Mating Disruption of Grapholita molesta by RNA Interference of a Fatty Acid Desaturase Expressed in Adult Abdomen

In moths, various enzymes, such as fatty acid synthases, fatty acyl desaturases, and fatty acyl reductases (FARs), are involved in pheromone biosynthesis. In particular, pheromone gland‐specific FAR (pgFAR) plays an important role in converting the functional group from carboxylic to alcohol during pheromone biosynthesis. A novel pgFAR of Maruca vitrata, Mvi‐pgFAR, was identified through transcriptome sequencing of its pheromone gland. To investigate the involvement of Mvi‐pgFAR in pheromone biosynthesis, Mvi‐pgFAR was cloned from the pheromone gland and suppressed by RNA interference (RNAi). Mvi‐pgFAR harbored several conserved motifs related to NAD(P)H‐binding, N‐glycosylation, and adenosine / guanosine triphosphate binding. Phylogenetic analysis revealed that Mvi‐pgFAR with other lepidopteran pgFARs formed an independent clade. Mvi‐pgFAR was specifically expressed only in the pheromone gland. Quantitative real‐time polymerase chain reaction showed that the diurnal expression levels of Mvi‐pgFAR in the pheromone gland were the highest at 2 h before the scotophase. After primarily confirming Mvi‐pgFAR suppression by RNAi, (E,E)‐10,12‐hexadecadienal (E10E12‐16:Ald), a major sex pheromone component, was quantified by gas chromatography. When Mvi‐pgFAR was successfully suppressed, E10E12‐16:Ald production was reduced by up to half of that of the control, and the mating rate was subsequently decreased. Our results demonstrate that Mvi‐pgFAR downregulation can suppress mating behavior by changing the relative sex pheromone component ratio, suggesting that Mvi‐pgFAR can be used as a novel control target.

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“…Grapholita molesta reduced the attractiveness of virgin females in apple orchards (Kim et al, 2017).…”

Section: Discussionmentioning

confidence: 95%

“…In Plutella xylostella , suppression of the PBAN receptor in female adults resulted in a 20%–40% reduction in mating rate (Lee et al ., 2011). Suppression of fatty acyl desaturase, Δ10 desaturase from Grapholita molesta reduced the attractiveness of virgin females in apple orchards (Kim et al ., 2017).…”

Section: Discussionmentioning

confidence: 99%