Mapping of the metal intake in plants by large-field X-ray microradiography and preliminary feasibility studies in�microtomography

Kaiser, Jozef; Reale, L.; Ritucci, A.; Tomassetti, Giuseppe; Poma, Anna; Spanó, Laura; Tucci, Adele; Flora, F.; Lai, Antonia; Faenov, A. Ya.; Pikuz, T. A.; Mancini, Lucia; Tromba, Giuliana; Zanini, Franco

doi:10.1140/epjd/e2004-00183-2

Cited by 54 publications

(27 citation statements)

References 13 publications

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“…This process is very similar to that we could observe in the case of Cu accumulation in Dyplotaxis erucoides (Kaiser et al, 2005). Moreover, it seems that there is also a different intake process in the stem of Z. mays in comparison with the Helianthus annuus as it results from a dual-energy study previously conducted on this sunflower sample (Kaiser et al, 2007).…”

Section: Resultssupporting

confidence: 86%

“…We highlighted the intake in the epidermis of the stem of plants treated with three (Kaiser et al, 2005(Kaiser et al, , 2007, we found the same process of intake in the epidermis for the first plant and a different mechanism for the second.…”

Section: Discussionmentioning

confidence: 60%

“…A detailed account of the dual-energy algorithm and its implementation for elemental mapping were given in our earlier articles (Kaiser et al, 2005(Kaiser et al, , 2007Reale et al, 2008). In those experiments, dual-energy X-ray microradiography were tested on different plant samples grown in laboratory conditions with contaminants (e.g., Pb, Cd, Cu) added in controlled doses.…”

Section: The Dual-energy Algorithmmentioning

confidence: 99%

“…Moreover, synchrotron radiation can be tuned at the desired wavelength, and because of its high intensity, it is also able to detect very low amount of contaminants. Different techniques can be applied using X-rays from synchrotron sources: dual-energy (i.e., imaging of samples at X-ray energy above and below the edge of the heavy elements under study) radiography and tomography (Kaiser et al, 2005(Kaiser et al, , 2007Reale et al, 2008), projection microscopy with zone plate lenses (Yada 2009;Awaji et al, 2001), diffraction microscopy (Shapiro et al, 2005), X-ray scanning transmission microscopy (Hornberger et al, 2007), as well as X-ray microfluorescence.…”

Section: Introductionmentioning

confidence: 99%

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Detection of lead in Zea mays by dual‐energy X‐ray microtomography at the SYRMEP beamline of the ELETTRA synchrotron and by atomic absorption spectroscopy

Reale

Kaiser

Pace

et al. 2009

Microscopy Res & Technique

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This study is related to the application of the X-ray dual-energy microradiography technique together with the atomic absorption spectroscopy (AAS) for the detection of lead on Zea mays stem, ear, root, and leaf samples. To highlight the places with lead intake, the planar radiographs taken with monochromatic X-ray radiation in absorption regime with photon energy below and above the absorption edge of a given chemical element, respectively, are analyzed and processed. To recognize the biological structures involved in the intake, the dual-energy images with the lead signal have been compared with the optical images of the same Z. mays stem. The ear, stem, root, and leaf samples have also been analyzed with the AAS technique to measure the exact amount of the hyperaccumulated lead. The AAS measurement revealed that the highest intake occurred in the roots while the lowest in the maize ears and in the leaf. It seems there is a particular mechanism that protects the seeds and the leaves in the intake process.

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Section: Resultssupporting

confidence: 86%

Section: Discussionmentioning

confidence: 60%

Section: The Dual-energy Algorithmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of lead in Zea mays by dual‐energy X‐ray microtomography at the SYRMEP beamline of the ELETTRA synchrotron and by atomic absorption spectroscopy

Reale

Kaiser

Pace

et al. 2009

Microscopy Res & Technique

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“…The measurements presented in this work gave the map of the intensity (or area) of a spectral line, which may be proportional to the local concentration, but there was not attempt to calibration or a demonstration of linearity of response. In the ongoing work complimentary techniques, mainly X-ray microradiography and microtomography (Kaiser et al, 2005 will be applied for validation of LIBS and LA-ICP-MS outcomes. …”

Section: Resultsmentioning

confidence: 99%

Utilization of laser‐assisted analytical methods for monitoring of lead and nutrition elements distribution in fresh and dried Capsicum annuum l. leaves

Galiová

Kaiser

Novotný

et al. 2010

Microscopy Res & Technique

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Laser induced breakdown spectroscopy (LIBS) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) have been applied for high-resolution mapping of accumulation and distribution of heavy metal (lead) and nutrition elements (potassium, manganese) in leaves of Capsicum annuum L. samples. Lead was added in a form of Pb(NO₃)₂ at concentration up to 10 mmol L⁻¹ into the vessels that contained tap water and where the 2-months old Capsicum annuum L. plants were grown another seven days. Two dimensional maps of the elements are presented for both laser-assisted analytical methods. Elemental mapping performed on fresh (frozen) and dried Capsicum annuum L. leaves are compared.

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Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions

et al. 2010

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The main aim of this paper is to utilize high performance liquid chromatography with electrochemical detection for determination of thiols content in plants tissues of lettuce treated with lead(II) ions (0, 0.5 and 1 mM). We used two HPLC-ED instruments: HPLC coupled with one channel amperometric detector and HPLC coupled with twelve channel coulometric detector to detect simultaneously twelve thiols. The detection limits of thiols measured by CoulArray detector were about two magnitudes lower in comparison to those measured by Coulochem III detector and were from tens to hundreds pM. Under the optimal conditions, we utilized HPLC-CoulArray detector for analysis of tissues from lettuce plants. In addition, distribution and accumulation of lead ions with high spatial resolution was monitored using laser induced breakdown spectroscopy.

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Mapping of the metal intake in plants by large-field X-ray microradiography and preliminary feasibility studies in�microtomography

Cited by 54 publications

References 13 publications

Detection of lead in Zea mays by dual‐energy X‐ray microtomography at the SYRMEP beamline of the ELETTRA synchrotron and by atomic absorption spectroscopy

Detection of lead in Zea mays by dual‐energy X‐ray microtomography at the SYRMEP beamline of the ELETTRA synchrotron and by atomic absorption spectroscopy

Utilization of laser‐assisted analytical methods for monitoring of lead and nutrition elements distribution in fresh and dried Capsicum annuum l. leaves

Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions

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