Mannose-specific legume lectin from the seeds of Dolichos lablab (FRIL) stimulates inflammatory and hypernociceptive processes in mice

Teixeira, Claudener S.; Assreuy, Ana Maria Sampaio; Osterne, Vinicius Jose Da Silva; Amorim, Renata Morais Ferreira; Brizeno, Luiz André Cavalcante; Debray, Henri; Nagano, Celso Shiniti; Delatorre, P.; Sampaio, Alexandre Holanda; Rocha, B.A.M.; Cavada, Benildo S.

doi:10.1016/j.procbio.2013.12.020

Cited by 19 publications

(6 citation statements)

References 31 publications

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“…The histopathological analysis of animal paws showed a characteristically acute inflammatory process that included severe infiltration of mixed leukocytes, changes in cytoarchitecture, edema and focal areas of hemorrhage. In addition, in silico assays confirmed that FRIL preferentially interacted with trimannoside that makes up the core N-glycans cell [87]. The antinociceptive properties of methanol extract of beans (fruits containing seeds) of …”

Section: Antiinflammatory and Analgesic Effectsmentioning

confidence: 87%

The pharmacology and medical importance of Dolichos lablab (Lablab purpureus)- A review

Al-Snafi¹

2017

IOSRPHR

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Abstract:-The phytochemical analysis of Dolichos lablab showed that it contained sugar, alcohols, phenols, steroids, essential oils, alkaloids, tannins, flavonoids, saponins, coumarins, terpenoids, pigments, glycosides, anthnanoids, wide range of minerals and many other metabolites. The preliminary pharmacological studies revealed that Dolichos lablab possessed antidiabetic, antiinflammatory, analgesic, antioxidant, cytotoxic, hypolipidemic, antimicrobial, insecticidal, hepatoprotective, antilithiatic, antispasmodic effects and also used for the treatment of iron deficiency anemia. The current review discussed the chemical constituents and pharmacological activities of Dolichos lablab.

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Section: Antiinflammatory and Analgesic Effectsmentioning

confidence: 87%

The pharmacology and medical importance of Dolichos lablab (Lablab purpureus)- A review

Al-Snafi¹

2017

IOSRPHR

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“…Biological activities elicited by lectins occur mainly by interaction of the lectin with the glycosylated region of their targets. 47,48 Accordingly, our previous study with VGL demonstrated the inhibition of the lectin's edematogenic activity by the association with its binding sugar. 19 Glycosylated regions in glycoproteins normally share the common core Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ1-Asn-X-Ser/Thr for N-glycans.…”

Section: Discussionmentioning

confidence: 97%

Vatairea guianensis lectin stimulates changes in gene expression and release of TNF‐α from rat peritoneal macrophages via glycoconjugate binding

Marques

Pires

Osterne

et al. 2021

J of Molecular Recognition

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Using a rat model of peritonitis, we herein report the inflammatory effect induced by the lectin isolated from Vatairea guianensis (VGL) seeds in the context of interactions between VGL and both toll-like receptor 4 (TLR4) and tumor necrosis factor receptor 1 (TNFR1). Peritoneal macrophages were stimulated with VGL for dose-dependent gene expression and release of TNF-α. In vivo results showed that VGL (1 mg/kg; intraperitoneal) induced peritonitis in female Wistar rats. Leukocyte migration, macrophage activation, and protein leakage were measured 3 and 6 hours after induction.In vitro, peritoneal macrophages were stimulated with VGL for gene expression and TNF-α dosage (mean ± SEM (n = 6), analysis of variance, and Bonferroni's test (P < .05)). In silico, VGL structure was applied in molecular docking with representative glycans. It was found that (a) VGL increases vascular permeability and stimulates leukocyte migration, both rolling and adhesion; (b) lectin-induced neutrophil migration occurs via macrophage stimulation, both in vitro and in vivo; (c) lectin interacts with TLR4 and TNFR1; and (d) stimulates TNF-α gene expression (RT-PCR) and release from peritoneal macrophages. Thus, upon lectin-glycan binding on the cell surface, our results suggest that VGL induces an acute inflammatory response, in turn activating the release of peritoneal macrophages via TNF-α and TLR and/or TNFR receptor pathways.

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“…Slices were observed by light microscopy coupled to image acquisition systems. The intensity of tissue in ammatory alterations was evaluated according to the following scores: (0) normal tissue -absence of edema and in ammatory cell in ltrate; (1) discrete tissue changes -mild edema and cellular in ltration; (2) moderate tissue alteration -signi cant edema and cellular in ltration; (3) intense tissue alteration -wide area of edema and cellular in ltration [13].…”

Section: Paw Edema Modelmentioning

confidence: 99%

The anti-inflamatory effect of Andira anthelmia lectin in rats involves inhibition of the prostanoid pathway, TNF-α and lectin domain

et al. 2022

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Objective: To investigate the effect and mechanisms of Andira anthelmia lectin in rat models of acute in ammation.Material: AAL anti-in ammatory activity was evaluated in Wistar rat models of paw edema, peritonitis and hyperalgesia.Methods: AAL (0.01-1 mg/kg i.v.) was injected 30 min before stimulation with carrageenan and with initial and late phase in ammatory mediators into the animals paw and peritoneum for evaluation of: cell migration (optical and intravital microscopy), paw edema (plethysmometry and histopathology); hyperalgesia (analgesimetry).Results: AAL inhibited leukocyte migration induced by carrageenan, mainly neutrophils to the peritoneal uid, decreasing leukocyte adhesion. In the peritoneal uid, AAL reduced the gene expression of TNF-α and cyclooxygenase, as well the levels of PGE 2 . ALL also inhibited the paw edema induced by carrageenan, mainly the late phase, but also the edema induced by serotonin, histamine, TNF-α, PLA 2 and PGE 2 , but not by L-arginine and bradykinin. In this model, AAL inhibited mechanical hypernociception induced by TNF-α, PGE 2 , db-cAMP and capsaicin, and the activity of myeloperoxidase in the paw tissues.Conclusion: AAL presents anti-in ammatory effect in acute models of rat in ammation involving the participation of prostaglandins, TNF-α and lectin domain.

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Mannose-specific legume lectin from the seeds of Dolichos lablab (FRIL) stimulates inflammatory and hypernociceptive processes in mice

Cited by 19 publications

References 31 publications

The pharmacology and medical importance of Dolichos lablab (Lablab purpureus)- A review

The pharmacology and medical importance of Dolichos lablab (Lablab purpureus)- A review

Vatairea guianensis lectin stimulates changes in gene expression and release of TNF‐α from rat peritoneal macrophages via glycoconjugate binding

The anti-inflamatory effect of Andira anthelmia lectin in rats involves inhibition of the prostanoid pathway, TNF-α and lectin domain

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