Materials and techniques for electrochemical biosensor design and construction

Zhang, S; Wright, G.; Yang, Ying

doi:10.1016/s0956-5663(00)00076-2

Cited by 232 publications

(86 citation statements)

References 78 publications

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“…In the case of phenols, fungal laccase has generally optimal activity in the pH range between 3.0 and 7.0 (BOLLAG; LEONOWICZ, 1984). An optimal pH response for biosensors using immobilized laccase in other systems has been reported between 4.0 for laccase immobilized in chitosan microspheres (FERNANDES et al, 2008) Several recent studies on enzyme immobilization have focused on different materials and methods for attaching the biologic agent to an insoluble support, basically as biosensors (ZHANG et al, 2000;FERNÁNDEZ-FERNÁNDEZ et al, 2012). Carbon-based materials have been successfully used in the construction of these devices (JAROSZ-WILKOLAZKA et al, 2004;LIU et al, 2006;SANTHIAGO;VIEIRA, 2007;FERNANDES et al, 2008;OLIVEIRA et al, 2013).…”

Section: Effect Of Laccase (Aspergillus Oryzae; Novozym 51003mentioning

confidence: 99%

<b>Effect of mediator added to modified paste carbon electrodes with immobilized laccase from <i>Aspergillus oryzae

et al. 2015

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ABSTRACT. Carbon paste electrodes based on the immobilization of laccase from Aspergillus oryzae were developed and voltammetric measurements were performed to evaluate the amperometric response. The 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) functions as substrate and mediator for the laccase enzyme. Electrodes were modified in two different conditions: without mediator (EPC/laccase) and with mediator (EPC/laccase/ABTS). The addition of ABTS as a mediator increased eight-fold the amperometric response. The electrode was sensitive to pH variation with best response at pH 4.0. Studies on different concentrations of laccase and ABTS at different pH rates revealed that the composition 187 U mL -1 in laccase and 200 μL of ABTS obtained the highest amperometric response. The carbon paste electrode modified with ABTS proved to be a good base for the immobilization of the laccase enzyme. Moreover, it is easy to manufacture and inexpensive to produce a modified electrode with potential application in biosensors.Keywords: cyclic voltammetry, immobilization, ABTS, biosensors. Efeito da adição de mediador em eletrodos de pasta de carbono contendo lacase de Aspergillus oryzae RESUMO. Foram desenvolvidos eletrodos de pasta de carbono baseados na imobilização de lacase deAspergillus oryzae e foram realizadas medidas voltamétricas para verificar a resposta amperométrica. O sal de 2,2′-azino-bis 3-etilbenzotiazolina-6-sulfonato (ABTS) funciona como mediador e substrato para a enzima lacase. Foram modificados eletrodos em duas condições especificas: sem o mediador (EPC/lacase) e com o mediador (EPC/lacase/ABTS). A adição do ABTS como mediador aumentou a resposta amperométrica em oito vezes. Este eletrodo se mostrou sensível à variação de pH obtendo melhor resposta em pH 4,0. Estudos que envolvem diferentes concentrações de lacase e ABTS, em diferentes valores de pH revelaram que a composição 187 U mL -1 de lacase e 200 μL de ABTS obteve a maior resposta amperométrica. A pasta de carbono se mostrou um bom suporte para imobilização da enzima lacase, além de ser de fácil fabricação e de baixo custo para possível aplicação como biossensor.Palavras-chave: voltametria cíclica, imobilização, ABTS, bissensores.

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Section: Effect Of Laccase (Aspergillus Oryzae; Novozym 51003mentioning

confidence: 99%

<b>Effect of mediator added to modified paste carbon electrodes with immobilized laccase from <i>Aspergillus oryzae

et al. 2015

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“…The incorporation of biologically active molecules into sensing devices has promoted considerable development of fast, efficient and inexpensive assays for a wide range of analytes in research, clinical, industrial and environmental applications (Braguglia, 1998;Zhang et al, 2000;Karube and Nomura, 2000;Liang et al, 2000;Wang, 2001). In particular, the immobilization of enzymes onto electrode surfaces provides biosensors that can be categorized broadly as first, second or third generation devices depending on the mode of electron transfer between the redox center of the enzyme and the electrode surface (Wang, 1993;Scheller et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Characterization in vitro and in vivo of the oxygen dependence of an enzyme/polymer biosensor for monitoring brain glucose

Dixon

Lowry

O’Neill

2002

Journal of Neuroscience Methods

108

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The oxygen dependence of a first generation amperometric biosensor was investigated in vitro and in vivo by monitoring its glucose response as a function of solution pO 2 . The biosensor was a glucose oxidase (GOx) modified poly(o -phenylenediamine) coated Pt cylinder electrode (Pt/PPD/GOx) that has been designed for neurochemical analysis in vivo. Two types of oxygen probes were used: a self-calibrating commercial macroelectrode in vitro; and a carbon paste microelectrode in vivo. Calibrations in vitro showed that oxygen interference in the operation of Pt/PPD/GOx electrodes was minimal for concentrations of glucose ( Â/0.5 mM) and oxygen ( Â/50 mM) found in brain ECF. This observation was confirmed by simultaneous monitoring in vivo of brain glucose and oxygen in the awake rat. However, at levels of glucose normally found in peripheral tissues ( Â/5 mM), the oxygen dependence was severe. We conclude that the oxygen sensitivity of Pt/PPD/GOx biosensors does not preclude their reliable use in media containing low glucose levels, such as brain ECF. #

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“…thickness and stability of the materials), the immobilization method and the detection technique are relevant factors that must be considered whenever developing an electrochemical aptasensor (Zhang et al, 2000). Usually, a three-electrode configuration is included in the surface of a voltammetric aptasensor, namely the working, auxiliary or counter and the reference electrodes (Lim et al, 2010).…”

Section: Electrode Surfacementioning

confidence: 99%

“…The common material used as the working electrode surface is carbon and noble metals, for which different immobilization strategies and detection approaches can be applied. Metals such as gold, platinum, silver and stainless steel have long been used for electrochemical electrodes due to their excellent electrical and mechanical properties (Hayat and Marty, 2014;Ronkainen et al, 2010;Zhang et al, 2000). Other electrode surfaces can be used in the development of electrochemical aptasensors, such as screen printed electrodes (SPE), glassy carbon electrodes (GCE), carbon paste electrodes (CPE), depending on the target molecule to be detected.…”

Section: Electrode Surfacementioning

confidence: 99%

Voltammetric aptasensors for protein disease biomarkers detection: A review

Meirinho

Dias

Peres

et al. 2016

Biotechnology Advances

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An electrochemical aptasensor is a compact analytical device where the bioreceptor (aptamer) is coupled to a transducer surface to convert a biological interaction into a measurable signal (current) that can be easily processed, recorded and displayed. Since the discovery of the Systematic Evolution of Ligands by Enrichment (SELEX) methodology, the selection of aptamers and their application as bioreceptors has become a promising tool in the design of electrochemical aptasensors. Aptamers present several advantages that highlight their usefulness as bioreceptors such as chemical stability, cost effectiveness and ease of modification towards detection and immobilization at different transducer surfaces. In this review, a special emphasis is given to the potential use of electrochemical aptasensors for the detection of protein disease biomarkers using voltammetry techniques. Methods for the immobilization of aptamers onto electrode surfaces are discussed, as well as different electrochemical strategies that can be used for the design of aptasensors.

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Materials and techniques for electrochemical biosensor design and construction

Cited by 232 publications

References 78 publications

<b>Effect of mediator added to modified paste carbon electrodes with immobilized laccase from <i>Aspergillus oryzae

<b>Effect of mediator added to modified paste carbon electrodes with immobilized laccase from <i>Aspergillus oryzae

Characterization in vitro and in vivo of the oxygen dependence of an enzyme/polymer biosensor for monitoring brain glucose

Voltammetric aptasensors for protein disease biomarkers detection: A review

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