Massilia arvi sp. nov., isolated from fallow-land soil previously cultivated with Brassica oleracea, and emended description of the genus Massilia

Abstract

Massilia arvi sp. nov., isolated from fallow-land soil previously cultivated with Brassica oleracea, and emended description of the genus Massilia A novel bacterial strain, designated THG-RS2O T , was isolated from fallow-land soil previously cultivated with Brassica oleracea in Yongin, South Korea. Cells were Gram-stain-negative, aerobic, non-motile rods, catalase-and oxidase-positive. Strain THG-RS2O T grew optimally at 25-37 8C, at pH 7.0 and in the absence of NaCl. 16S rRNA gene sequence analysis demonstrated that strain THG-RS2O T shows highest sequence similarity with Massilia kyonggiensis KACC 17471 T followed by Massilia aerilata KACC 12505 T , Massilia niastensis KACC 12599 T , Massilia tieshanensis KACC 14940 T and Massilia haematophila KCTC 32001 T . Levels of DNA-DNA relatedness between strain THG-RS2O T and the closest phylogenetic neighbours were below 55.0 % and the DNA G+C content of strain THG-RS2O T was 63.2 mol%. Major fatty acids were C 16 : 0 , cyclo-C 17 : 0 and summed feature 3 (C 16 : 1 v6c and/or C 16 : 1 v7c). The major respiratory quinone was identified as ubiquonone-8 and predominant polar lipids were determined to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Characterization by 16S rRNA gene sequence analysis, DNA-DNA hybridization, ubiquinone, polar lipid, fatty acid composition, and physiological and biochemical parameters revealed that strain THG-RS2O T represents a novel species of the genus Massilia. Hence, the present study describes a novel species for which the name Massilia arvi sp. nov. is proposed. The type strain is THG-RS2O T (5KCTC 42609 T 5CCTCC AB 2015115 T ).

Massilia arenae sp. nov., isolated from sand soil in the Qinghai–Tibetan Plateau

“…The major fatty acids in GEM5 T were summed feature 3 (46.2 %) and C 16 : 0 (25.5 %), which were consistent with those of Massilia timonae CCUG 45783 T and Massilia aurea AP13 T (Table S1). These results conformed to the characteristics of members of the genus Massilia [2, 40, 41].…”

Duganella lactea sp. nov., Duganella guangzhouensis sp. nov., Duganella flavida sp. nov. and Massilia rivuli sp. nov., isolated from a subtropical stream in PR China and proposal to reclassify Duganella ginsengisoli as Massilia ginsengisoli comb. nov.

“…in 2011 [8] and Singh et al . in 2015 [9], respectively. At the time of writing, over 40 novel species within this genus were validly published.…”

Massilia rhizosphaerae sp. nov., a rice-associated rhizobacterium with antibacterial activity against Ralstonia solanacearum

“…solanacearum was poured into each pot. During the experiment [1], only the strain NEAU-GH312 T suspension was poured into the pots, ‘NEAU-GH312 T ’ [2]; no microbial suspension was added to the soil, ‘Blank’ [3]; only the pathogen R. solanacearum suspension was poured into the pots, ‘ R.…”

“…ml −1 ) of the pathogen R. solanacearum was poured into each pot. During the experiment [1], only the strain NEAU-GH312 T suspension was poured into the pots, 'NEAU-GH312 T ' [2]; no microbial suspension was added to the soil, 'Blank' [3]; only the pathogen R. solanacearum suspension was poured into the pots, 'R. solanacearum' [4]; both the NEAU-GH312 T suspension and R. solanacearum suspension were added to the soil, 'NEAU-GH312 T +R.…”