Mass spectrometric proteome analysis for profiling temperature‐dependent changes of protein expression in wild‐typeCaenorhabditis elegans

Abstract

We investigated the effect of cultivation temperatures on the protein expression levels in the fourth larval stage of the postembryonic development of wild-type Caenorhabditis elegans by mass spectrometric proteome analysis. From the 64 protein spots that were investigated, 5 spots were found reproducibly differently expressed when proteome maps derived from animals kept at 15 degrees C and at 25 degrees C, respectively, were compared. Spots of heat shock proteins HSP 70 (CE18679 or CE09682) and HSP 16 (CE14249) were present only in gels from protein extracts when worms were grown at 15 degrees C. Spots of two metabolic enzymes, the isocitrate dehydrogenase (CE10345) and the aspartic proteinase (CE21681) were detected only in cultures grown at the lower temperature as well. A protein with still unknown function (CE05036) was present only in gels from worm samples grown at 25 degrees C. We show for the first time by proteome analyses that cultivation of worms at the lowest temperature of the known physiological range (15 degrees C) already triggers a (weak) stress response in wild-type animals. This work led to the identification of "internal control proteins" in the wild-type strain for further characterization of temperature-sensitive strains using a proteomics approach.

Proteomic responses of blue mussel (Mytilus) congeners to temperature acclimation

“…For example, proteomic changes similar to those described here were shown in a study on the response to cold stress of livers of warm-adapted gilthead sea bream (Ibarz et al, 2010); a study on cultivation temperatures of wild-type C. elegans larvae also showed increasing levels of chaperones and IDH in response to cold (Madi et al, 2003).…”

Comparative genomics of nematodes

“…[68]). More recently temperature-dependent and stage-specific changes in the proteome [69] or germ-line developmentrelated proteins [70] have been determined. Protein interaction maps for C. elegans, derived from high-throughput yeast two-hybrid screens, are also being generated [71].…”

Evidence for only two independent pathways for decreasing senescence in Caenorhabditis elegans

“…Zebrafish, in response to cold temperature, increase specific antioxidant enzymes such as superoxide dismutase (SOD)-1 and SOD-2 (Malek et al 2004), and carp also show a specific gene expression profile in response to cold temperature that is reminiscent of those changes seen in calorically restricted mice (Gracey et al 2004;Han and Hickey 2005;Lee et al 1999Lee et al , 2000. C. elegans also displays a specific physiological change in response to cold temperatures (Madi et al 2003;Paul et al 2000), and it has even been noted that cold temperature is a type of mild stress, which suggests a possible hormetic effect. Wong et al have shown that clk-1 mutants are insensitive to temperature changes during embryogenesis, which demonstrates that developmental adjustments to changing temperatures is an active process requiring genes such as clk-1 (Wong et al 1995).…”