Production of protein and metabolites by yeast grown in solid state fermentation: present status and perspectives

Abstract

Culture conditions for the generation of products using yeast have been optimized for fermentative processes in industry involving predominantly submerged medium (SmF). However, solid‐state fermentation (SSF) is now a realistic alternative system for the production of recombinant proteins and metabolites of interest in the market, with great potential in biofuels production, food, chemical and pharmaceutical industries. One of the main advantages of SSF over SmF is the reduction of downstream expenses. Also, the use of artificial and very cheap solid supports for yeast SSF such as polyurethane foam or amberlite helps with study of the physiology of such systems. This mini‐review makes an overview of previous research and emphasizes the major physiological advantages of yeast SSF that can be used for new processes and product development and stresses the need for integrated approaches between adaptive evolution and high‐throughput genetic analysis. © 2015 Society of Chemical Industry

Differences in growth physiology and aggregation of Pichia pastoris cells between solid‐state and submerged fermentations under aerobic conditions

“…). Differences concerning the structure of the cell aggregates in the culture observed through microscopy are more likely related to oxygen transfer and osmotic stress; both parameters are consistent with evolution of yeasts in a natural system more similar to the SSF environment . Until now, most of the reports on yeast biofilms have been analyzed either for Candida albicans (with clinical intention) or Saccharomyces cerevisiae ; the analysis presented here compares for the first time the structure of P. pastoris aggregates in SSF and in SmF for industrial purposes.…”

“…Differences concerning the structure of the cell aggregates in the culture observed through microscopy are more likely related to oxygen transfer and osmotic stress; both parameters are consistent with evolution of yeasts in a natural system more similar to the SSF environment. 16 Until now, most of the reports on yeast biofilms have been analyzed either for Candida albicans (with clinical intention) or Saccharomyces cerevisiae; 17,18 the analysis presented here compares for the first time the structure of P. pastoris aggregates in SSF and in SmF for industrial purposes. Three interesting perspectives can be seen from these observations; the first is the development of new materials with potential use as supports in SSF cultivation, focused on maximizing the area/volume ratio to optimize the yield in terms of biomass production.…”

Solid-state fermented plant protein sources in the diets of broiler chickens: A review

“…Various kinds of microorganisms are used in SSF. Due to the minimal moisture required, only a limited number of microorganisms such as yeast and filamentous fungi can grow well under SSF conditions (López-Pérez and Viniegra-González, 2016). Although, there are indications of the successful use of some bacteria strains in producing bioproducts from SSF (Couto and Sanromán, 2006, Singhania et al., 2009, Martins et al., 2011).…”

Solid-State Fermentation as a Novel Paradigm for Organic Waste Valorization: A Review

“…However, fungi and yeast are the most commonly reported for the reason that SSF can provide a similar natural habitat, which has low water activity in the fermentation media. The most common yeast genera reported in SSF are Candida, Saccharomyces and Aureobasidium [71], while common fungal genera are Aspergillus, Penicillium, and Rhizopus, which have a broad range of species for the production of a wide number of valuable bio-products through SSF [12]. Despite SSF seeming to be more favorable for fungi and yeast growth, there is also increasing evidence of bacteria successfully used in producing bio-products in SSF.…”

“…As a matter of fact, the process requires low consumption of energy since agitation and sterilization are not always necessary. Similarly, this process reduces purification costs due to higher end product concentration or by using the product in a crude form [71]. …”