A simplified biological synthesis of labeled cholesteryl esters

Abstract

A SIMPLIFIED BIOLOGICAL SYNTHESIS OF LABaED CHOLESTERYL ESTERS, Hirnanshu V. K o t h a r i , J a n e t S. C a r l t o n a n d D a v i d K r i t c h e v s k y . H a r r i s o n Dept. o f S u r g i c a l R e s e a r c h , U n i v e r s i t y o f P e n n s y l v a n i a , S c h o o l of M e d i c i n e a n d t h e W i s t a r I n s t i t u t e o f A n a t o m y and B i o l o g y , P h i l a d e l p h i a , P A 19104. R e c e i v e d o n J a n u a r y 2 2 , 1973. S UMMA R Y A simplified procedure i s described for the synthesis and p u r i f i c a t i o n of labelled cholesteryl e s t e r s . The e s t e r s are synthesized enzymatically from (4-CI cholesterol and f r e e f a t t y acids. 1 4 The separation of the l i p i d s from aqueous medium i s simplif i e d by lyophilizing the reaction mixture a t equilibrium. The l i p i d components of the freeze-dried powder are separated by s i l i c i c acid chromatography. The radiochemical purity of t h e product was shown t o be greater than 98X.