&lt;b&gt;Effect of mediator added to modified paste carbon electrodes with immobilized laccase from &lt;i&gt;Aspergillus oryzae

Ferreira, Marcelo Silva Ângelo; Filho, Arnaldo Fernandes da Silva; Martins, Vitor L.; Andrade, Milena Martins; Giarola, Danilo Antonio; Dall’Antônia, Luiz Henrique

doi:10.4025/actascitechnol.v37i2.19660

Cited by 2 publications

(1 citation statement)

References 28 publications

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“…According to the type of substrate, the optimal pH is different [42]; fungal Lac shows optimal activity in the pH range from 3 to 7 for the phenolic compound [43]. The optimum pH was found to be 3 and it agrees with previous experiments [44] and native Lac activity [45]. As per pre-reported data, the activity of the Lac enzyme occurs in acidic media.…”

Section: Effect Of Ph: the Proposed Biosensor Was Embraced In Buffer supporting

confidence: 86%

Novel fabrication of a laccase biosensor to detect phenolic compounds using a carboxylated multiwalled carbon nanotube on the electropolymerized support

et al. 2019

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Biosensors research is one of the fastest growing fields in which tens of thousands of papers have been published over the years; even more, numerous biosensors have been developed for the detection of phenolic compounds, such as catechol which reacts with an appropriate enzymatic bioreceptor like laccase. A biosensing electrode for catechol detection was investigated by covalent immobilization of laccase on a glassy carbon electrode modified by conducting polymers built of poly(3,4-ethylenedioxythiophene), gold nanoparticles and carboxylated multiwalled carbon nanotubes. The fabrication process of the sensing surface was investigated by Fourier transform infrared spectroscopy, scanning electron microscopy and electrochemical procedures. The electrochemical results demonstrate that the enzyme was immobilized covalently onto the modified glassy carbon electrode by the interaction between carboxyl groups of the carboxylated multiwalled carbon nanotubes and laccase. The biosensor demonstrates a direct electron transfer between the electrode and immobilized laccase. Under optimum conditions, it presented two linear responses in the range of 0.1-0.5 and 11.99-94.11 μM. The limits of detection were found to be 0.11 and 12.26 μM.

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Section: Effect Of Ph: the Proposed Biosensor Was Embraced In Buffer supporting

confidence: 86%

Novel fabrication of a laccase biosensor to detect phenolic compounds using a carboxylated multiwalled carbon nanotube on the electropolymerized support

et al. 2019

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Effects of wilting and additives on the ensiling quality and in vitro rumen fermentation characteristics of sudangrass silage

et al. 2021

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Objective: To investigate the effects of molasses and Lactobacillus plantarum on the ensiling quality and in vitro rumen fermentation of sudangrass silage prepared with or without wilting. Methods: The ensiling experiment, measured with 3 replicates, was carried out according to a 2 × 4 (wilted stages × additives) factorial treatment structure. Dry matter of the fresh (210 g/kg fresh matter) or wilted (305 g/kg fresh matter) sudangrass were ensiled (packed into 5.0-L plastic jars) without additive (control) or with molasses (M), Lactobacillus plantarum (LP), or molasses + Lactobacillus plantarum (M+LP). After 60 days of ensiling, the silages were analyzed for the chemical, fermentation and in vitro characteristics. Results: After 60 days of ensiling, the fermentation parameters were affected by wilted, the additives and the interactions of wilted with the additives (p<0.05). The M+LP treatment at wilted had higher lactic acid levels and V-score (p<0.05) but lower pH values and butyric acid concentrations than the other treatments. In comparison with sudangrass before ensiling, after ensiling had lower dry matter and higher non-fibrous carbohydrate. The in vitro gas production, in vitro dry matter digestibility, in vitro crude protein digestibility, and in vitro acid fiber detergent digestibility changed under the effects of the additives. Significant interactions were observed between wilted and the additives in terms of in vitro gas production at 48 h, asymptotic gas production, gas production rate, half time, and the average gas production rate. The total volatile fatty acid levels in the additive treatments were higher than those in the control. Conclusion: Wilting and supplementation with molasses and Lactobacillus plantarum had the ability to improve the ensiling quality and in vitro nutrient digestibility of sudangrass silage. The M+LP treatment at wilted exhibited the strongest positive effects on silage quality and in vitro ruminal fermentation characteristics.

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<b>Effect of mediator added to modified paste carbon electrodes with immobilized laccase from <i>Aspergillus oryzae

Cited by 2 publications

References 28 publications

Novel fabrication of a laccase biosensor to detect phenolic compounds using a carboxylated multiwalled carbon nanotube on the electropolymerized support

Novel fabrication of a laccase biosensor to detect phenolic compounds using a carboxylated multiwalled carbon nanotube on the electropolymerized support

Effects of wilting and additives on the ensiling quality and in vitro rumen fermentation characteristics of sudangrass silage

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