Mannose-decorated hybrid nanoparticles for enhanced macrophage targeting

Abstract

Our goal was to design nanocarriers that specifically target and deliver therapeutics to polarized macrophages. Mannose receptors are highly overexpressed on polarized macrophages. In this study, we constructed Pluronic® -F127 polymer and tannic acid (TA) based nanoparticles (F127-TA core nanoparticles) with varying mannose densities. The particle size of the optimized mannose-decorated F127-TA hybrid nanoparticles (MDNPs) was found to be ~ 265 nm with a negative zeta potential of ~ − 4.5 mV. No significant changes in the size and zeta potentials of nanoparticles were observed, which demonstrated structural integrity and stability of the nanoformulation. Physicochemical characteristics of MDNPs were evaluated by FTIR and TGA and demonstrated the presence of mannose units on surface nanoparticles. A mannose-dependent cellular targeting and uptake of MDNPs was found in U937 macrophages. The uptake process was found to vary directly with time and volume of MDNPs nanoparticles. The uptake pattern is higher in M2 than M1. This behavior was also evident from the instantaneous and superior binding profile of M2 macrophage lysate protein with MDNPs over that of M1 macrophage lysate protein. These results demonstrated that an appropriate mannose ligand density was confirmed, suggesting efficient targeting of M2. Altogether, these data support that the MDNPs formulation could serve as a targeted therapeutic guide in the generation of nanomedicine to treat various conditions as an anti-inflammation therapy.

Targeting of Hepatic Macrophages by Therapeutic Nanoparticles

“…However, M2 macrophages show increased expression of mannose and galactose receptors (93). This makes it possible to target specifically anti-inflammatory phenotypes with mannosedecorated nanoparticles (94). Pluronic and chitosan-based NPs of 150 to 265 nm, decorated with mannose, with positive and negative zeta potentials, respectively, can selectively target M2 macrophages to treat inflammatory diseases and HIV infections.…”

“…Pluronic and chitosan-based NPs of 150 to 265 nm, decorated with mannose, with positive and negative zeta potentials, respectively, can selectively target M2 macrophages to treat inflammatory diseases and HIV infections. The charge of the NPs and their degree of internalization are dependent on mannose density (84,94).…”

Pectin-Tannic Acid Nano-Complexes Promote the Delivery and Bioactivity of Drugs in Pancreatic Cancer Cells

“…To prepare dye or drug loaded MPT-NCs, a simple solvent evaporation method was employed [36]. A detailed protocol was presented in our earlier publications [35,37,38]. In this method, the solutions of 150 µg 6-coumarin (in 150 µL acetone), or 150 µg drug (gemcitabine, 5-fluorouracil or irinotecan, in 150 µL acetone), or introduced in 0.5 mg of modified pectin (500 µg/1 mL) were mixed with 0.5 mg tannic acid.…”

“…In order to minimize the leaching factor, we performed experiments only for early time points (less than 6 h). Additionally, leached out dye would be precipitated in cell culture medium, which could be removed via centrifugation [37].…”

Polymer Capsules with Hydrophobic Liquid Cores as Functional Nanocarriers

“…Nanoscale DDS, especially polysaccharide-based, may be decorated by various ligands. Among the most promising ligands that may be used for targeted therapy, biotin, folic acid, mannose, pullulan and antibodies can be distinguished [ 129 , 130 , 131 , 132 ]. Yang et al described a vitamin-E-based nanoemulsion with promising properties as the theranostic platform.…”