Marine Collagen Isolation and Processing Envisaging Biomedical Applications

Moreira‐Silva, Joana; Diogo, Gabriela S.; Marques, Ana Paula; Silva, Tiago H.; Reis, Rui L.

doi:10.1002/9781119126218.ch2

Cited by 16 publications

(25 citation statements)

References 69 publications

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“…Equally important and regarding their great biotechnological potential, cod by-products can be established as alternative raw-materials for the production of biomolecules highly demanded by several industrial sectors. In particular, cod skin is a significant by-product generated when cod is processed as fillets and/or shredded, which could be utilized to produce collagen [5][6][7][8], often incorporated in the production of functional food or medicine due to its properties.…”

Section: Introductionmentioning

confidence: 99%

Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare

et al. 2020

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The extraction of collagen from fish skins is being proposed as strategy for valorization of marine origin by-products, being a sustainable alternative to mammal collagen. The method commonly uses solutions of organic acids, but new methodologies are arising, aiming to improve process yields and/or the properties of the resulting products. In this work, skins removed from salt brine Atlantic cod (Gadus morhua) were used to extract collagen, using water acidified with CO 2 , obtaining an extraction yield of 13.8% (w/w). Acidified water extracted collagen (AWC) presented a total content of proline-like amino acids of 151/1000 residues, with a degree of hydroxylation of 38%, and its SDS-PAGE profile is compatible with type I collagen. Moreover, FTIR, CD and XRD results suggest the presence of preserved triple helix, having a denaturation temperature of 32.3°C as determined by micro-DSC. AWC exhibited a typical shear thinning behavior, interesting regarding their further processing, namely in jelly-like formulations. Additionally, the presence of AWC in MRC-5 human fibroblasts culture did not affect cell viability, demonstrating the non-cytotoxic behavior. Overall, the results support the efficiency of the proposed approach for collagen extraction and further enable the design of methodologies to address AWC use in biomedical or cosmetic context.

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Section: Introductionmentioning

confidence: 99%

Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare

et al. 2020

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“…Considering its favorable features and the central role that collagen assumes in extracellular matrix, it has been widely proposed for biomedical application, namely the types I to V [28,29], with marine origin collagen being consistent with collagen type I (fish skins, scales and bones), type II (jellyfish, fish cartilage) or type IV (sea sponges) [27]. In particular, the use of collagen on the development of biomaterials mimicking extracellular matrix in tissue engineering approaches is a hot topic [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Extraction and characterization of collagen from Antarctic and Sub-Antarctic squid and its potential application in hybrid scaffolds for tissue engineering

Coelho

Marques

Oliveira

et al. 2017

Materials Science and Engineering: C

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Collagen is the most abundant protein found in mammals and it exhibits a low immunogenicity, high biocompatibility and biodegradability when compared with others natural polymers. For this reason, it has been explored for the development of biologically instructive biomaterials with applications for tissue substitution and regeneration. Marine origin collagen has been pursued as an alternative to the more common bovine and porcine origins. This study focused on squid (Teuthoidea: Cephalopoda), particularly the Antarctic squid Kondakovia longimana and the Sub-Antarctic squid Illex argentinus as potential collagen sources. In this study, collagen has been isolated from the skins of the squids using acid-based and pepsin-based protocols, with the higher yield being obtained from I. argentinus in the presence of pepsin. The produced collagen has been characterized in terms of physicochemical properties, evidencing an amino acid profile similar to the one of calf collagen, but exhibiting a less preserved structure, with hydrolyzed portions and a lower melting temperature. Pepsin-soluble collagen isolated from I. argentinus was selected for further evaluation of biomedical potential, exploring its incorporation on poly-ε-caprolactone (PCL) 3D printed scaffolds for the development of hybrid scaffolds for tissue engineering, exhibiting hierarchical features.

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“…Collagen extraction/Physical form after extraction Marine collagens, predominantly type I collagen, can be isolated from marine invertebrates (sponges, jellyfish, cephalopods, echinoderms) and marine vertebrates (fish) [176,254,255]. The raw materials for fish collagen isolation include skin, scale, fins, backbone, swimbladder, wing muscles of skate, shark placoid-scale dentine [230,254].…”

Section: Scientific Name Collagensmentioning

confidence: 99%

“…Marine collagen is extracted via (i) decellularization using physical methods involving freezing and disruption of cells; (ii) chemical methods based on variable reagents, i.e., acids, alkalis, chelating agents, detergents, solutions of high osmolarity; (iii) enzymatic treatments. Usually, these methods are combined [230,255]. From jellyfish, it is extracted from mesoglea via solubilization in acetic acid solution [256].…”

Section: Scientific Name Collagensmentioning

confidence: 99%

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Progress in Modern Marine Biomaterials Research

et al. 2020

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The growing demand for new, sophisticated, multifunctional materials has brought natural structural composites into focus, since they underwent a substantial optimization during long evolutionary selection pressure and adaptation processes. Marine biological materials are the most important sources of both inspiration for biomimetics and of raw materials for practical applications in technology and biomedicine. The use of marine natural products as multifunctional biomaterials is currently undergoing a renaissance in the modern materials science. The diversity of marine biomaterials, their forms and fields of application are highlighted in this review. We will discuss the challenges, solutions, and future directions of modern marine biomaterialogy using a thorough analysis of scientific sources over the past ten years.

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Marine Collagen Isolation and Processing Envisaging Biomedical Applications

Cited by 16 publications

References 69 publications

Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare

Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare

Extraction and characterization of collagen from Antarctic and Sub-Antarctic squid and its potential application in hybrid scaffolds for tissue engineering

Progress in Modern Marine Biomaterials Research

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