Marker-ion analysis for quantification of mucoadhesivity of microparticles in particle-retention assays

Preisig, Daniel; Weingärtner, Michael; Varum, Felipe; Bravo, Roberto; Alles, Rainer; Huwyler, Jörg

doi:10.1016/j.ijpharm.2015.04.020

Cited by 6 publications

(2 citation statements)

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“…Moreover, the mucoadhesiveness of the vaterite microparticles has been reported. [ 26 ] The incorporation of various enzymes into the calcium carbonate (CC) nano‐ and microspheres by means of adsorption and co‐precipitation has been proposed for ocular delivery, [ 27 ] for subsequent production of polymer microcapsules by layer‐by‐layer deposition of polyelectrolytes, and for the creation of biocatalysts. [ 28–30 ]…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the mucoadhesiveness of the vaterite microparticles has been reported. [26] The incorporation of various enzymes into the calcium carbonate (CC) nano-and microspheres by means of adsorption and co-precipitation has been proposed for ocular delivery, [27] for subsequent production of polymer microcapsules by layer-by-layer deposition of polyelectrolytes, and for the creation of biocatalysts. [28][29][30] In recent years, hybrid forms of vaterite containing natural polymers (alginate, [31] chitosan and its derivatives, [32][33][34] cellulose and its derivatives, [35,36] heparin, [37] globular proteins, [38] etc.)…”

Section: Introductionmentioning

confidence: 99%

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Hybrid Mucin‐Vaterite Microspheres for Delivery of Proteolytic Enzyme Chymotrypsin

Балабушевич

Коваленко

Filatova

et al. 2022

Macromolecular Bioscience

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While the enteral delivery of proteolytic enzymes is widely established for combating many diseases as an alternative to antibiotic treatment, their local delivery only emerges as administration route enabling sustained release in a controlled manner on site. The latest requires the development of drug delivery systems suitable for encapsulation and preservation of enzymatic proteolytic activity. This study proposes hybrid microspheres made of mucin and biodegradable porous crystals of calcium carbonate (CC) as the carriers for chymotrypsin (CTR) delivery. CTR is impregnated into CC and hybrid CC/mucin (CCM) microspheres by means of sorption without any chemical modification. The loading of the CC with mucin enhances CTR retention on hybrid microspheres (adsorption capacity of ≈8.7 mg g −1 vs 4.7 mg g −1 ), recharging crystal surface due to the presence of mucin and diminishing the average pore diameter of the crystals from 25 to 8 nm. Mucin also retards recrystallization of vaterite into nonporous calcite improving stability of CCM microspheres upon storage. Proteolytic activity of CTR is preserved in both CC and CCM microspheres, being pH dependent. Temperature-induced inactivation of CTR significantly diminishes by CTR encapsulation into CC and CCM microspheres. Altogether, these findings indicate promises of hybrid mucin-vaterite microspheres for mucosal application of proteases.

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Section: Introductionmentioning

confidence: 99%