A detailed physical map of the porcine major histocompatibility complex (MHC) class III region: Comparison with human and mouse MHC class III regions

Peelman, Luc; Chardon, Patrick; Vaiman, M.; Mattheeuws, Marc; Zeveren, Alex Van; Weghe, Alex Van De; Bouquet, Y.; Campbell, R. Duncan

doi:10.1007/s003359900105

Cited by 32 publications

(17 citation statements)

References 36 publications

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“…The gene order within the ELA class III region appears to be well conserved with class III regions of human, mouse, and pig, which is consistent with the suggestion that this region of the mammalian genome is evolutionarily more conserved than the other regions of the MHC (Peelman et al, 1996). This conservation is evidence of a potential functional significance related to the organization of these genes within the MHC region.…”

Section: Discussionsupporting

confidence: 72%

An ordered BAC contig map of the equine major histocompatibility complex

Gustafson

Tallmadge

Ramlachan

et al. 2003

Cytogenet Genome Res

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A physical map of ordered bacterial artificial chromosome (BAC) clones was constructed to determine the genetic organization of the horse major histocompatibility complex. Human, cattle, pig, mouse, and rat MHC gene sequences were compared to identify highly conserved regions which served as source templates for the design of overgo primers. Thirty-five overgo probes were designed from 24 genes and used for hybridization screening of the equine USDA CHORI 241 BAC library. Two hundred thirty-eight BAC clones were assembled into two contigs spanning the horse MHC region. The first contig contains the MHC class II region and was reduced to a minimum tiling path of nine BAC clones that span approximately 800 kb and contain at least 20 genes. A minimum tiling path of a second contig containing the class III/I region is comprised of 14 BAC clones that span approximately 1.6 Mb and contain at least 34 genes. Fluorescence in situ hybridization (FISH) using representative clones from each of the three regions of the MHC localized the contigs onto ECA20q21 and oriented the regions relative to one another and the centromere. Dual-colored FISH revealed that the class I region is proximal to the centromere, the class II region is distal, and the class III region is located between class I and II. These data indicate that the equine MHC is a single gene-dense region similar in structure and organization to the human MHC and is not disrupted as in ruminants and pigs.

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Section: Discussionsupporting

confidence: 72%

An ordered BAC contig map of the equine major histocompatibility complex

Gustafson

Tallmadge

Ramlachan

et al. 2003

Cytogenet Genome Res

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“…Locus order specified for the CLIII haplotype was based on the physical data presented by Peelman et al (1996). No recombinant genotypes were identified within the CLI, CLII, or PI2/PO1 A regions, allowing the 'hap_sys0' option to be implemented.…”

Section: Methodsmentioning

confidence: 99%

A consensus linkage map for swine chromosome 7

Rohrer¹,

Alexander²,

Beattie

et al. 1997

Animal Genetics

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objective was to develop a comprehensive linkage map for porcine chromosome 7 by combining genotypic data from four swine reference populations. Contributions of genotypic data were made from the US Meat Animal Research Center, the University of Minnesota, the PiGMaP consortium and the Scandinavian consortium. Primers for selected sequence tagged site markers, to be genotyped across the reference populations, were exchanged to integrate individual maps of SSC7. Eighty-six loci were genotyped; these loci represented microsatellite, minisatellite, single-strand conformation polymorphism, restriction fragment length polymorphism, erythrocyte antigen and protein polymorphisms. Eighteen genes were mapped, including 12 markers within class I, class II and class III regions (four markers in each class) of the swine major histocompatibility complex. Forty-two markers were either genotyped on more than one population or were included in a haplotype system and used to develop skeletal linkage maps that spanned 147·6, 212·7 and 179·5 cM for the male, female and sex-average maps, respectively. A comprehensive linkage map was developed incorporating those markers with more than 30 informative meioses. The comprehensive map was slightly longer than the skeletal map, at 153·3, 215·3 and 183·8 cM, respectively, with only three intervals greater than 10 cM. These results significantly improve the genetic resolution of the porcine chromosome 7 map and represent an accurate approach for the merging of genetic maps produced in different reference populations.

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“…Taken together, we have localized the bovine homologs of 21 out of 60 human class III genes in the bovine class III region and found it well conserved with the class III regions of human, mouse, pig and horse (Peelmann et al, 1996;Gustafson et al, 2003;Xie et al, 2003). Ten of these genes have been assigned to bovine chromosome 23 for the first time ( Table 1 , 2 ).…”

Section: Y-h8237mentioning

confidence: 96%

YAC/BAC contig spanning the MHC class III region of cattle

Gelhaus

Hess²,

Förster³

et al. 2006

Cytogenet Genome Res

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A contig of the class III region of the bovine major histocompatibility complex (MHC) was established from bacterial and yeast artificial chromosomes using PCR and BAC-end sequencing. The marker content of individual clones was determined by gene and BAC-end specific PCR, and the location of genes and BAC-ends was confirmed analyzing somatic hybrid cells. A comparative analysis indicated that the content and order of MHC class III genes is strongly conserved between cattle and other mammalian species. Fluorescence in situ hybridization localized the bovine class III region to BTA23q21→q22. The results show that the collection of sequenced BAC-ends is a powerful resource for generating high-resolution comparative chromosome maps.

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A detailed physical map of the porcine major histocompatibility complex (MHC) class III region: Comparison with human and mouse MHC class III regions

Cited by 32 publications

References 36 publications

An ordered BAC contig map of the equine major histocompatibility complex

An ordered BAC contig map of the equine major histocompatibility complex

A consensus linkage map for swine chromosome 7

YAC/BAC contig spanning the MHC class III region of cattle

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