May nitric oxide molecule have a role in the pathogenesis of human cataract?

Örnek, Kemal; Karel, Fatih; Büyükbingöl, Zeliha

doi:10.1016/s0014-4835(02)00268-3

Cited by 53 publications

(36 citation statements)

References 19 publications

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“…In addition, higher levels of NO end products are found in cataractous lenses of diabetic patients compared to cataractous lenses of healthy individuals [18]. The present study also report high expressions of nitric oxide synthase in cataract samples as com-pared to non cataract samples in senile non-diabetic samples.…”

Section: Discussionsupporting

confidence: 77%

“…However, it was demonstrated that SOD activity levels were significantly increased in diabetic senile cataract group in comparison with the control group [18].…”

Section: Discussionmentioning

confidence: 83%

“…Nitric oxide (NO) generated by nitric oxide synthases (NOS3), and known as a detrimental free radical, play an important role in the various physiological, pathological and aging process [18] [19]. Various biological functions are controlled by NO production which can be served as a second messenger intracellularly and extracellularly as conveyor of information between cells.…”

Section: Introductionmentioning

confidence: 99%

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Biological Association and Expressions of NOS3 & SOD2 in Non-Diabetic Senile Cataractogenesis

Mushtaq¹,

Zehra²,

Khan³

et al. 2016

AER

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Aim: To evaluate the functional relationship between the nitric oxide synthase (NOS) and superoxide dismutase (SOD) enzymes in the pathogenesis of human senile cataract lenses of non-diabetic patients. Methods: Total solubilized proteins from human cataract lens were compared with normal lens (control) by 2-Dimenstional gel electrophoresis (2-DE). Proteins with different abundances were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Western blot analysis was used to verify the changes in expression of NOS3 and SOD2. A further functional association of NOS3 with SOD2 and other proteins was seen by STRING 8.3 databases. Results: In the 2-DE maps, the cataract and normal lens proteins migrated in the region of pH 3 -10 with a relative molecular weight of 20 -130 kDa. Approximately two protein spots with differential intensity were detected as NOS3 and SOD2 using MALDI-TOF-MS. Western blot analysis showed high expression of NOS3 in cataract and SOD2 in normal lens samples. String interaction network revealed strong interactions between NOS3 and SOD2 at high confidence score, which is helpful in characterization of functional abnormalities that may be a causative factor in the pathogenesis of cataract. Conclusion: This study will offer new avenues for mechanistic evaluation and future prevention of cataractogensis. However, large scale studies will be required to evaluate the effect of this interaction on the clinical outcome in human cataract.

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Section: Discussionsupporting

confidence: 77%

“…However, it was demonstrated that SOD activity levels were significantly increased in diabetic senile cataract group in comparison with the control group [18].…”

Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biological Association and Expressions of NOS3 & SOD2 in Non-Diabetic Senile Cataractogenesis

Mushtaq¹,

Zehra²,

Khan³

et al. 2016

AER

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“…In the eye, NO contributes to allergic conjunctivitis, 5 glaucoma, 6 diabetic retinopathy, 7 and also cataract. 8,9 OH·, another highly reactive free radical has been shown to contribute to lens crystalline modification. 10 Fortunately, free radical formation is controlled naturally by various beneficial compounds known as antioxidants.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Lens Aldose Reductase Inhibitory and Free Radical Scavenging Potential of Fractions of Lonchocarpus cyanescens: Potential for Cataract Remediation

Ajani¹,

Sabiu²,

Odufuwa³

et al. 2016

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“…Studies with the analysis of human cataractous lenses for nitrite content have been, so far, inconclusive. 29 The aim of this investigation was, hence, to verify this possibility using organ culture studies with the rat lens in medium containing nitroaspirin [(2-(acetyloxy)-3-[(nitrooxy)-methyl] phenyl ester benzoic acid), Cayman Chemical, Ann Arbor, MI, Catalog No. 10005854], a newly developed NO donor.…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of the Ocular Lens to Nitric Oxide: Implications in Cataractogenesis

Varma

Hegde

2007

Journal of Ocular Pharmacology and Therapeutics

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Oxides of nitrogen, such as nitric oxide (NO), are now biologically referred to as reactive nitrogen species. The generation of NO gives rise to several other reactive species, such as NO+, NO-, NO2, N2O3, and ONOO- and so forth, which are all capable of inflicting tissue damage. Indeed, NO generation is known to be associated with retinal degeneration and glaucoma. Its level has also been found to increase in the aqueous and vitreous humors in diabetes. We hypothesize that such an increase would have a detrimental effect on the biochemistry and metabolism of tissues, including the lens, bathed by the aqueous containing elevated levels of NO. The primary aim of our investigations was, therefore, to examine the susceptibility of the lens to damage by NO in vitro in the presence of nitroaspirin, a novel NO donating agent. The extent of physiologic damage to the lens was initially assessed by determining the integrity of its active transport mechanism. The overall status of tissue metabolism was determined by measuring the adenosine triphosphate (ATP) levels. The levels of glutathione (GSH) and glutathione disulfide, reflecting the status of its antioxidant reserve, were also determined. That NO is indeed deleterious to the lens was apparent by the inhibition of the active transport of Rb(+). This was associated with a substantial decrease in the contents of ATP and GSH, the decrease in the latter directly suggesting that the NO effects are caused by oxidative stress. That the effects are caused by NO generated from nitroaspirin was proven by a substantial increase in NO level in the medium during incubation of the lenses with nitroaspirin, as compared to the controls. The results, therefore, were highly suggestive of a contribution of the oxides of nitrogen in cataract formation associated with diabetes and other aging diseases.

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May nitric oxide molecule have a role in the pathogenesis of human cataract?

Cited by 53 publications

References 19 publications

Biological Association and Expressions of NOS3 & SOD2 in Non-Diabetic Senile Cataractogenesis

Biological Association and Expressions of NOS3 & SOD2 in Non-Diabetic Senile Cataractogenesis

Evaluation of Lens Aldose Reductase Inhibitory and Free Radical Scavenging Potential of Fractions of Lonchocarpus cyanescens: Potential for Cataract Remediation

Susceptibility of the Ocular Lens to Nitric Oxide: Implications in Cataractogenesis

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May nitric oxide molecule have a role in the pathogenesis of human cataract?

Cited by 53 publications

References 19 publications

Biological Association and Expressions of NOS3 &amp; SOD2 in Non-Diabetic Senile Cataractogenesis

Biological Association and Expressions of NOS3 &amp; SOD2 in Non-Diabetic Senile Cataractogenesis

Evaluation of Lens Aldose Reductase Inhibitory and Free Radical Scavenging Potential of Fractions of Lonchocarpus cyanescens: Potential for Cataract Remediation

Susceptibility of the Ocular Lens to Nitric Oxide: Implications in Cataractogenesis

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Biological Association and Expressions of NOS3 & SOD2 in Non-Diabetic Senile Cataractogenesis

Biological Association and Expressions of NOS3 & SOD2 in Non-Diabetic Senile Cataractogenesis