Mathematical modeling of Kluyveromyces marxianus growth in solid-state fermentation using a packed-bed bioreactor

Abstract

This work investigated the growth of Kluyveromyces marxianus NRRL Y-7571 in solid-state fermentation in a medium composed of sugarcane bagasse, molasses, corn steep liquor and soybean meal within a packed-bed bioreactor. Seven experimental runs were carried out to evaluate the effects of flow rate and inlet air temperature on the following microbial rates: cell mass production, total reducing sugar and oxygen consumption, carbon dioxide and ethanol production, metabolic heat and water generation. A mathematical model based on an artificial neural network was developed to predict the above-mentioned microbial rates as a function of the fermentation time, initial total reducing sugar concentration, inlet and outlet air temperatures. The results showed that the microbial rates were temperature dependent for the range 27-50 degrees C. The proposed model efficiently predicted the microbial rates, indicating that the neural network approach could be used to simulate the microbial growth in SSF.

Efficient growth of Kluyveromyces marxianus biomass used as a biocatalyst in the sustainable production of ethyl acetate

“…Stoichiometric equations for describing the growth of K. marxianus has been derived here by using the method of Hensing et al [39] and Mazutti et al [69]. Such balancing requires a sum formula for biomass.…”

Production of protein and metabolites by yeast grown in solid state fermentation: present status and perspectives

“…These kinds of processes have been adapted to bio‐ethanol production from wet sorghum stalks . However, there is recent work on the use of yeasts for the conversion of xylose to xylitol by Candida guillermondi grown on sugar cane bagasse and lipase production from Yarrowia lipolytica grown on olive waste, pullulan from Aureobasidium pullulans grown on cassava residues or biomass of Kluyveromyces marxianus grown sugar cane bagasse fortified with molasses, corn steep liquor and soy bean meal . Those types of products require little recovery expense because the final products are used as crude preparations.…”

Production of bioherbicide by Phoma sp. in a stirred-tank bioreactor

“…to produce a bioherbicide by solid-state fermentation (Klaic et al 2016), with promising results. However, the scale-up of solid-state fermentation process is complicated due to difficult to remove the metabolic heat generated during the process (Mazutti et al 2010). One possibility is the production of bioherbicide by submerged fermentation, which is a process well established industrially.…”