Abstract:Root lesion nematode, one of the three most devastated plant parasitic nematodes, is widespread in the world. They can invade various food and cash crops, and cause great agriculture loss. Recognition and identification of root lesion nematode are important joint of nematode management, as well as the mechanism research of plant anti-nematode, which is of great benefit to breed resistance varieties. In this review, the recent advances in nematode identification and research of plant anti-nematode are presented… Show more
“…However, intraspecific variability in Pratylenchus spp. has been pointed out as one of the main difficulties in the taxonomy of this group since most of the characters used for identifying the species are very variable (YU et al, 2012;MOKRINI et al, 2013;NORONHA et al, 2017).…”
The root-lesion nematodes Pratylenchus spp. have a wide geographic distribution and a large number of host plants. These migratory endoparasites are found associated with sugarcane around the world causing diseases that induce permanent losses in productivity. Although this genus has ordinarily been reported in low-producing sugarcane areas in northeastern Brazil, studies of the species occurrence are few and restricted to a very low number of sites and samples. This study aimed to morphologically and morphometrically characterize Pratylenchus species from different sugarcane regions of Pernambuco state, where composite samples of soil and roots were collected on 30 farms from three sugarcane mills. The external morphological characters of the nematode body from different populations had a diagnostic value compatible with those obtained in the literature, showing the dominance of P. zeae (87%) in the region. Atypical populations of the genus Pratylenchus were also found.
“…However, intraspecific variability in Pratylenchus spp. has been pointed out as one of the main difficulties in the taxonomy of this group since most of the characters used for identifying the species are very variable (YU et al, 2012;MOKRINI et al, 2013;NORONHA et al, 2017).…”
The root-lesion nematodes Pratylenchus spp. have a wide geographic distribution and a large number of host plants. These migratory endoparasites are found associated with sugarcane around the world causing diseases that induce permanent losses in productivity. Although this genus has ordinarily been reported in low-producing sugarcane areas in northeastern Brazil, studies of the species occurrence are few and restricted to a very low number of sites and samples. This study aimed to morphologically and morphometrically characterize Pratylenchus species from different sugarcane regions of Pernambuco state, where composite samples of soil and roots were collected on 30 farms from three sugarcane mills. The external morphological characters of the nematode body from different populations had a diagnostic value compatible with those obtained in the literature, showing the dominance of P. zeae (87%) in the region. Atypical populations of the genus Pratylenchus were also found.
“…identification is needed. DNA-based techniques have been used previously to discriminate species of plant-parasitic nematodes (Oliveira et al 2011;Yu et al 2012). Pinochet et al (1994) reported the use of random amplified polymorphic DNA polymerase chain reaction (PCR) analysis to differentiate isolates of P. vulnus.…”
Pratylenchus scribneri is a plant-parasitic root-lesion nematode causing economic damage to various crops worldwide. Identifying root-lesion nematodes to species using traditional morphological methods is an arduous task requiring extensive training on nematode taxonomy and years of experience. Thus, molecular methods for P. scribneri detection and identification were developed. Conventional and real-time polymerase chain reaction (PCR) assays with new species-specific primers were used in this study, which exclusively amplified DNA of P. scribneri but not DNA from other Pratylenchus spp. or non-Pratylenchus spp. tested. Compared with conventional PCR that was able to detect an equivalent to 1/4 of the DNA of a single nematode, real-time PCR was more sensitive and could amplify an equivalent to 1/128 of the DNA of one nematode. Both conventional and real-time PCR assays successfully identified P. scribneri and distinguished it from P. penetrans and P. neglectus isolated from field samples collected from various locations in North Dakota and Minnesota. The Blast-search based on the sequence information confirmed the reliability of the PCR assays for species identification. This is the first report of P. scribneri identification using a real-time PCR assay. The developed PCR assays are suitable for use in diagnostic laboratories and detection of field infestations with this nematode species.
“…Recently, ramie leaves and shoots have also been used as fodder for beef cattle and geese because of the plant’s high crude protein content [ 21 ]. Root lesion disease (RLD), a destructive root disease that is caused by the nematode Pratylenchus coffeae , severely impairs the growth and yield of ramie [ 22 ]. The lack of knowledge on ramie RLD resistance mechanisms or genes severely hinders efforts in effectively breeding and utilizing nematode-resistant ramie.…”
Phytocystatins play multiple roles in plant growth, development and resistance to pests and other environmental stresses. A ramie (Boehmeria nivea L.) phytocystatin gene, designated as BnCPI, was isolated from a ramie cDNA library and its full-length cDNA was obtained by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence (691 bp) consisted of a 303 bp open reading frame (ORF) encoding a protein of 100 amino acids with deduced molecular mass of 11.06 kDa and a theoretical isoelectric point (pI) of 6.0. The alignment of genome DNA (accession No. MF153097) and cDNA sequences of BnCPI showed that an intron (~104 bp) exists in the coding region. The BnCPI protein contains most of the highly conserved blocks including Gly5-Gly6 at the N-terminal, the reactive site motif QxVxG (Q49V50V51S52G53), the L79-W80 block and the [LVI]-[AGT]-[RKE]-[FY]-[AS]-[VI]-x-[EDQV]-[HYFQ]-N (L22G23R24 F25A26V27 D28D29H30 N31) block that is common among plant cystatins. BLAST analysis indicated that BnCPI is similar to cystatins from Glycine max (77%), Glycine soja (76%), Hevea brasiliensis (75%) and Ricinus communis (75%). The BnCPI was subcloned into expression vector pSmart-I and then overexpressed in Escherichia coli BL21 (DE3) as a His-tagged recombinant protein. The purified reBnCPI has a molecular mass of 11.4 kDa determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE). Purified reBnCPI can efficiently inhibit the protease activity of papain and ficin toward BANA (Nα-benzoyl-L-arginine-2-naphthyamide), as well as the mycelium growth of some important plant pathogenic fungi. The data further contribute to our understanding of the molecular functions of BnCPI.
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