Mapping of the root lesion nematode (Pratylenchus neglectus) resistance gene Rlnn1 in wheat

Williams, Kevin Jon; Taylor, S.; Bogacki, P.; Pallotta, Margaret; Bariana, Harbans; Wallwork, H.

doi:10.1007/s00122-001-0839-3

Cited by 83 publications

(66 citation statements)

References 20 publications

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“…These QTLs identified in the CPI133872 × Janz population are all novel regions associated with resistance to P. neglectus. In comparison, the only previously mapped P. neglectus resistance gene (Rlnn1) was reported as a monogenic trait located on chromosome 7AL in the wheat cultivar Excalibur (Williams et al 2002). However, no marker-trait associations were detected with markers on 7AL in the CPI133872 × Janz population.…”

Section: Discussionmentioning

confidence: 84%

“…Chromosome regions with putative associations to resistance to P. thornei and P. neglectus were then targetted with additional microsatellite markers to increase map density in these areas of interest. The PCR marker Xschfc3 (Williams et al 2002) was screened across the entire mapping population using conditions as described above for microsatellite analysis. Chi-square analyses were applied to test the goodness-of-fit of the observed segregation ratios to theoretical expectations of the Mendelian segregation ratio (1 : 1).…”

Section: Bsa and Framework Mapmentioning

confidence: 99%

“…QTLs for resistance to P. thornei were identified in the International Triticeae Mapping Initiative (ITMI) population on chromosomes 6D and 2B (Thompson et al 1999). A single gene for P. neglectus resistance, Rlnn1, has been mapped to chromosome 7AL in a Tammin × Excalibur cross (Williams et al 2002). Markers linked to Rlnn1 in this population were the microsatellite marker Xgwm344, the flour colour PCR marker Xschfc3, co-segregating restriction fragment length polymorphism (RFLP) markers Xcdo347, Xpsr680A, and Xpsr121, and AFLP marker XAGC/CCT179 (Williams et al 2002).…”

Section: Introductionmentioning

confidence: 99%

“…A single gene for P. neglectus resistance, Rlnn1, has been mapped to chromosome 7AL in a Tammin × Excalibur cross (Williams et al 2002). Markers linked to Rlnn1 in this population were the microsatellite marker Xgwm344, the flour colour PCR marker Xschfc3, co-segregating restriction fragment length polymorphism (RFLP) markers Xcdo347, Xpsr680A, and Xpsr121, and AFLP marker XAGC/CCT179 (Williams et al 2002). Identification of novel sources of resistance and further understanding of the genetics of different sources of resistance will enable combinations of resistance genes to be pyramided, producing wheat varieties with durable multiple disease resistance.…”

Section: Introductionmentioning

confidence: 99%

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Identification of quantitative trait loci for resistance to two species of root-lesion nematode (Pratylenchus thornei and P. neglectus) in wheat

Zwart

Thompson²,

Godwin

2005

Aust. J. Agric. Res.

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Abstract. Pratylenchus thornei and P. neglectus are two species of root-lesion nematode that cause substantial yield losses in wheat. No commercially available wheat variety has resistance to both species. A doubled-haploid population developed from a cross between the synthetic hexaploid wheat line CPI133872 and the bread wheat Janz was used to locate and tag quantitative trait loci (QTLs) associated with resistance to both P. thornei and P. neglectus. Wheat plants were inoculated with both species of nematode in independent replicated glasshouse trials repeated over 2 years. Known locations of wheat microsatellite markers were used to construct a framework map. After an initial single-marker analysis to detect marker-trait linkages, chromosome regions associated with putative QTLs were targetted with microsatellite markers to increase map density in the chromosome regions of interest. In total, 148 wheat microsatellite markers and 21 amplified fragment length polymorphism markers were mapped. The codominant microsatellite marker Xbarc183 on the distal end of chromosome 6DS was allelic for resistance to both P. thornei and P. neglectus. The QTL were designated QRlnt.lrc-6D.1 and QRlnn.lrc-6D.1, for the 2 traits, respectively. The allele inherited from CPI133872 explained 22.0-24.2% of the phenotypic variation for P. thornei resistance, and the allele inherited from Janz accounted for 11.3-14.0% of the phenotypic variation for P. neglectus resistance. Composite interval mapping identified markers that flank a second major QTL on chromosome 6DL (QRlnt.lrc-6D.2) that explained 8.3-13.4% of the phenotypic variation for P. thornei resistance. An additional major QTL associated with P. neglectus resistance was detected on chromosome 4DS (QRlnn.lrc-4D.1) and explained a further 10.3-15.4% of the phenotypic variation. The identification and tagging of nematode resistance genes with molecular markers will allow appropriate allele combinations to be selected, which will aid the successful breeding of wheat with dual nematode resistance.Additional keywords: synthetic hexaploid wheat, doubled haploids, composite interval mapping. IntroductionTwo species of root-lesion nematode, Pratylenchus thornei Sher and Allen and P. neglectus (Rensch) Filipjev and Schuurmans Stekhoven, cause substantial yield losses in cereal crops throughout the world. High populations of P. thornei cause up to 85% yield loss in wheat (Triticum aestivum L.) varieties (Thompson et al. 1993). Similarly, P. neglectus causes up to 27% yield loss in wheat varieties (Taylor et al. 1999). In Australia, annual lost wheat production caused by P. thornei and P. neglectus is estimated at $36 million and $12 million, respectively (Brennan and Murray 1998).Glasshouse screening of wheat lines inoculated with pure cultures of nematodes has suggested that the genetic mechanisms that confer resistance to P. thornei do not confer resistance to P. neglectus (Farsi et al. 1995;Taylor et al.

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Section: Discussionmentioning

confidence: 84%

Section: Bsa and Framework Mapmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of quantitative trait loci for resistance to two species of root-lesion nematode (Pratylenchus thornei and P. neglectus) in wheat

Zwart

Thompson²,

Godwin

2005

Aust. J. Agric. Res.

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“…In Australia, many wheat accessions and varieties were identified to be resistant to P. thornei [32][33][34]. However, only one P. neglectus-resistant gene (Rlnn1) , which was located in chromosome 7AL and originated from Excalibur wheat, has been identified and validated so far [35]. Studies of descents of W-7984 (resistant) × Opata85 (susceptible), CPI133872 (resistant) × Janz (susceptible), AUS13124 (resistant) × Janz (susceptible), AUS4926 (resistant) × Janz showed many quantitative traits loci (QTL) with resistance to P. neglectus and/or P. thornei.…”

Section: Resistance Genesmentioning

confidence: 99%

A Review of Root Lesion Nematode: Identification and Plant Resistance

Yu¹,

Liu²,

Zhu³

et al. 2012

AiM

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Root lesion nematode, one of the three most devastated plant parasitic nematodes, is widespread in the world. They can invade various food and cash crops, and cause great agriculture loss. Recognition and identification of root lesion nematode are important joint of nematode management, as well as the mechanism research of plant anti-nematode, which is of great benefit to breed resistance varieties. In this review, the recent advances in nematode identification and research of plant anti-nematode are presented, and the importance of non-inaccuracy identification of nematode was emphasized. The mechanism of resistance to root lesion nematode was also discussed.

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