Mapping of the SLA complex class III region by pulsed field gel electrophoresis

Wu, Lizi; Rothschild, Max F.; Warner, Carol M.

doi:10.1007/bf00352366

Cited by 6 publications

(2 citation statements)

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“…We should also mention that in the mouse H-2 d complex the Bat-1 gene is duplicated in association with the D a and L d class I genes (Wroblewski et al 1994), whereas in pig and human only one BAT1 gene seems to exist. The comparison of our PGFE data with those published by others in miniature pigs (Xu et al 1992;Wu et al 1995) are generally in agreement, with some differences with a few restriction enzymes, which may indicate restriction enzyme site polymorphisms between miniature and Large White breeds. The distance between the class II region (DRA) and the class III region, which is about 200 kb in length, seems to be conserved between humans, pigs, and mice (Lunney 1994).…”

Section: Discussionsupporting

confidence: 90%

A detailed physical map of the porcine major histocompatibility complex (MHC) class III region: Comparison with human and mouse MHC class III regions

et al. 1996

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A detailed physical map of the porcine MHC class III region on Chr 7 was constructed with a panel of probes in a series of hybridizations on genomic pulsed field gel electrophoresis (PFGE) Southern blots. A precise organization of the 700-kb segment of DNA between G18 and BAT1 can now be proposed, with more than 30 genes mapped to it. Comparison of this region with homologous regions in human and mouse showed only minor differences. The biggest difference was observed in the CYP21/C4 locus with only one CYP21 gene and one C4 gene found, whereas in human and mouse these genes are duplicated. These results show the class III region is very well conserved between pig, human, and mouse, in contrast with the class I and class II regions, which seem more prone to rearrangements.

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Section: Discussionsupporting

confidence: 90%

A detailed physical map of the porcine major histocompatibility complex (MHC) class III region: Comparison with human and mouse MHC class III regions

et al. 1996

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“…The swine MHC region also comprises three closely linked Hsp70 loci which have been mapped to the class III region [34,35,50]. Linkage of the Hsp70 gene with MHC has also been reported in the goat and cattle [5,13].…”

Section: Discussionmentioning

confidence: 99%

Expression of the Spermatid-Specific Hsp70 Antigen is Conserved in Mammals Including Marsupials

Tsunekawa

Nishida

Fujimoto

1999

The Journal of Veterinary Medical Science

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ABSTRACT. The anatomical location of testes in mammals ranges from a location close to that observed in the embryo to a lower position usually involving a pendant scrotum. In scrotal mammals, the abdominal position of the cryptorchid testis, which elevates its temperature, is detrimental to spermatogenesis and causes infertility. Spermatocytes are sensitive but late spermatids are relatively resistant to thermal stress suggesting that the latter might be protected in some way. In general, most organisms express Hsp70 proteins, which play a crucial role in the protection of cells against thermal stress. We have found previously that the Hsc70t protein, a member of the Hsp70 family of proteins, is constitutively expressed in the late spermatids of mice. Here, we have utilized immunohistochemistry with anti-mouse Hsc70t antiserum to examine the expression of the spermatid-specific Hsp70 antigen in the testes of several mammalian species with different degrees of testes migration. Our data indicate that the antigen is conserved in the mammals including marsupials. We also examined whether antigens of Hsp70-related proteins were expressed in non-mammalian vertebrates including not only homoiothermal but also poikilothermal animals. The spermatid-specific Hsp70 antigens were not detectable in the testes of the animals examined. From results of immunohistochemistry with BRM22 monoclonal antibody which reacts broadly with Hsp70 family proteins, however, we revealed constitutive expression of antigens of Hsp70-related proteins in spermatogenic cells of the vertebrates. These results suggest that the expression of spermatid-specific Hsp70 protein may be involved in the developmental pathway during spermiogenesis in mammals rather than in thermotolerance.-KEY WORDS: Hsp70, immunohistochemistry, mammal, spermatogenesis, testis migration.J. Vet. Med. Sci. 61(4): [381][382][383][384][385][386][387][388] 1999 prominent and evolutionary well conserved is the 70 kDa heat shock protein (Hsp70) [26]. Some role of the Hsp70 in cellular response to heat injury is suggested by the temporal correlation between Hsp70 synthesis and the appearance of thermotolerance [22,23,43] and the increased sensitivity to heat when intracellular Hsp70 levels are reduced by antiHsp70 antibodies [38] or by competitive inhibition of Hsp70 mRNA [18]. Most eukaryotes have a large family of at least five Hsp70-related proteins [15,16,32]. A unique pattern of expression of Hsp70 has been revealed during mouse and rat spermatogenesis [2,19,20]. Mouse Hsp70-2 has been shown to be expressed at high levels during meiosis [41]. Extensive analyses using knock-out mutant mice of the Hsp70-2 gene revealed that Hsp70-2 is required for synaptonemal complex desynapsis [1,9,10,51]. We have showed that another kind of Hsp70-related protein, Hsc70t, is expressed in late spermatids during spermiogenesis [27,29,45]. The amino acid sequences of mouse Hsc70t gene and human orthologous gene HSPA1L are highly similar to those of heat-inducible Hsp70 genes, but these genes d...

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Physical Mapping of the Mouse Genome

Herman

1998

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Mapping of the SLA complex class III region by pulsed field gel electrophoresis

Cited by 6 publications

References 27 publications

A detailed physical map of the porcine major histocompatibility complex (MHC) class III region: Comparison with human and mouse MHC class III regions

A detailed physical map of the porcine major histocompatibility complex (MHC) class III region: Comparison with human and mouse MHC class III regions

Expression of the Spermatid-Specific Hsp70 Antigen is Conserved in Mammals Including Marsupials

Physical Mapping of the Mouse Genome

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