Mannose-Binding Lectin ( MBL ) gene polymorphisms in susceptibility to pulmonary tuberculosis among the Lur population of Lorestan Province of Iran

Abstract

ObjectiveTuberculosis (TB) is caused by infection of Mycobacterium tuberculosis. Host genetic variability is an important determinant of the risk of developing TB in humans. Although the association between MBL polymorphisms and TB has been studied in various populations, the results are controversial. The aim of this study was to investigate mannose-binding lectin (MBL) gene polymorphisms with susceptibility to pulmonary tuberculosis (PTB) in a Lur population of Iran.MethodsIn this case-control study, four functional MBL gene polymorphisms (HL, XY, PQ and AB) were genotyped by using PCR Single Strand Conformation Polymorphism (SSCP) technique in a Lur population living in Lorestan Province, consisting of 100 patients with pulmonary tuberculosis (PTB) age and sex matched 100 healthy controls (HCs). Association analyses were performed with the SPSS 21 statistical software.ResultsWe found that MBL (HH) genotype polymorphism significantly was associated with increased susceptibility to TB (35% in patients vs. 22% in controls, P = 0.0417, OR = 1.909, %95 CI = 1.020–3.573). Additionally, H allele showed a significant association with increased risk of TB (56.5% in patients vs. 46% in controls, P = 0.0357, OR = 1.525, %95 CI = 1.028–2.262). Also, the distribution of L allele in patients was significantly lower frequency in TB patients compared to controls (43.5% vs. 54%, P = 0.0357, OR = 0.656, %95 CI = 0.442–0.973). However, the allelic and genotypic frequencies of AB, XY and PQ polymorphisms were not significantly different between the patients and the controls. We couldn't detect any significant differences between haplotypes among TB patients and healthy controls.ConclusionsOur findings demonstrated that HH genotype and H allele may increase the susceptibility to pulmonary TB in the Lur population of Iran, although L allele may decrease the susceptibility to pulmonary TB in this population. We suggest that it is necessary to further more studies with larger sample size and other ethnic population.

Association Study of MBL2 Gene Polymorphisms and Risk of Tuberculosis in Southeast of Iran

“…Li et al (2018) in a study on Chinese Uygur population found that MBL2 rs7095891 polymorphism was associated with an increased risk of TB; however, they did not find any association between rs1800450 as well as rs7096206 polymorphism and tuberculosis. Meanwhile, many studies have not found any association between MBL2 rs1800450 polymorphism and susceptibility or protection to tuberculosis (Soborg et al, 2007;Araújo et al, 2013;da Cruz et al, 2013;Wu et al, 2015;Amiri et al, 2017). The effect of MBL level on TB susceptibility has been controversial.…”

“…These polymorphisms changed the structure of MBL proteins causing a functional deficiency and reduction of stability; as such they were more rapidly degraded and thus reduced the serum level of MBL (Heitzeneder et al, 2012;Mandal et al, 2019). Also, it seems that these MBL proteins have less binding capacity to mannose and cannot activate complement via the lectin pathway (Amiri et al, 2017). However, Thye et al (2011) showed higher levels of MBL can promote infection by increasing the uptake of intracellular pathogens by phagocytes.…”

“…The MBL2 gene is located on chromosome 10 (10q11.2-q21) and contains many polymorphisms in the promoter or structural region of the gene affecting the expression level of MBL2 at transcription level. Low serum level of MBL2 is associated with increased susceptibility to infectious disease such as Mtb (Amiri et al, 2017). Several studies have shown that MBL2 polymorphisms are related to tuberculosis susceptibility though the results were inconsistent (Cao et al, 2018;Mandal et al, 2019;Tong et al, 2019).…”

A SNaPshot Assay for Determination of the Mannose-Binding Lectin Gene Variants and an Algorithm for Calculation of Haplogenotype Combinations

“…Up to this day, a wide spectrum of other methods have been used for detection of MBL2 gene variants, including commercially available TaqMan assays [ 27 ], high-resolution melt analysis (HRMA) [ 28 ], reverse hybridization with membrane-immobilized sequence-specific oligonucleotide probes (reverse PCR-SSOP) [ 29 ], PCR with sequence-specific primers (SSP-PCR) [ 30 , 31 ], PCR and restriction-fragment length polymorphism (PCR-RFLP) analysis [ 32 ], an oligonucleotide ligation assay [ 33 , 34 ], a single-strand conformation polymorphism technique (PCR-SSCP) [ 35 ], the iPLEX assay on a MassArray system [ 36 ], Sanger sequencing [ 37 ], and pyrosequencing [ 38 ].…”

Influence of Genetic Polymorphism Towards Pulmonary Tuberculosis Susceptibility

“…Moreover, LYPB haplotype showed a significant association with increased risk of TB among Chinese population ( 225 ). Whereas, in Iranian Lur population MBL H allele and HH genotype significantly associated with increased susceptibility to TB ( 226 ).…”