Manufacture of bioactive peptide-rich concentrates from Whey: Characterization of pilot process

Identification of angiotensin converting enzyme inhibitory and antioxidant peptides in a whey protein concentrate hydrolysate produced at semi‐pilot scale

“…The active peptides therein were RYLGY and AYFYPEL. Peptides produced during the Cynara cardunculus proteinase hydrolysis of whey protein at pilot scale had potent ACE inhibitory activity (Tavares et al, 2012). A UF fraction of a whey protein isolate hydrolysate produced at pilot scale (400 L) had ACE inhibitory activity and the ability to chelate iron in solution (O'Loughlin et al, 2014).…”

“…In order for food protein hydrolysis to be adopted as a viable approach in the production of bioactive peptides, it is essential that this process is transferable to large scale. However, a limited number of studies appear in the literature demonstrating the enzymatic production and characteristics of bioactive peptides generated at semi‐pilot scale (Contreras et al ., ; Tavares et al ., ; O'Loughlin et al ., ). Therefore, the objectives of this study were (i) to transfer a previously described laboratory‐scale process (O'Keeffe & FitzGerald, ) to semi‐pilot scale for the Corolase ® PP hydrolysis and ultrafiltration of whey protein concentrate, (ii) to further fractionate (using laboratory‐scale ultrafiltration and SP RP‐HPLC) and analyse the bioactivities (ACE inhibitory and antioxidant activities) within the semi‐pilot scale 5 kDa permeate and (iii) to employ UPLC‐MS/MS to identify the peptides which may be responsible for the observed bioactivities.…”

Evaluation of Antioxidant, Antiglycant and ACE-Inhibitory Activity in Enzymatic Hydrolysates of <i>α</i>-Lactalbumin

“…Food industry has invested in the revalorization of its effluents to create added-value products by investigating their biologically beneficial properties [4]. α-lactalbumin is the second major protein of whey proteins which has a globular structure with 123 residues and two subdomains (α with 4-helices and β with β-sheets and loop regions) [5]; [6], a molecular weight of 14 -17 kDa and an isoelectric point of 4.2 [6], four disulfide bonds and a calcium ion [7].…”

Dual function peptides from pepsin hydrolysates of whey protein isolate

“…Whey proteins are being extensively studied for the last decades, especially after the consolidation of technologies for processing whey into concentrated or isolated protein-enriched ingredients, giving rise to valorized products for food, nutraceutical and pharmaceutical industries (Tavares et al, 2012;Tavares & Malcata, 2013).…”