Manometric assay and properties of pancreatic cholesterol esterase

Korzenovsky, Mitchell; Diller, E. R.; Marshall, Anne; Auda, Barbara M.

doi:10.1042/bj0760238

Cited by 26 publications

(1 citation statement)

References 14 publications

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“…Although this enzyme was the object of re search by several groups [5-8], our knowledge is still rather poor, obviously caused by the dif ficulties of its measurement. The examiners cited above used physical [6], chemical [5,8], or radiochemical [1][2][3][4][5]7] methods, which are known to be inconvenient as well as rather insensitive. Also titration of the fatty acids released by CEH [pH-stat method, 9] is not suitable for studies concerning this enzyme.…”

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confidence: 99%

Lipolytic Enzymes of the Human Pancreas

Meyer-Bertenrath¹

1982

Enzyme

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Cholesterol erster hydrolase (cholesterol esterase, EC 3.1.1.13) does not only act on cholesterol esters, but also on esters of fluorescein. Diesters as well as monoesters of this dye show neither color nor fluorescence, so that these compounds may be used as chromogenic or fluorogenic substrates. Splitting off the ester bonds immediately causes the return of the original properties of the fluorescein. Its dilaurate was found to be a specific substrate of cholesterol esterase, allowing the measurement of activities of this enzyme down to ranges of 0.01 U/l by a fluorometric assay.

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confidence: 99%

Lipolytic Enzymes of the Human Pancreas

Meyer-Bertenrath¹

1982

Enzyme

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Enzymatic Synthesis and Hydrolysis of Cholesterol Esters

Vahouny

Treadwell

1968

Methods of Biochemical Analysis

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A simplified biological synthesis of labeled cholesteryl esters

Kothari

Carlton

Kritchevsky

1973

J Label Compd Radiopharm

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A SIMPLIFIED BIOLOGICAL SYNTHESIS OF LABaED CHOLESTERYL ESTERS, Hirnanshu V. K o t h a r i , J a n e t S. C a r l t o n a n d D a v i d K r i t c h e v s k y . H a r r i s o n Dept. o f S u r g i c a l R e s e a r c h , U n i v e r s i t y o f P e n n s y l v a n i a , S c h o o l of M e d i c i n e a n d t h e W i s t a r I n s t i t u t e o f A n a t o m y and B i o l o g y , P h i l a d e l p h i a , P A 19104. R e c e i v e d o n J a n u a r y 2 2 , 1973. S UMMA R Y A simplified procedure i s described for the synthesis and p u r i f i c a t i o n of labelled cholesteryl e s t e r s . The e s t e r s are synthesized enzymatically from (4-CI cholesterol and f r e e f a t t y acids. 1 4 The separation of the l i p i d s from aqueous medium i s simplif i e d by lyophilizing the reaction mixture a t equilibrium. The l i p i d components of the freeze-dried powder are separated by s i l i c i c acid chromatography. The radiochemical purity of t h e product was shown t o be greater than 98X.

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Manometric assay and properties of pancreatic cholesterol esterase

Cited by 26 publications

References 14 publications

Lipolytic Enzymes of the Human Pancreas

Lipolytic Enzymes of the Human Pancreas

Enzymatic Synthesis and Hydrolysis of Cholesterol Esters

A simplified biological synthesis of labeled cholesteryl esters

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