2011
DOI: 10.4061/2011/905768
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A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools

Abstract: Foot-and-mouth disease (FMD) is one of the highly contagious diseases of domestic animals. Effective control of this disease needs sensitive, specific, and quick diagnostic tools at each tier of control strategy. In this paper we have outlined various diagnostic approaches from old to new generation in a nutshell. Presently FMD diagnosis is being carried out using techniques such as Virus Isolation (VI), Sandwich-ELISA (S-ELISA), Liquid-Phase Blocking ELISA (LPBE), Multiplex-PCR (m-PCR), and indirect ELISA (DI… Show more

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Cited by 95 publications
(86 citation statements)
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References 180 publications
(239 reference statements)
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“…The enzyme linked immunosorbent assay (ELISA) has now largely replaced serum neutralization test (SNT) for routine testing of animal sera for antibodies (Armstrong and Barnett, 1989) Antibodies to capsid proteins (structural proteins) are manufacture from animals either infected or vaccinated but antibodies to nonstructural proteins (NSP) are only manufacture from infected one (Rodriguez and Gay, 2011). So, 3ABc-ELISA (The most reliable indicator for NSP) is highly suitable for large scale testing and can therefore be adapted for monitoring and eradication programs as it allows differentiation between infected and vaccinated animals (Longjam et al, 2011) .…”
Section: Introductionmentioning
confidence: 99%
“…The enzyme linked immunosorbent assay (ELISA) has now largely replaced serum neutralization test (SNT) for routine testing of animal sera for antibodies (Armstrong and Barnett, 1989) Antibodies to capsid proteins (structural proteins) are manufacture from animals either infected or vaccinated but antibodies to nonstructural proteins (NSP) are only manufacture from infected one (Rodriguez and Gay, 2011). So, 3ABc-ELISA (The most reliable indicator for NSP) is highly suitable for large scale testing and can therefore be adapted for monitoring and eradication programs as it allows differentiation between infected and vaccinated animals (Longjam et al, 2011) .…”
Section: Introductionmentioning
confidence: 99%
“…The epidemiology of FMD is complex, and it is affected by different viral, host, and environmental factors (Longjam et al, 2011 …”
Section: Introductionmentioning
confidence: 99%
“…A variety of strains is one of the difficulties in controlling FMDV outbreak, since a vaccine for one specific serotype will not protect against any of the others, and vaccination only provides temporary immunity [4][5][6]. Slaughter of infected animals is therefore another significant control option in unavoidable cases, and can cause serious economic problems [4,7].…”
Section: Introductionmentioning
confidence: 99%