Measurement of epithelial thickness within the oral cavity using optical coherence tomography

Prestin, Sven; Rothschild, Sacha I.; Betz, Christian; Kraft, Marcel

doi:10.1002/hed.22007

Cited by 77 publications

(68 citation statements)

References 41 publications

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“…The thickness of the regenerating epithelium in group 1 was measured to be approximately 285 mm. A study was done by Prestin et al 13 to measure the thickness of human buccal epithelium by optical coherence tomography. According to their study, the thickness of human buccal epithelium was 294 mm, which suggests that rat and human buccal epithelium thickness are comparable to each other and that future studies performed on rat buccal mucosa will be of value to researchers.…”

Section: Discussionmentioning

confidence: 99%

Effect of carbonated drinks on wound healing of oral epithelium

Fahim

Ilyas

Jafari³

et al. 2016

Journal of Oral Biology and Craniofacial Research

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Section: Discussionmentioning

confidence: 99%

Effect of carbonated drinks on wound healing of oral epithelium

Fahim

Ilyas

Jafari³

et al. 2016

Journal of Oral Biology and Craniofacial Research

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“…The epithelium in oral mucosa is generally 90 to 300-μm thick. 40 Therefore, a 1.5-mm OCT imaging depth remains capable of imaging the entire epithelium, basement membrane, and into the lamina propria, sufficient for initial assessment of submucosal involvement. The HRF imaging component described here was designed for use with the contrast agent proflavine because of its ability to rapidly stain cell nuclei and, therefore, allow assessment of nuclear morphology, which is a well-established marker of dysplasia and neoplasia.…”

Section: Discussionmentioning

confidence: 99%

Design and characterization of a handheld multimodal imaging device for the assessment of oral epithelial lesions

Higgins

Pierce

2014

J. Biomed. Opt.

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Abstract. A compact handpiece combining high resolution fluorescence (HRF) imaging with optical coherence tomography (OCT) was developed to provide real-time assessment of oral lesions. This multimodal imaging device simultaneously captures coregistered en face images with subcellular detail alongside cross-sectional images of tissue microstructure. The HRF imaging acquires a 712 × 594 μm 2 field-of-view at the sample with a spatial resolution of 3.5 μm. The OCT images were acquired to a depth of 1.5 mm with axial and lateral resolutions of 9.3 and 8.0 μm, respectively. HRF and OCT images are simultaneously displayed at 25 fps. The handheld device was used to image a healthy volunteer, demonstrating the potential for in vivo assessment of the epithelial surface for dysplastic and neoplastic changes at the cellular level, while simultaneously evaluating submucosal involvement. We anticipate potential applications in real-time assessment of oral lesions for improved surveillance and surgical guidance. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…Physiologically, the oral mucosa tissue is generally composed of multiple cell layers; however, for computational speed, the current model assumed six hypothetical sublayers to calculate drug diffusion across the epithelium and lamina propria. Moreover, the thickness fraction (expressed as a percentage) for each sublayer was [27,28] b Surface areas for individual compartments in human adults were obtained from literature [29,30], the surface areas for children can be estimated by scaling adult surface areas based on published information on vocal tract development [31] c Values for epithelium thickness in different regions in humans were obtained from literature [12,22,[32][33][34][35][36][37] d Very limited information for lamina propria thickness is available for humans [32] e pH values in individual regions of the oral cavity for human were found in literature [38] defined by fitting in vitro permeability data in porcine buccal and sublingual tissues. From optimal fitting of the results of intraoral permeability assays for nine model molecules, sublayer 1 represents 5% of the total epithelium thickness, and the remaining 95% of epithelium thickness is split evenly among the remaining five layers.…”

Section: Model Theorymentioning

confidence: 99%

Development of a Novel Oral Cavity Compartmental Absorption and Transit Model for Sublingual Administration: Illustration with Zolpidem

Xia

Yang

Zhou

et al. 2015

AAPS J

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Abstract. Intraoral (IO) delivery is an alternative administration route to deliver a drug substance via the mouth that provides several advantages over conventional oral dosage forms. The purpose of this work was to develop and evaluate a novel, physiologically based oral cavity model for projection and mechanistic analysis of the clinical pharmacokinetics of intraoral formulations. The GastroPlus™ Oral Cavity Compartmental Absorption and Transit (OCCAT™) model was used to simulate the plasma concentration versus time profiles and the fraction and rate of intraoral drug transit/absorption for Intermezzo® sublingual tablets (zolpidem tartrate). The model was evaluated by the goodness-of-fit between simulated and observed concentrations and the deviation of key PK parameters (e.g., C max , T max , and AUC). In addition, a sensitivity analysis was conducted to demonstrate the interplay and impact of key modeling parameters on the fraction absorbed via oral mucosa (F a_IO ). The OCCAT™ model captured the observed pharmacokinetics for Intermezzo® sublingual tablets (R 2 >0.9). The predicted deviations (%) for C max , AUC 0-inf , AUC 0-20 min , and T max were 5.7, 28.0, 11.8, and 28.6%, respectively, indicating good prediction accuracy. The model also estimated~18% of total drug was absorbed via the IO route. Furthermore, the sensitivity analysis indicated that the F a_IO was not only associated with drug diffusivity and unbound fraction in epithelium tissue (f ut ) but also depended on the physicochemical properties of compounds for IO delivery (e.g., solubility and logD pH=7.4 ). The novel physiologically based IO absorption OCCAT™ model showed satisfactory performance and will be helpful to guide development of future intraoral formulations.KEY WORDS: diffusivity; intraoral delivery; oral cavity compartmental absorption and transit (OCCAT™) model; unbound fraction in epithelium tissue; zolpidem.

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Measurement of epithelial thickness within the oral cavity using optical coherence tomography

Abstract: Our data serve as reference values for detecting oral malignancy and determining the approximate grade of dysplasia. In this circumstance, a differentiated view of the different regions is important due to a variation in thickness of the epithelium within the oral cavity.

Cited by 77 publications

References 41 publications

Effect of carbonated drinks on wound healing of oral epithelium

Effect of carbonated drinks on wound healing of oral epithelium

Design and characterization of a handheld multimodal imaging device for the assessment of oral epithelial lesions

Development of a Novel Oral Cavity Compartmental Absorption and Transit Model for Sublingual Administration: Illustration with Zolpidem

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