Vatairea guianensis lectin stimulates changes in gene expression and release of TNF‐α from rat peritoneal macrophages via glycoconjugate binding

Abstract

Using a rat model of peritonitis, we herein report the inflammatory effect induced by the lectin isolated from Vatairea guianensis (VGL) seeds in the context of interactions between VGL and both toll-like receptor 4 (TLR4) and tumor necrosis factor receptor 1 (TNFR1). Peritoneal macrophages were stimulated with VGL for dose-dependent gene expression and release of TNF-α. In vivo results showed that VGL (1 mg/kg; intraperitoneal) induced peritonitis in female Wistar rats. Leukocyte migration, macrophage activation, and protein leakage were measured 3 and 6 hours after induction.In vitro, peritoneal macrophages were stimulated with VGL for gene expression and TNF-α dosage (mean ± SEM (n = 6), analysis of variance, and Bonferroni's test (P < .05)). In silico, VGL structure was applied in molecular docking with representative glycans. It was found that (a) VGL increases vascular permeability and stimulates leukocyte migration, both rolling and adhesion; (b) lectin-induced neutrophil migration occurs via macrophage stimulation, both in vitro and in vivo; (c) lectin interacts with TLR4 and TNFR1; and (d) stimulates TNF-α gene expression (RT-PCR) and release from peritoneal macrophages. Thus, upon lectin-glycan binding on the cell surface, our results suggest that VGL induces an acute inflammatory response, in turn activating the release of peritoneal macrophages via TNF-α and TLR and/or TNFR receptor pathways.

The Antinociceptive Effect of Lonchocarpus Araripensis Lectin is Mediated by Endocannabinoid Receptors

“…It is well demonstrated that lectins have been used as tools in the study of inflammatory processes as they have the capacity of reversible and specific binding to glycan structures present in the membranes of inflammatory cells [24,25,26]. In the other study, LAL presented antiinflammatory activity via lectin domain, inhibiting neutrophil migration to rat peritoneal cavity and modulating inflammatory mediators [2].…”

A galactoside-specific Dalbergieae legume lectin from seeds of Vataireopsis araroba (Aguiar) Ducke

“…The characterization of these lectins allowed the division into three groups based on specificity, in particular the mannose/glucosespecific, the N-acetylgalactosamine/galactose-specific, and the N-acetylglucosamine-specific Dalbergieae lectins. Some of these proteins elicit interesting biological effects such as pro-and anti-inflammatory [6][7][8],…”

Antimicrobial Prenylated Isoflavones from the Leaves of the Amazonian Medicinal Plant Vatairea guianensis Aubl.

“…(Fabaceae, Papilionoideae) is a common tree in the Brazilian Amazon region, and its leaves and fruits are traditionally used for the treatment of dermatological mycosis. − The plant is commonly known as “fava de impingem”, “faveira amarela”, or “faveira grande do igapó” in Brazil . Because of its floral morphology, the genus Vatairea was previously assigned to the Dalbergieae tribe, but was recently reassigned to the unranked Vataireoid clade after DNA sequence based phylogenetic analyses. , Extensive pharmacological studies were performed on Vatairea guianensis lectin (VGL), which was obtained from its seeds and exhibited vasorelaxant and anti-inflammatory effects. ,, …”