Mapping the gaps in chemical analysis for the characterisation of aptamer-target interactions

Daems, Elise; Moro, Giulia; Campos, Rui; Wael, Karolien De

doi:10.1016/j.trac.2021.116311

Cited by 42 publications

(46 citation statements)

References 146 publications

(181 reference statements)

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“…Labels are employed in electrochemical biosensors to boost the signal produced per occurrence. Electrochemical labelling often involves chemical methods that covalently bind labels, but some probe labelling merely requires momentary (physical-binding) attachments [ 75 , 76 , 77 ]. In one of the fascinating studies, Karash et al designed an impedance-based aptasensor utilizing a specific avian influenza virus (AIV) H5N1 aptamer and a gold interdigitated microelectrode.…”

Section: Label and Label-free Aptasensorsmentioning

confidence: 99%

Ultrasensitive Aptasensors for the Detection of Viruses Based on Opto-Electrochemical Readout Systems

et al. 2022

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Viral infections are becoming the foremost driver of morbidity, mortality and economic loss all around the world. Treatment for diseases associated to some deadly viruses are challenging tasks, due to lack of infrastructure, finance and availability of rapid, accurate and easy-to-use detection methods or devices. The emergence of biosensors has proven to be a success in the field of diagnosis to overcome the challenges associated with traditional methods. Furthermore, the incorporation of aptamers as bio-recognition elements in the design of biosensors has paved a way towards rapid, cost-effective, and specific detection devices which are insensitive to changes in the environment. In the last decade, aptamers have emerged to be suitable and efficient biorecognition elements for the detection of different kinds of analytes, such as metal ions, small and macro molecules, and even cells. The signal generation in the detection process depends on different parameters; one such parameter is whether the labelled molecule is incorporated or not for monitoring the sensing process. Based on the labelling, biosensors are classified as label or label-free; both have their significant advantages and disadvantages. Here, we have primarily reviewed the advantages for using aptamers in the transduction system of sensing devices. Furthermore, the labelled and label-free opto-electrochemical aptasensors for the detection of various kinds of viruses have been discussed. Moreover, numerous globally developed aptasensors for the sensing of different types of viruses have been illustrated and explained in tabulated form.

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Section: Label and Label-free Aptasensorsmentioning

confidence: 99%

Ultrasensitive Aptasensors for the Detection of Viruses Based on Opto-Electrochemical Readout Systems

et al. 2022

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“…The study of the binding kinetics and thermodynamics, along with the structural features of the peptidetarget complex, is instrumental in evaluating the applicability of the selected peptide as a bioreceptor and, eventually, the identification of the most suitable design for the sensing platform. The design of the peptide-target characterisation protocol should take into account the final application of the peptide sequence (i.e., the biosensing platform) as the binding performance can vary depending on the physical and chemical properties of the surrounding environment, as recently discussed for other biorecognition elements [32]. Characterisation of the peptide-target complex is therefore fundamental to the evaluation of the peptide's applicability, the discrimination of a restricted pool of selected sequences, and, eventually, the consideration to improve biorecognition elements, for instance, by using peptides in conjugation with other biomolecules [33,34].…”

Section: Peptide Selection Synthesis and Characterisationmentioning

confidence: 99%

The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics

et al. 2021

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Peptides represent a promising class of biorecognition elements that can be coupled to electrochemical transducers. The benefits lie mainly in their stability and selectivity toward a target analyte. Furthermore, they can be synthesized rather easily and modified with specific functional groups, thus making them suitable for the development of novel architectures for biosensing platforms, as well as alternative labelling tools. Peptides have also been proposed as antibiofouling agents. Indeed, biofouling caused by the accumulation of biomolecules on electrode surfaces is one of the major issues and challenges to be addressed in the practical application of electrochemical biosensors. In this review, we summarise trends from the last three years in the design and development of electrochemical biosensors using synthetic peptides. The different roles of peptides in the design of electrochemical biosensors are described. The main procedures of selection and synthesis are discussed. Selected applications in clinical diagnostics are also described.

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“…The interaction between an aptamer and its target is the core of the SELEX process and practical application. The nature of this interaction depends on the type and size of the target [143]. When the target is a small molecule, the aptamer can integrate the target into its structure through stacking (with flat, aromatic ligands and ions), electrostatic complementation (with oligosaccharides and charged amino acids), and/or hydrogen bonding interactions [144].…”

Section: Aptamer-target Interaction Mechanismsmentioning

confidence: 99%

Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices

Hong

Pan

Xie

et al. 2021

Foods

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Food allergies have seriously affected the life quality of some people and even endangered their lives. At present, there is still no effective cure for food allergies. Avoiding the intake of allergenic food is still the most effective way to prevent allergic diseases. Therefore, it is necessary to develop rapid, accurate, sensitive, and reliable analysis methods to detect food allergens from different sources. Aptamers are oligonucleotide sequences that can bind to a variety of targets with high specificity and selectivity, and they are often combined with different transduction technologies, thereby constructing various types of aptamer sensors. In recent years, with the development of technology and the application of new materials, the sensitivity, portability, and cost of fluorescence sensing technology have been greatly improved. Therefore, aptamer-based fluorescence sensing technology has been widely developed and applied in the specific recognition of food allergens. In this paper, the classification of major allergens and their characteristics in animal and plant foods were comprehensively reviewed, and the preparation principles and practical applications of aptamer-based fluorescence biosensors are summarized. In addition, we hope that this article can provide some strategies for the rapid and sensitive detection of allergens in food matrices.

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Mapping the gaps in chemical analysis for the characterisation of aptamer-target interactions

Cited by 42 publications

References 146 publications

Ultrasensitive Aptasensors for the Detection of Viruses Based on Opto-Electrochemical Readout Systems

Ultrasensitive Aptasensors for the Detection of Viruses Based on Opto-Electrochemical Readout Systems

The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics

Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices

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