Matrix molecules and growth factors as indicators of periodontal disease activity

Giannobile, William V.; Al-Shammari, Khalaf F.; Sarment, David P.

doi:10.1034/j.1600-0757.2003.03108.x

Cited by 87 publications

(120 citation statements)

References 105 publications

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“…GAGs, the long linear polysaccharides consisting of a repeating disaccharide unit, are major noncollagenous components of extracellular matrix. This group of macromolecules has received the attention of dental researchers as a potential indicator for periodontal diseases (Last et al, 1985;Smith et al, 1995;Giannobile et al, 2003). The abundance of all three genes involved in GAG degradation was dramatically elevated in periodontitis as demonstrated previously.…”

Section: Discussionmentioning

confidence: 84%

“…Of particular interest to us is the gene encoding N-acetyl-D-galactosamine-4-sulfate 4-sulfohydrolase associated with C-4-S degradation because the gingival crevicular fluid content of C-4-S exhibited significant correlations with probing depth and attachment loss (Last et al, 1985;Smith et al, 1995) and C-4-S is regarded as a biomarker for bone degradation as it accounts for 94% of GAGs in alveolar bone (Waddington et al, 1989;Armitage, 2004). The higher concentrations of C-4-S and other GAGs in periodontitis (Last et al, 1985;Smith et al, 1995;Giannobile et al, 2003) seem to contradict the observation that degradation genes were enriched. Reasons might be because, first, the functional gene composition is a reflection of a shift in the community composition and does not necessarily represent the overexpression of GAG degradation enzymes.…”

Section: Discussionmentioning

confidence: 99%

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Phylogenetic and functional gene structure shifts of the oral microbiomes in periodontitis patients

et al. 2014

The ISME Journal

146

126

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Determining the composition and function of subgingival dental plaque is crucial to understanding human periodontal health and disease, but it is challenging because of the complexity of the interactions between human microbiomes and human body. Here, we examined the phylogenetic and functional gene differences between periodontal and healthy individuals using MiSeq sequencing of 16S rRNA gene amplicons and a specific functional gene array (a combination of GeoChip 4.0 for biogeochemical processes and HuMiChip 1.0 for human microbiomes). Our analyses indicated that the phylogenetic and functional gene structure of the oral microbiomes were distinctly different between periodontal and healthy groups. Also, 16S rRNA gene sequencing analysis indicated that 39 genera were significantly different between healthy and periodontitis groups, and Fusobacterium, Porphyromonas, Treponema, Filifactor, Eubacterium, Tannerella, Hallella, Parvimonas, Peptostreptococcus and Catonella showed higher relative abundances in the periodontitis group. In addition, functional gene array data showed that a lower gene number but higher signal intensity of major genes existed in periodontitis, and a variety of genes involved in virulence factors, amino acid metabolism and glycosaminoglycan and pyrimidine degradation were enriched in periodontitis, suggesting their potential importance in periodontal pathogenesis. However, the genes involved in amino acid synthesis and pyrimidine synthesis exhibited a significantly lower relative abundance compared with healthy group. Overall, this study provides new insights into our understanding of phylogenetic and functional gene structure of subgingival microbial communities of periodontal patients and their importance in pathogenesis of periodontitis.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Phylogenetic and functional gene structure shifts of the oral microbiomes in periodontitis patients

et al. 2014

The ISME Journal

146

126

View full text Add to dashboard Cite

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“…25 Para além do seu papel na regulação da angiogénese também se pensa que o VEGF desempenhe um papel importante na regulação da remodelação óssea, nomeadamente, na atração de células endoteliais e osteoclastos e estimulando a diferenciação dos osteoblastos. 25 A sua possível contribuição para a progressão da doença periodontal foi suportada por um estudo que reporta a presença de concentrações mais elevadas de VEGF em gengivas inflamadas adjacentes a bolsas com profundidades de sondagem de 4 -6 mm.…”

Section: Amp Studentunclassified

Tireoidite de Hashimoto e Doença Periodontal: Uma Revisão Narrativa

2016

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RESUMOIntrodução: Atualmente há um crescente interesse no estudo das condições sistémicas com repercussões no periodonto. O objetivo deste artigo é determinar se existe uma relação entre a tireoidite de Hashimoto e a doença periodontal. Material e Métodos:Fundamentada na periodontologia baseada na evidência e na combinação das expressões: 'Hashimoto disease'; 'Hypothyroidism'; 'Periodontal diseases'; 'Systemic diseases'; optou-se pela pesquisa e avaliação de artigos presentes nas bases de dados Medline, Scopus e Thomson Reuters, selecionando 30 para a análise na íntegra.Resultados: Vários são os trabalhos desenvolvidos na procura de um melhor entendimento sobre a complexidade e patogénese das doenças periodontais, associando-as a múltiplas condições sistémicas. Atualmente a relação melhor descrita na literatura é a da artrite reumatoide; contudo, outras relações têm sido apontadas como é o caso da tireoidite de Hashimoto.Discussão: A identificação de múltiplos mecanismos etiopatogénicos comuns à tireoidite de Hashimoto e à doença periodontal permite suspeitar de uma relação entre ambas. São exemplos a proliferação de linfócitos T helper 1 e 17 e suas repercussões periodontais, a disfunção do endotélio vascular da microcirculação gengival e a influência do hipotiroidismo sobre o metabolismo ósseo, nomeadamente ao nível do osso alveolar. Conclusão:Existe plausibilidade biológica que suporta o estabelecimento de uma relação de associação entre a tireoidite de Hashimoto e a doença periodontal. Contudo, os estudos atuais não são suficientes para comprovarem um nexo causal entre estas duas patologias, devendo, os mesmos, ser elaborados num futuro próximo, de forma a melhor determinar a sua relação e interação.Palavras-chave: Doenças de Hashimoto; Doença Periodontal; Revisão. ABSTRACT Introduction:Currently there is a growing interest in studying systemic conditions with impact on the periodontium. The aim of this article is to determinate if there is a relation between Hashimoto's thyroiditis and periodontal disease. Results: There have been developed several studies, searching for a better comprehension about the complexity and pathogenesis of periodontal diseases, associated them to multiple systemic conditions. Actually, the relationship that is best described in the literature is the one with rheumatoid arthritis; however, other relations have been pointed, such as Hashimoto's thyroiditis. Discussion:The identification of multiple etiopathogenic mechanisms common to Hashimoto's thyroiditis and periodontal disease allow to suspect of a relation between them. Some of these mechanisms include the proliferation of lymphocytes T helper 1 and T helper 17 and their impact on the periodontium, the dysfunction of vascular endothelium in gingival microcirculation and the influence of hypothyroidism on bone metabolism, namely on the alveolar bone. Conclusion:There is biological plausibility to support the establishment of an association between Hashimoto's thyroiditis and periodontal disease. However, there are not enough st...

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“…Other authors reported increased VEGF expression in epithelial cells and endothelial cells in periodontitis-affected gingiva (Guneri et al, 2004;Keles et al, 2010;Lucarini et al, 2009). Giannobile et al (2003) suggested that VEGF could be an important growth factor for the onset of gingivitis and its progression to periodontitis. Taken together these observations conclude that VEGF expression is related to both maintenance of periodontal health and periodontal tissue destruction but the precise mechanism of neovascularization remains in debate.…”

Section: G) Vascular Endothelial Growth Factor (Vegf)mentioning

confidence: 99%

“…Advances in periodontal diseases diagnostic research are moving toward methods whereby periodontal risk can be identified and quantified by objective measures such as biomarkers. Gingival crevicular fluid (GCF) and salivary levels of several growth factors, cytokines and enzymes of host origin appear to hold the greatest promise as valuable biomarkers in assessing development of periodontal disease (Buduneli & Kinane, 2011;Giannobile et al, 2003;Gurkan et al, 2008;Goncalves et al, 2009;Kaufman & Lamster, 2000;Pisoschi et al, 2010;Wright et al, 2000).…”

Section: Introductionmentioning

confidence: 99%