The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics

Sfragano, Patrick Severin; Moro, Giulia; Polo, Federico; Palchetti, Ilaria

doi:10.3390/bios11080246

Cited by 53 publications

(36 citation statements)

References 116 publications

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“…In general, the detection occurs through fluorescence changes related to the peptide motif or colorimetry based on nanoparticle properties [164,181]. The recognition unit was initially based on antibodies [182], but less problematic protein aptamers [183], and, finally, regular peptides [184] are now used. In some cases, due to the competition between the nanoparticle and the analyte for the peptide probe, the spectroscopic or catalytic activities of nanoparticles are revealed to provide a detectable signal [106,183].…”

Section: Discussion: Peptide Biosensors-advantages and Applicationsmentioning

confidence: 99%

Veni, Vidi, Vici: Immobilized Peptide-Based Conjugates as Tools for Capture, Analysis, and Transformation

et al. 2022

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Analysis of peptide biomarkers of pathological states of the organism is often a serious challenge, due to a very complex composition of the cell and insufficient sensitivity of the current analytical methods (including mass spectrometry). One of the possible ways to overcome this problem is sample enrichment by capturing the selected components using a specific solid support. Another option is increasing the detectability of the desired compound by its selective tagging. Appropriately modified and immobilized peptides can be used for these purposes. In addition, they find application in studying the specificity and activity of proteolytic enzymes. Immobilized heterocyclic peptide conjugates may serve as metal ligands, to form complexes used as catalysts or analytical markers. In this review, we describe various applications of immobilized peptides, including selective capturing of cysteine-containing peptides, tagging of the carbonyl compounds to increase the sensitivity of their detection, enrichment of biological samples in deoxyfructosylated peptides, and fishing out of tyrosine–containing peptides by the formation of azo bond. Moreover, the use of the one-bead-one-compound peptide library for the analysis of substrate specificity and activity of caspases is described. Furthermore, the evolution of immobilization from the solid support used in peptide synthesis to nanocarriers is presented. Taken together, the examples presented here demonstrate immobilized peptides as a multifunctional tool, which can be successfully used to solve multiple analytical problems.

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Section: Discussion: Peptide Biosensors-advantages and Applicationsmentioning

confidence: 99%

Veni, Vidi, Vici: Immobilized Peptide-Based Conjugates as Tools for Capture, Analysis, and Transformation

et al. 2022

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“… 18 , 19 Thus, peptides have been widely used to develop point-of-care diagnosis platforms. 20 , 21 It is noteworthy that, even though the detection sensitivity of the direct detection using a peptide assay is lower than nucleic acid assays such as PCR, the peptide-based direct detection method without the needs for viral lysis, extraction, and amplification will be useful to diagnose the patients, who already display symptoms and present a high viral load. 22 , 23 …”

Section: Introductionmentioning

confidence: 99%

Phage Display-Derived Peptide for the Specific Binding of SARS-CoV-2

et al. 2021

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Beginning from the end of 2019, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic swept all over the world and is still afflicting the whole global population. Given that the vaccine-manufacturing ability is limited and the virus can evolve quickly, vaccination alone may not be able to end the pandemic, thus developing fast and accurate diagnoses and effective therapeutics will always be unmet needs. Phage display peptide library has been used in screening antigen-specific peptides for the invention of novel mimic receptors/ligands. Here, we report that a 12-mer phage display peptide library has been screened against the SARS-CoV-2 receptor-binding domain (RBD), and five of the screened peptides show binding ability with the RBD protein by the enzyme-linked immune sorbent assay. The surface plasmon resonance assay further demonstrates that peptide no. 1 can specifically bind to SARS-CoV-2 RBD with a binding affinity constant ( K d ) of 5.8 μM. Transmission electron microscopy coupled with a magnetic bead assay further confirms that the screened peptide can specifically bind the inactivated SARS-CoV-2 virus. This SARS-CoV-2-specific peptide holds great promise as a new bioreceptor/ligand for the rapid and accurate detection of SARS-CoV-2.

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“…Similarly to aptamers, peptides (amino acid sequences of different length and weight comprising synthetic or natural amino acids connected by peptide bonds), are experiencing an unstoppable rise in developing high performance electrochemical biosensors. [1,6] Peptides are promising affinity biorecognition elements that, in connection with electrochemical transducers, provide advantages primarily in terms of stability, selectivity, structural diversity, possibility of tuning the amino acid sequence, and biocompatibility. These recognition elements of synthetic nature can be easily obtained with high yield and functionalized with specific groups (affinity or electroactive tags) for binding (thiol, biotin, etc.)…”

Section: Modern Peptidesmentioning

confidence: 99%

“…and, as enzyme substrates, play an irreplaceable role to determine the activity (or inhibiting) of clinically relevant enzymes such as proteases (the peptide serves as cleavage-sensing element) and kinases (catalyzed phosphorylation reactions). [1,6] Because of their distinguished properties and the opportunities they impart to the final devices, this section discusses also the use of antifouling, multifunctional, phage-displayed, and aberrant peptides. The characteristics and performance of the selected biosensors are summarized in Table 3.…”

Section: Modern Peptidesmentioning

confidence: 99%

Empowering Electrochemical Biosensing through Nanostructured or Multifunctional Nucleic Acid or Peptide Biomaterials

Campuzano

Pedrero

Barderas

et al. 2022

Adv Materials Technologies

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Electrochemical biosensors continue to evolve at an astonishing pace, consolidating as competitive tools for determining a wide range of targets and relentlessly strengthening their attributes in terms of sensitivity, selectivity, simplicity, response time, and antifouling ability, making them suitable for getting a foothold in real‐world applications. The design and exploitation of nanostructured or multifunctional nucleic acid or peptide biomaterials are playing a determinant role in these achievements. With the aim of highlighting the potential and opportunities of these biomaterials, this perspective article critically discusses and overviews the electrochemical biosensors reported since 2019 involving nanostructured and multifunctional DNA biomaterials, multifunctional aptamers, modern peptides, and CRISPR/Cas systems. The use of these biomaterials as recognition elements, electrode modifiers (acting as linkers or creating scaffolds with antifouling properties), enzyme substrates, and labeling/carrier agents for signal amplification is discussed through rationally and strategically selected examples, concluding with a personal perspective about the challenges to be faced and future lines of action.

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The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics

Cited by 53 publications

References 116 publications

Veni, Vidi, Vici: Immobilized Peptide-Based Conjugates as Tools for Capture, Analysis, and Transformation

Veni, Vidi, Vici: Immobilized Peptide-Based Conjugates as Tools for Capture, Analysis, and Transformation

Phage Display-Derived Peptide for the Specific Binding of SARS-CoV-2

Empowering Electrochemical Biosensing through Nanostructured or Multifunctional Nucleic Acid or Peptide Biomaterials

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