Matrix solid-phase dispersion method for the determination of macrolide antibiotics in sheep’s milk

García-Mayor, M.A.; Gallego-Picó, Alejandrina; Martínez, Rosa María Goig; Fernández-Hernando, P.; Durand-Alegría, J.S.

doi:10.1016/j.foodchem.2012.02.120

Cited by 48 publications

(21 citation statements)

References 22 publications

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“…has been high performance liquid chromatography (HPLC) with tandem mass spectrometric detector [11][12][13][14][15][16][17][18][19]. Aside from the mentioned highly sophisticated detector, diode-array (DAD) [20,21], chemiluminescence [22], fluorescence [23] and electrochemical [3] detectors have also been used. Additionally, spectrophotometric and conductometric methods for the estimation of erythromycin thiocyanate in both pure and pharmaceutical dosage forms were developed [24].…”

Section: Introductionmentioning

confidence: 99%

Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation

Vajdle

Guzsvány

Škorić

et al. 2016

Electrochimica Acta

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Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation, Electrochimica Acta http://dx.2 Graphical abstract 3 Highlights  Voltammetric characterization of erythromycin ethylsuccinate (EES) on Hg(Ag)FE  Trace level determination of EES by electroreduction based SWV and SW-AdSV methods  Protonation of the tertiary amino group supports the adsorption of EES on Hg(Ag)FE  1 H NMR confirms chemical shifting of tertiary amine methyl proton signals with pH  Comparative HPLC-DAD measurements were performed for the validation of the methods 4 AbstractErythromycin, a macrolide antibiotic, has similar antimicrobial spectrum to penicillin and it is widely used, especially in the treatment of patients who are allergic to penicillin.In this work, the application of a renewable silver-amalgam film electrode (Hg (Ag)FE) for the characterization and determination of erythromycin ethylsuccinate (EES), a widely used esterified form of this antibiotic, by means of cyclic voltammetry (CV) and square wave voltammetry (SWV) is presented. In the aqueous Britton-Robinson buffer (pH 5.0-9.0) that served as the supporting electrolyte, one reduction peak of EES was observed in the investigated potential range between -0.75 V and -1.80 V vs SCE, with peak potential maxima ranging from -1.59 V to -1.70 V, which strongly depended on the applied pH, as did the peak shape. For the analytical purposes the pH of 7.0 was selected, since in this electrolyte the EES peak was well-shaped and separated from the background current of the supporting electrolyte in both cases; in the direct cathodic SWV and in the case of square wave adsorptive stripping voltammetry (SW-AdSV). It was established, by the Ep-pH correlation, that protons strongly influenced the electrochemical reduction of EES. The CVs recorded between 0.025 -0.50 V s -1 at pH 7.0 confirmed that the electrode reaction is adsorption-controlled. Based on the series of 1 H NMR measurements it is proved that the tertiary amino group of EES is mainly in its protonated form at pH 7.0 which may lead, at appropriate accumulation potential and time, to the favored adsorption of the target ionic form of the analyte improving on such way the sensitivity of the SW-AdSV method.The optimized procedures resulted in stable SWV responses with good linear correlation in the EES concentration range from 4.53 to 29.8 µg mL -1 (LOD = 1.36 µg mL -1 ), and from 0.69 µg mL -1 to 2.44 µg mL -1 (LOD 0.21 µg mL -1 ) in the case of optimized SWAdSV. The relative standard deviation is below 1.5%. The reliability of the elaborated procedures and thus the accuracy of the obtained results were validated by comparing them with those obtained by means of HPLC-DAD measurements. The direct cathodic SWV method was successfully applied for the determination of EES in the pharmaceutical preparation Eritromicin ® , while SW-AdSV was applied in the case of the spiked urine sample.In both cases, the standard addition meth...

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Section: Introductionmentioning

confidence: 99%

Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation

Vajdle

Guzsvány

Škorić

et al. 2016

Electrochimica Acta

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“…Estes resíduos podem causar efeitos tóxicos aos consumidores, como reações alérgicas em indivíduos sensíveis, provocar o desenvolvimento de bactérias antibiótico-resistentes e induzir a resistência cruzada entre outros antibióticos de estrutura ou mecanismo de ação similar, tornando mais difícil o tratamento de determinadas infecções. Contudo, a resistência bacteriana é a principal preocupação em relação ao uso da tilosina e demais macrolídeos [28,29] .…”

Section: Figura 1: Estrutura Química Da Tilosina Aunclassified

“…Estes métodos são excelentes como meios semiquantitativos de triagem de quantidades residuais de macrolídeos em amostras de leite, porém não são suficientemente específicos para assegurar a identificação precisa das drogas residuais nos tecidos animais. Para superar estes problemas, as análises cromatográficas, tais como a Cromatografia Líquida de Alta Eficiência com detector de Ultravioleta (CLAE-UV), com detecção electroquímica, e mais recentemente a Espectrometria de Massas (EM), considerada o meio de detecção mais universal, além da eletroforese capilar têm sido relatadas para a determinação de macrolídeos [47,29] .…”

Section: Microbiológicas Cromatográficas Imunoensaiosunclassified

Tilosina: um importante antibiótico não monitorado em leite no Brasil

Costa

Spisso

Pereira

et al. 2015

Segur. Aliment. Nutr.

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A introdução de agentes antimicrobianos na alimentação animal revolucionou a produção de alimentos no mundo, porém o uso indiscriminado desses agentes podem provocar agravos à saúde da população. Os macrolídeos constituem a segunda classe de antimicrobianos mais usados nos animais em geral, sendo a tilosina presente em um maior número de formulações disponíveis para bovinos. A contaminação do leite por resíduos de tilosina acima dos valores considerados seguros pode constituir um risco potencial à saúde do consumidor. Com o objetivo de obter uma visão geral da atual situação da segurança de alimentos em relação a este problema, este estudo foi realizado mediante levantamento bibliográfico na base de dados SciFinder Scholar e periódicos CAPES. Foi constatado que no Brasil existem dois programas em andamento para avaliar a presença de resíduos de medicamentos veterinários em alimentos: PNCRC e PAMVet. Entretanto, a tilosina não consta no escopo analítico destes programas. Dos artigos que tratam da contaminação do leite por resíduos de tilosina publicados entre 2007 e 2013, oito abordam o monitoramento desta droga e vinte e dois o desenvolvimento/teste de performance de novas técnicas. Estes resultados alertam para que maior número de estudos de monitoramento da tilosina no leite seja realizado a fim de se estabelecer políticas de segurança alimentar e controle dessa substância, além de investigar se há uso indiscriminado desta droga.Palavras-chave: leite, monitoramento, tilosina. Tylosin: an important antibiotic not monitored in milk in BrazilThe use of antibiotics in animal feed has brought changes to food production worldwide, but the indiscriminate use of these drugs can be harmful to human beings. In general, macrolides are the second class of antibiotics most used in animals, in which tylosin is found in a large number of formulations available for livestock. Contaminated milk by residues of tylosin above safe values can become a potential risk to the health of consumers. Due to the purpose of having an overview of the current food safety situation regarding this problem, this study was done based on a bibliographical survey at database SciFinder Scholar and periodicals from CAPES. It was found that in Brazil, there are two current programs

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“…The presence of antibiotics like tylosin in milk can be very problematic because their residues can have unpleasant effects on consumer health (development of allergic reactions, appearance of resistant bacteria, etc. ), and they are suspected of causing cross‐resistance to other antibiotics of similar structure or mechanism of action, making it more difficult to treat certain infections . The EU and the USA Food and Drug Administration (FDA) have found it crucial for macrolides in food products .…”

Section: Introductionmentioning

confidence: 99%

Extraction and preconcentration of tylosin from milk samples through functionalized TiO₂nanoparticles reinforced with a hollow fiber membrane as a novel solid/liquid-phase microextraction technique

et al. 2014

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The aim of this study was to introduce a novel, simple, and highly sensitive preparation method for determination of tylosin in different milk samples. In the so-called functionalized TiO2 hollow fiber solid/liquid-phase microextraction method, the acceptor phase is functionalized TiO2 nanoparticles that are dispersed in the organic solvent and held in the pores and lumen of a porous polypropylene hollow fiber membrane. An effective functionalization of TiO2 nanoparticles has been done in the presence of aqueous H2 O2 and a mild acidic ambient under UV irradiation. This novel extraction method showed excellent extraction efficiency and a high enrichment factor (540.2) in comparison with conventional hollow fiber liquid-phase microextraction. All the experiments were monitored at λmax = 284 nm using a simple double beam UV-visible spectrophotometer. A Taguchi orthogonal array experimental design with an OA16 (4(5) ) matrix was employed to optimize the factors affecting the efficiency of hollow fiber solid/liquid-phase microextraction such as pH, stirring rate, salt addition, extraction time, and the volume of donor phase. This developed method was successfully applied for the separation and determination of tylosin in milk samples with a linear concentration range of 0.51-7000 μg/L (r(2) = 0.991) and 0.21 μg/L as the limit of detection.

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Matrix solid-phase dispersion method for the determination of macrolide antibiotics in sheep’s milk

Cited by 48 publications

References 22 publications

Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation

Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation

Tilosina: um importante antibiótico não monitorado em leite no Brasil

Extraction and preconcentration of tylosin from milk samples through functionalized TiO₂nanoparticles reinforced with a hollow fiber membrane as a novel solid/liquid-phase microextraction technique

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Matrix solid-phase dispersion method for the determination of macrolide antibiotics in sheep’s milk

Cited by 48 publications

References 22 publications

Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation

Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation

Tilosina: um importante antibiótico não monitorado em leite no Brasil

Extraction and preconcentration of tylosin from milk samples through functionalized TiO2nanoparticles reinforced with a hollow fiber membrane as a novel solid/liquid-phase microextraction technique

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Product

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About

Extraction and preconcentration of tylosin from milk samples through functionalized TiO₂nanoparticles reinforced with a hollow fiber membrane as a novel solid/liquid-phase microextraction technique