rats

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