Mapping and validation of quantitative trait loci associated with wheat yellow mosaic bymovirus resistance in bread wheat

Zhu, Xiaobiao; Wang, Haiyan; Guo, Jiao; Wu, Zhenzhen; Cao, Aizhong; Bie, Tongde; Nie, Mingjuan; You, Frank M.; Cheng, Zhiyuan; Xiao, Jin; Liu, Yangyang; Cheng, Shunhe; Chen, Peidu; Wang, Xiue

doi:10.1007/s00122-011-1696-3

Cited by 27 publications

(20 citation statements)

References 33 publications

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“…The best effective method to control the disease is to grow resistant wheat varieties, but there are few known resistance genes in current wheat cultivars. It has been reported that WYMV resistance in wheat is controlled by one to three genes (Qin et al, 1986) with one major gene being associated with homologous group 2 chromosome (Liu et al, 2005a;Nishio et al, 2010;Zhu et al, 2012). Furthermore, genetic analyses showed that the inheritance of this resistance is complex and influenced by many factors (Zhou et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Durable field resistance to wheat yellow mosaic virus in transgenic wheat containing the antisense virus polymerase gene

Chen¹,

Sun²,

HongYa³

et al. 2013

Plant Biotechnology Journal

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Summary Wheat yellow mosaic virus (WYMV) has spread rapidly and causes serious yield losses in the major wheat‐growing areas in China. Because it is vectored by the fungus‐like organism Polymyxa graminis that survives for long periods in soil, it is difficult to eliminate by conventional crop management or fungicides. There is also only limited resistance in commercial cultivars. In this research, fourteen independent transgenic events were obtained by co‐transformation with the antisense NIb8 gene (the NIb replicase of WYMV) and a selectable gene bar. Four original transgenic lines (N12, N13, N14 and N15) and an offspring line (N12‐1) showed high and durable resistance to WYMV in the field. Four resistant lines were shown to have segregated and only contain NIb8 (without bar) by PCR and herbicide resistance testing in the later generations. Line N12‐1 showed broad‐spectrum resistance to WYMV isolates from different sites in China. After growing in the infested soil, WYMV could not be detected by tissue printing and Western blot assays of transgenic wheat. The grain yield of transgenic wheat was about 10% greater than the wild‐type susceptible control. Northern blot and small RNA deep sequencing analyses showed that there was no accumulation of small interfering RNAs targeting the NIb8 gene in transgenic wheat plants, suggesting that transgene RNA silencing, a common mechanism of virus‐derived disease resistance, is not involved in the process of WYMV resistance. This durable and broad‐spectrum resistance to WYMV in transgenic wheat will be useful for alleviating the damage caused by WYMV.

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Section: Introductionmentioning

confidence: 99%

Durable field resistance to wheat yellow mosaic virus in transgenic wheat containing the antisense virus polymerase gene

Chen¹,

Sun²,

HongYa³

et al. 2013

Plant Biotechnology Journal

Self Cite

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“…In our study, we did not detect any QTL in other important groups such as the group 5 [Sbm1 for SBCMV resistance in bread wheat on 5DL (Bayles et al 2007)] or the group 3 [Qym2 for WYMV resistance in bread wheat (Zhu et al 2012)]. Therefore, multiple sources of resistance exist for these P. graminis transmitted viruses (Online Resource 11).…”

Section: Discussionmentioning

confidence: 58%

“…This region was projected between 0 and 27.6 cM on our high-resolution consensus genetic map, suggesting that Qssm - mtpsa - 7BS differs from Qsbm-ubo-7BS, but further investigations are needed to sort out this question. Another minor QTL concerning the WYMV resistance of bread wheat was described by Zhu et al (2012) on chromosome 7B, alongside marker Xgwm46 . This marker is located at 54.7 cM on our consensus genetic map, colocalizing near Qssm - mtpsa - 7BS .…”

Section: Discussionmentioning

confidence: 92%

Epistatic determinism of durum wheat resistance to the wheat spindle streak mosaic virus

et al. 2017

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Key message The resistance of durum wheat to the Wheat spindle streak mosaic virus (WSSMV) is controlled by two main QTLs on chromosomes 7A and 7B, with a huge epistatic effect. Abstract Wheat spindle streak mosaic virus (WSSMV) is a major disease of durum wheat in Europe and North America. Breeding WSSMV-resistant cultivars is currently the only way to control the virus since no treatment is available. This paper reports studies of the inheritance of WSSMV resistance using two related durum wheat populations obtained by crossing two elite cultivars with a WSSMV-resistant emmer cultivar. In 2012 and 2015, 354 recombinant inbred lines (RIL) were phenotyped using visual notations, ELISA and qPCR and genotyped using locus targeted capture and sequencing. This allowed us to build a consensus genetic map of 8568 markers and identify three chromosomal regions involved in WSSMV resistance. Two major regions (located on chromosomes 7A and 7B) jointly explain, on the basis of epistatic interactions, up to 43% of the phenotypic variation. Flanking sequences of our genetic markers are provided to facilitate future marker-assisted selection of WSSMV-resistant cultivars.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-017-2904-6) contains supplementary material, which is available to authorized users.

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“…A study by researchers in China also identified a major WYMV‐resistant QTL on chromosome 5A of the Japanese variety ‘Xifeng Wheat’ (‘Nishikaze Komugi’), but the WYMV pathotype was not specified (Zhu et al. ). However, no QTL was detected in this region in our material.…”

Section: Resultsmentioning

confidence: 99%

Identification and validation of a quantitative trait locus associated with wheat yellow mosaic virus pathotype I resistance in a Japanese wheat variety

et al. 2015

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Yellow mosaic disease, caused by wheat yellow mosaic virus (WYMV), is one of the most serious diseases of winter wheat (Triticum aestivum L.) in Japan. The three pathotypes of WYMV are distributed in different geographical areas: pathotype I is found mainly in western and central Japan (Kanto), pathotype II in northern Japan (Tohoku and Hokkaido) and pathotype III on the southern island of Japan (Kyushu). A total of 246 doubledhaploid (DH) lines, derived from a cross between 'Yumechikara' (resistant) and 'Kitahonami' (susceptible), were evaluated for 2 years for their resistance to WYMV pathotype I. A single major quantitative trait locus, Q.Ymym, mapping to chromosome 2D was associated with resistance to pathotype I in 'Yumechikara'. This is the first time a QTL responsible for pathotype I resistance has been identified. Fine mapping of Q.Ymym indicated that it was on a tight linkage block originating from 'Yumechikara', and the markers associated with this block will accelerate the development of varieties resistant to WYMV pathotype I.

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Mapping and validation of quantitative trait loci associated with wheat yellow mosaic bymovirus resistance in bread wheat

Cited by 27 publications

References 33 publications

Durable field resistance to wheat yellow mosaic virus in transgenic wheat containing the antisense virus polymerase gene

Durable field resistance to wheat yellow mosaic virus in transgenic wheat containing the antisense virus polymerase gene

Epistatic determinism of durum wheat resistance to the wheat spindle streak mosaic virus

Identification and validation of a quantitative trait locus associated with wheat yellow mosaic virus pathotype I resistance in a Japanese wheat variety

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