Enzymatic Synthesis and Hydrolysis of Cholesterol Esters

Vahouny, George V.; Treadwell, C. R.

doi:10.1002/9780470110348.ch4

Cited by 30 publications

(1 citation statement)

References 114 publications

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“…Cholesterol oxidase belongs to flavin-specific oxidoreductases family. Its prosthetic group FAD is covalently attached to the core protein in Streptomyces and is non-covalently bound to the core enzyme in Brevibacterium [18]. Cholesterol esterase catalyses the hydrolysis of sterol esters into sterols and fatty acids.…”

Section: Enzymes Involved In Fabrication Of Cholesterol Biosensormentioning

confidence: 99%

Recent approaches to ameliorate selectivity and sensitivity of enzyme based cholesterol biosensors: a review

Gahlaut

Hooda

Dhull

2017

Artificial Cells, Nanomedicine, and Biotechnology

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The healthcare area is often reluctant to execute new technology unless they are proven to be safe, constructive and secure. Eventually, an aspiration stands for providing point-of-care testing service to allow a better estimation of the biochemical levels of a patient that entails an insistent remedial action. With increasing mortality rate due to cardiovascular diseases (CVDs) in present scenario, it has become the need of hour to develop more advance methods for their diagnosis, so that it can be determined at sensitive levels and can be prevented from being fatal. Elevated level of cholesterol in blood stream is one of the utmost risk factors which lead to CVDs. Discernible from the vast research in this field, worth of cholesterol biosensors is already recognized and flourished in the clinical analysis of brain and cardiac vascular diseases. It necessitates unremitting progress in the development of biosensing technology towards fabrication, miniaturization and multiplexing ability of cholesterol quantification devices so that they can endow with lab-on-chip-analysis systems to the medical field. Different strategies have been meticulously explored for the engineering of cholesterol biosensors utilizing nanocomposites, conducting polymers, nanotubes and nanoparticles. Foremost, this article reviews the contemporary evolution in cholesterol biosensors, which encompass various strategies for immobilization of enzymes and roles of various matrices and artificial mediators used for the biosensor fabrication. Still there remains an enormous challenge to congregate the demands of performance and yield in a cost effective manner for its application in successful treatments of CVDs.

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Section: Enzymes Involved In Fabrication Of Cholesterol Biosensormentioning

confidence: 99%