Mass Spectrometry‐Based Analysis of Time‐Resolved Proteome Quantification

Abstract

The aspect of time is essential in biological processes and thus it is important to be able to monitor signaling molecules through time. Proteins are key players in cellular signaling and they respond to many stimuli and change their expression in many time-dependent processes. Mass spectrometry (MS) is an important tool for studying proteins, including their posttranslational modifications and their interaction partners-both in qualitative and quantitative ways. In order to distinguish the different trends over time, proteins, modification sites, and interacting proteins must be compared between different time points, and therefore relative quantification is preferred. In this review, the progress and challenges for MS-based analysis of time-resolved proteome dynamics are discussed. Further, aspects on model systems, technologies, sampling frequencies, and presentation of the dynamic data are discussed.

A Comparative Proteomic Analysis to Explore the Influencing Factors on Endometritis Using LC-MS/MS

“…The dataset identifier IPX0004443000. https://www.iprox.cn/page/PSV023.html;?url=1677679444384dvFy (accessed on 5 March 2023) [3][4][5]. Passport: OkPc.…”

“…Uterine diseases, such as hysteritis and endometritis, are common in high-yield dairy goats and lead to economic losses due to a decline in milk production and fecundity [ 1 , 2 ]. Gram-negative bacteria are usually associated with uterine infection in dairy goats [ 2 , 3 ]. Dystocia and placental retention of dairy goats make them vulnerable to uterine diseases because the physical barrier of infection is broken and the immune response is disrupted, so pathogens should be eliminated [ 2 , 3 ].…”

“…Gram-negative bacteria are usually associated with uterine infection in dairy goats [ 2 , 3 ]. Dystocia and placental retention of dairy goats make them vulnerable to uterine diseases because the physical barrier of infection is broken and the immune response is disrupted, so pathogens should be eliminated [ 2 , 3 ].…”

“…Successful implantation requires a balance and accurate molecular communication between the embryo and the maternal endometrium [ 3 , 4 ]. Even a small imbalance could negatively affect the dialog between the mother and the embryo necessary for the establishment of pregnancy [ 4 ].…”

Comparative proteomic analysis of mild and severe endometritis determined by LC-MS/MS

“…One of the main factors in the pathogenicity of Escherichia coli is the interaction between lipopolysaccharide (LPS) and host tissue. The uterus is stimulated by E. coli lipopolysaccharide to cause in ammation of the endometrium 3 .…”