Dual function peptides from pepsin hydrolysates of whey protein isolate

Digestive fate of dietary carrageenan: Evidence of interference with digestive proteolysis and disruption of gut epithelial function

“…Moreover, the formation of low MW bands (marked in red boxes) varied between the various CGN‐WPI mixtures, and was different from that obtained for pure WPI. In line with previous reports , low enzymatic degradation of β‐lactoglobulin and high susceptibility of α‐lactalbumin were observed. However, this pattern was altered following the addition of the different types of CGN.…”

Application of preliminary high-pressure processing for improving bioactive characteristics and reducing antigenicity of whey protein hydrolysates

“…Peptides released during enzymatic hydrolysis of the WPC have been consistently described as efficient vasodilatory agents. They may be helpful to reinforce the antihypertensive effects of classical pharmacological agents, inducing vascular relaxation throughout different mechanisms, such as inhibition of the angiotensin-converting enzyme (ACE), stimulation and increased bioavailability of nitric oxide (NO), and modulation of reactive oxygen species, among others (Chakrabarti and Wu, 2016; Mellinger-Silva et al., 2015; Ozorio et al., 2019).…”

“…All procedures were previously approved by the local Animal Ethics Committee (5371190815). The animals were euthanized by anesthetic overdose (ketamine/xylazine; over 140/20 mg/kg, intraperitoneally), and the thoracic aortas were collected and prepared for functional experiments in organ baths containing physiological saline solution (composition in mmol/L: NaCl 131.3, KCl 4.7, KH 2 PO 4 1.18, MgSO 4 7H 2 O 1.17, NaHCO 3 14.9, D-glucose 5.5, CaCl 2 1.6, EDTA 0.08; pH 7.4) at 37 °C and continuously aerated with 5% CO 2 /95%O 2 , as previously detailed (Mellinger-Silva et al., 2015). Briefly, the preparations were subjected to a basal tone of 1 g and allowed to stabilize into the organ baths for 1 h without addition of any drug.…”

Gastrointestinal digestion enhances the endothelium-dependent vasodilation of a whey hydrolysate in rat aortic rings

“…In this sense, the present study aimed to investigate the hypothesis that biochemical transformations occurring in whey protein hydrolysate during gastrointestinal digestion may result in different profiles of vascular effect. To explore this hypothesis, the whey protein hydrolysate was subjected to a simulated gastrointestinal digestion, and the vascular activity of the resulting products was evaluated in rat aortic rings maintained in organ baths (Mellinger-Silva et al, 2015). Our results disclosed a new piece of evidence regarding the vasorelaxant potential of whey peptides.…”

“…Whey peptides may lower blood pressure (BP) through different mechanisms, such as inhibition of angiotensin-I converting enzyme (ACE), renin and pro-renin, blockage of calcium channels and AT1 receptors, and stimulation of nitric oxide production (Udenigwe & Mohan, 2014). In this sense, our research group started to investigate the relationship between different technological conditions, including scaling up the process and the comparison of drying methods with the preservation of the vasorelaxant activity of whey peptides (Mellinger-Silva et al, 2015;Ozorio, Pereira, et al, 2019;Ozorio, Silva, et al, 2019).…”