Marker vaccine potential of a foot-and-mouth disease virus with a partial VP1 G-H loop deletion

Fowler, Veronica L.; Knowles, Nick J.; Paton, David; Barnett, P.V.

doi:10.1016/j.vaccine.2010.02.074

Cited by 40 publications

(33 citation statements)

References 29 publications

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“…This indicated that the VP1 G-H loop may not be essential for the protection of natural hosts against FMDV. If this could be substantiated there would be potential to develop FMD marker vaccines, characterised by the absence of this region [198]. A mutant FMDV with deletion of immunodominant epitopes was evaluated to use as a marker vaccine recently, In that study, a B-cell epitope was identified in the 3A region of a nonstructural protein (NSP) by anti-FMDV cattle sera and a recombinant FMDV (rvAs-3A14D) was generated by selectively deleting 14 amino acids (position [91][92][93][94][95][96][97][98][99][100][101][102][103][104] in the 3A region of the NSP.…”

Section: Diva-based Companion Diagnosticmentioning

confidence: 99%

A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools

Longjam

Deb

Sarmah

et al. 2011

Veterinary Medicine International

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Foot-and-mouth disease (FMD) is one of the highly contagious diseases of domestic animals. Effective control of this disease needs sensitive, specific, and quick diagnostic tools at each tier of control strategy. In this paper we have outlined various diagnostic approaches from old to new generation in a nutshell. Presently FMD diagnosis is being carried out using techniques such as Virus Isolation (VI), Sandwich-ELISA (S-ELISA), Liquid-Phase Blocking ELISA (LPBE), Multiplex-PCR (m-PCR), and indirect ELISA (DIVA), and real time-PCR can be used for detection of antibody against nonstructural proteins. Nucleotide sequencing for serotyping, microarray as well as recombinant antigen-based detection, biosensor, phage display, and nucleic-acid-based diagnostic are on the way for rapid and specific detection of FMDV. Various pen side tests, namely, lateral flow, RT-LAMP, Immunostrip tests, and so forth. are also developed for detection of the virus in field condition.

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Section: Diva-based Companion Diagnosticmentioning

confidence: 99%

A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools

Longjam

Deb

Sarmah

et al. 2011

Veterinary Medicine International

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“…Complete replacement of the G-H loop was inefficient at protecting mice from homologous challenge, but partial G-H loop replacement appears to have prevented viremia and death in vaccinated animals (16). In a related experiment, Fowler et al (14) determined that partial loss of the FMDV serotype A G-H loop, immediately upstream of RGD and through the downstream alpha-helix (the structure of which was predicted to be lost by computational analysis), in inactivated vaccines did not generate broadened immunity or immune refocusing in cattle. The differences in results between these two studies were attributed to either different vaccination approaches (DNA vaccine versus inactivated virus) or the animal species tested (mice versus cattle).…”

Section: Discussionmentioning

confidence: 99%

Xenoepitope Substitution Avoids Deceptive Imprinting and Broadens the Immune Response to Foot-and-Mouth Disease Virus

Szczepanek

Barrette

Rood

et al. 2012

Clin. Vaccine Immunol.

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ABSTRACTMany RNA viruses encode error-prone polymerases which introduce mutations into B and T cell epitopes, providing a mechanism for immunological escape. When regions of hypervariability are found within immunodominant epitopes with no known function, they are referred to as “decoy epitopes,” which often deceptively imprint the host's immune response. In this work, a decoy epitope was identified in the foot-and-mouth disease virus (FMDV) serotype O VP1 G-H loop after multiple sequence alignment of 118 isolates. A series of chimeric cyclic peptides resembling the type O G-H loop were prepared, each bearing a defined “B cell xenoepitope” from another virus in place of the native decoy epitope. These sequences were derived from porcine respiratory and reproductive syndrome virus (PRRSV), from HIV, or from a presumptively tolerogenic sequence from murine albumin and were subsequently used as immunogens in BALB/c mice. Cross-reactive antibody responses against all peptides were compared to a wild-type peptide and ovalbumin (OVA). A broadened antibody response was generated in animals inoculated with the PRRSV chimeric peptide, in which virus binding of serum antibodies was also observed. A B cell epitope mapping experiment did not reveal recognition of any contiguous linear epitopes, raising the possibility that the refocused response was directed to a conformational epitope. Taken together, these results indicate that xenoepitope substitution is a novel method for immune refocusing against decoy epitopes of RNA viruses such as FMDV as part of the rational design of next-generation vaccines.

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“…Recently, an FMDV negative marker vaccine was developed by deleting partial VP1 G–H loop [43], and the subsequent result demonstrated that this FMD negative marker vaccine can fully protect cattle from experimental challenge and meet the DIVA purpose [35]. However, not only the G-H loop of the VP1 protein, but also other antigenic sites of the capsid proteins are responsible for the complete immunogenicity of the FMDV vaccine [44,45].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a 3A-truncated foot-and-mouth disease virus in pigs for its potential as a marker vaccine

Bai

et al. 2014

Vet Res

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Foot-and-mouth disease (FMD) is a highly contagious and economically devastating disease of cloven-hoofed animals in the world. The disease can be effectively controlled by vaccination of susceptible animals with the conventional inactivated vaccine. However, one major concern of the inactivated FMD virus (FMDV) vaccine is that it does not allow serological discrimination between infected and vaccinated animals, and therefore interferes with serologic surveillance and the epidemiology of disease. A marker vaccine has proven to be of great value in disease eradication and control programs. In this study, we constructed a marker FMDV containing a deletion of residues 93 to 143 in the nonstructural protein 3A using a recently developed FMDV infectious cDNA clone. The marker virus, r-HN/3A93–143, had similar growth kinetics as the wild type virus in culture cell and caused a symptomatic infection in pigs. Pigs immunized with chemically inactivated marker vaccine were fully protected from the wild type virus challenge, and the potency of this marker vaccine was 10 PD50 (50% pig protective dose) per dose, indicating it could be an efficacious vaccine against FMDV. In addition, we developed a blocking ELISA targeted to the deleted epitope that could clearly differentiate animals infected with the marker virus from those infected with the wild type virus. These results indicate that a marker FMDV vaccine can be potentially developed by deleting an immunodominant epitope in NSP 3A.

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Marker vaccine potential of a foot-and-mouth disease virus with a partial VP1 G-H loop deletion

Cited by 40 publications

References 29 publications

A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools

A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools

Xenoepitope Substitution Avoids Deceptive Imprinting and Broadens the Immune Response to Foot-and-Mouth Disease Virus

Evaluation of a 3A-truncated foot-and-mouth disease virus in pigs for its potential as a marker vaccine

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